Long Endogenous dsRNAs Can Induce Complete Gene Silencing in Mammalian Cells and Primary Cultures

Oligonucleotides

Volumn 13, Issue 5, 2003, Pages 381-392

  Diallo, M ^a   Arenz, C ^a   Schmitz, K ^a   Sandhoff, K ^a   Schepers, Ute ^a  

^a UNIVERSITY OF BONN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CERAMIDE GLUCOSYLTRANSFERASE; DOUBLE STRANDED RNA; GLUCOSYLCERAMIDASE; GLYCOSPHINGOLIPID; SMALL INTERFERING RNA; SPHINGOLIPID ACTIVATOR PROTEIN;

ANIMAL CELL; ARTICLE; CAENORHABDITIS ELEGANS; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; FIBROBLAST; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; KNOCKOUT GENE; MELANOMA CELL; MOUSE; NONHUMAN; NORTHERN BLOTTING; PLANT; POSTTRANSCRIPTIONAL GENE SILENCING; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVERSED PHASE LIQUID CHROMATOGRAPHY; RNA INTERFERENCE; SOMATIC CELL; THIN LAYER CHROMATOGRAPHY; WESTERN BLOTTING;

ANIMALS; BASE SEQUENCE; CELL LINE, TUMOR; CELLS, CULTURED; CLONING, MOLECULAR; GENE SILENCING; HELA CELLS; HUMANS; MAMMALS; MELANOMA, EXPERIMENTAL; MICE; RESTRICTION MAPPING; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, DOUBLE-STRANDED; RNA, SMALL INTERFERING; TRANSFECTION;

ANIMALIA; CAENORHABDITIS; CAENORHABDITIS ELEGANS; DROSOPHILA MELANOGASTER; MAMMALIA; MELANOGASTER;

EID: 1642564295     PISSN: 15454576     EISSN: None     Source Type: Journal    
DOI: 10.1089/154545703322617069     Document Type: Article

References (44)

