Determination of the DNA methylation level in tumor cells by capillary electrophoresis and laser-induced fluorescence detection

Electrophoresis

Volumn 25, Issue 6, 2004, Pages 839-845

  Wirtz, Michaela ^a   Stach, Dirk ^b   Kliem, Hans Christian ^b   Wiessler, Manfred ^b   Schmitz, Oliver J ^a  

^a UNIVERSITY OF WUPPERTAL   (Germany)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

5 Methylcytosine; Cancer; Capillary electrophoresis laser induced fluorescence; DNA adducts; DNA methylation

Indexed keywords

ALKYLATION; AMINES; DNA; ETHYLENE; FLUORESCENCE; LASER OPTICS; LASER PRODUCED PLASMAS; METHYLATION;

5-METHYLCYTOSINE; CANCER; CAPILLARY ELECTROPHORESIS-LASER-INDUCED FLUORESCENCE; DERIVATIZATIONS; DNA ADDUCTS; DNA METHYLATION; LASER INDUCED FLUORESCENCE; METHYLCYTOSINE; MONOPHOSPHATES; TUMOUR CELLS;

CAPILLARY ELECTROPHORESIS;

CYTIDINE DERIVATIVE; FLUORESCENT DYE; PHOSPHATE;

ANALYTIC METHOD; ARGON LASER; ARTICLE; CALCULATION; CANCER CELL CULTURE; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; DERIVATIZATION; DNA ISOLATION; DNA METHYLATION; FLUORESCENCE ANALYSIS; HUMAN; HUMAN CELL; HYDROLYSIS; MEASUREMENT; MICELLAR ELECTROKINETIC CHROMATOGRAPHY; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; QUANTUM YIELD; REPRODUCIBILITY;

EID: 1642566835     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.200305761     Document Type: Article

References (31)

1. Adorjan, P., Distler, J., Lipscher, E., Model, F., Muller, J., Pelet, C., Braun, A., Florl, A. R., Gutig, D., Grabs, G., Howe, A., Kursar, M., Lesche, R., Leu, E., Lewin, A., Maier, S., Muller, V., Otto, T., Scholz, C., Schulz, W. A., Seifert, H. H., Schwope, I., Ziebarth, H., Berlin, K., Piepenbrock, C., Olek, A., Nucleic Acids Res. 2002, 30, e21.
2. Baylin, S. B., Herman, J. G., Trends Genet. 2000, 16, 168-174.
3. Gama-Sosa, M. A., Slagel, V. A., Trewyn, R. W., Oxenhandler, R., Kuo, K. C., Gehrke, C. W., Ehrlich, M., Nucleic Acids Res. 1983, 11, 6883-6894.
4. Feinberg, A. P., Gehrke, C. W., Kuo, K. C., Ehrlich, M., Cancer Res. 1988, 48, 1159-1161.
5. Chen, R. Z., Pettersson, U., Beard, C., Jackson-Grusby, L., Jaenisch, R., Nature 1998, 395, 89-93.
6. Eden, A., Gaudet, F., Waghmare, A., Jaenisch, R., Science 2003, 300, 455.
7. Gaudet, F., Hodgson, J. G., Eden, A., Jackson-Grusby, L., Dausman, J., Gray, J. W., Leonhardt, H., Jaenisch, R., Science 2003, 300, 489-492.
8. Cravo, M., Pinto, R., Fidalgo, P., Chanves, P., Gloria, L., Nobre-Leitao, C., Costa Mira, F., Gut 1996, 39, 434-438.
9. Bariol, C., Suter, C., Cheong, K., Ku, S.-L., Meagher, A., Hawkins, N., Ward, R., Am. J. Pathol. 2003, 162, 1361-1371.
10. Iacopetta, B., Am. J. Pathol. 2003, 162, 1043-1045.
11. Plass, C., Soloway, P. D., Eur. J. Hum. Genet. 2002, 10, 6-16.
12. Frommer, M., McDonald, L. E., Millar, D. S., Collins, C. M., Watt, F., Grigg, G. W., Molloy, P. L., Paul, C. L., Proc. Natl. Acad. Sci. USA 1992, 89, 1827-1831.
13. Herman, J. G., Graff, J. R., Myöhänen, S., Nelkin, B. D., Baylin, S. B., Proc. Natl. Acad. Sci. USA 1996, 93, 9821-9826.
14. Gonzalgo, M. L., Jones, P. A., Nucleic Acids Res. 1997, 25, 2529-2531.
15. Sadri, R., Hornsby, P. J., Nucleic Acids Res. 1996, 24, 5058-5059.
16. Xiong, Z., Laird, P. W., Nucleic Acids Res. 1997, 25, 2532-2534.
17. Burri, N., Chaubert, P., BioTechniques 1999, 26, 233-234.
18. Suzuki, H., Itoh, F., Toyota, M., Kikuchi, T., Kakiuchi, H., Hinoda, Y., Imai, K., Electrophoresis 2000, 21, 904-908.
19. Eick, D., Fritz, H.-J., Doerfler, W., Anal. Biochem. 1983, 135, 165-171.
20. Oakeley, E. J., Schmitt, F., Jost, J.-P., BioTechniques 1999, 27, 744-752.
21. Fraga, M. F., Rodriguez, R., Canal, M. J., Electrophoresis 2000, 21, 2990-2994.
22. Frisco, S., Choi, S.-W., Dolnikowski, G. G., Selhub, J., Anal. Chem. 2002, 74, 4526-4531.
23. Fraga, M. F., Esteller, M., BioTechniques 2002, 33, 632-649.
24. Schmitz, O. J., Wörth, C. C. T., Stach, D., Wiessler, M., Angrew. Chem. Int. Ed. 2002, 41, 445-448.
25. Stach, D., Schmitz, O. J., Stilgenbauer, S., Benner, A., Döhner, H., Wiessler, M., Lyko, F., Nucleic Acids Res. 2003, 31, 1-6.
26. Godschalk, R., Nair, J., Kliem, H.-C., Wiessler, M., Bouvier, G., Bartsch, H., Chem. Res. Toxicol. 2002, 15, 433-437.
27. Hoffman, J. L., Biochemistry 1986, 25, 4444-4449.
28. Haugland, R. P., in: Spence, M. T. Z. (Ed.), Handbook of Fluorescent Probes and Research Chemicals, Molecular Probes Inc., Eugene, OR, 1996.
29. Seidel, C. A. M., Schulz, A., Sauer, M. H. M., J. Phys. Chem. 1996, 100, 5541-5553.
30. Steenken, S., Jovanovic, S. V., J. Am. Chem. Soc. 1997, 119, 617-618.
31. Lewis, F. D., Wu, T., Zhang, Y., Letsinger, R. L., Greenfield, S. R., Wasielewski, M. R., Science 1997, 277, 673-676.