1. ARENZ, C., and SCHEPERS, U. (2003). RNA interference: An ancient mechanism or a state of the art therapeutic application. Naturwissenschaften 90, 345-359.
2. ASHRAFI, K., CHANG, F.Y., WATTS, J.L., FRASER, A.G., KAMATH, R.S., AHRINGER, J., and RUVKUN, G. (2003). Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes. Nature 421, 268-272.
3. BERNSTEIN, E., CAUDY, A.A., HAMMOND, S.M., and HANNON, G.J. (2001). Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409, 363-366.
4. BRIDGE, A.J., PEBERNARD, S., DUCRAUX, A., NICOULAZ, A.L., and IGGO, R. (2003). Induction of an interferon response by RNAi vectors in mammalian cells. Nat. Genet. 34, 263-264.
5. BROWN, D.A., and LONDON, E. (2000). Structure and function of sphingolipid- and cholesterol-rich membrane rafts. J. Biol. Chem. 275, 17221-17224.
6. BRUMMELKAMP, T.R., BERNARDS, R., and AGAMI, R. (2002). A system for stable expression of short interfering RNAs in mammalian cells. Science 296, 550-553.
7. DIALLO, M., ARENZ, C., SCHMITZ, K., SANDHOFF, K., and SCHEPERS, U. (2003). RNA interference: A new method to analyze the function of glycoproteins and glycosylating proteins in mammalian cells. Knockout experiments with UDP-glucose/ceramide-glucosyltransferase. Methods Enzymol. 363, 173-190.
8. DONZE, O., and PICARD, D. (2002). RNA interference in mammalian cells using siRNAs synthesized with T7 RNA polymerase. Nucleic Acids Res. 30, e46.
9. ELBASHIR, S.M., HARBORTH, J., LENDECKEL, W., YALCIN, A., WEBER, K., and TUSCHL, T. (2001). Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494-498.
10. FIRE, A., XU, S.Q., MONTGOMERY, M.K., KOSTAS, S.A., DRIVER, S.E., and MELLO, C.C. (1998). Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806-811.
11. GONCZY, P., ECHEVERRI, C., OEGEMA, K., COULSON, A., JONES, S.J., COPLEY, R.R., DUPERON, J., OEGEMA, J., BREHM, M., CASSIN, E., HANNAK, E., KIRKHAM, M., PICHLER, S., FLOHRS, K., GOESSEN, A., LEIDEL, S., ALLEAUME, A.M., MARTIN, C., OZLU, N., BORK, P., and HYMAN, A.A. (2000). Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III. Nature 408, 331-336.
12. HAKOMORI, S. (1998). Cancer-associated glycosphingolipid antigens: Their structure, organization, and function. Acta Anat. 161, 79-90.
13. KAMATH, R.S., FRASER, A.G., DONG, Y., POULIN, G., DURBIN, R., GOTTA, M., KANAPIN, A., LE BOT, N., MORENO, S., SOHRMANN, M., WELCHMAN, D.P., ZIPPERLEN, P., and AHRINGER, J. (2003). Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature 421, 231-237.
14. KLEIN, A., HENSELER, M., KLEIN, C., SUZUKI, K., HARZER, K., and SANDHOFF, K. (1994). Sphingolipid activator protein D (sap-D) stimulates the lysosomal degradation of ceramide in vivo. Biochem. Biophys. Res. Commun. 200, 1440-1448.
15. KOLTER, T. and SANDHOFF, K. (1999). Sphingolipids - Their metabolic pathways and the pathobiochemistry of neurodegenerative diseases. Angew. Chem. Int. Ed. 38, 1532-1568.
16. LEE, N.S., DOHJIMA, T., BAUER, G., LI, H., LI, M.J., EHSANI, A., SALVATERRA, P., and ROSSI, J. (2002). Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat. Biotechnol. 20, 500-505.
17. LEE, S.B., GREEN, S.R., MATHEWS, M.B., and ESTEBAN, M. (1994). Activation of the double-stranded RNA (dsRNA)-activated human protein kinase in vivo in the absence of its dsRNA binding domain. Proc. Natl. Acad. Sci. USA 91, 10551-10555.
18. LEE, S.B., RODRIGUEZ, D., RODRIGUEZ, J.R., and ESTEBAN, M. (1997). The apoptosis pathway triggered by the interferon-induced protein kinase PKR requires the third basic domain, initiates upstream of Bcl-2, and involves ice-like proteases. Virology 231, 81-88.
19. MISQUITTA, L., and PATERSON, B.M. (1999). Targeted disruption of gene function in Drosophila by RNA interference (RNAi): A role for nautilus in embryonic somatic muscle formation. Proc. Natl. Acad. Sci. USA 96, 1451-1456.
20. MIYAGISHI, M., and TAIRA, K. (2002). U6 promoter driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nat. Biotechnol. 20, 497-500.
21. MOTLEY, A., BRIGHT, N., SEAMAN, M., and ROBINSON, M. (2003). Clathrin-mediated endocytosis in AP-2-depleted cells. J. Cell Biol. 162, 909-918.
22. NYKANEN, A., HALEY, B., and ZAMORE, P.D. (2001). ATP requirements and small interfering RNA structure in the RNA interference pathway. Cell 107, 309-321.
23. PADDISON, P.J., CAUDY, A.A., BERNSTEIN, E., HANNON, G.J., and CONKLIN, D.S. (2002a). Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev. 16, 948-958.
24. PADDISON, P.J., CAUDY, A.A., and HANNON, G.J. (2002b). Stable suppression of gene expression by RNAi in mammalian cells. Proc. Natl. Acad. Sci. USA 99, 1443-1448.
25. PADDISON, P.J., and HANNON, G.J. (2002). RNA interference: The new somatic cell genetics? Cancer Cell 2, 17-23.
26. PARRISH, S., FLEENOR, J., XU, S.Q., MELLO, C., and FIRE, A. (2000). Functional anatomy of a dsRNA trigger: Differential requirement for the two trigger strands in RNA interference. Mol. Cell 6, 1077-1087.
27. PAUL, C.P., GOOD, P.D., WINER, I., and ENGELKE, D.R. (2002). Effective expression of small interfering RNA in human cells. Nat. Biotechnol. 20, 505-508.
28. SAMBROOK, J., and RUSSEL, D.W. (2002). Molecular Cloning: A Laboratory Manual, 3 ed. (Cold Spring Harbor Press, Cold Spring, NY).
29. SCADDEN, A.D.J., and SMITH, C.W.J. (2001). RNAi is antagonized by A ≥ I hyper-editing. EMBO Rep. 2, 1107-1111.
30. SCHEPERS, U., GLOMBITZA, G., LEMM, T., HOFFMANN, A., CHABAS, A., OZAND, P., and SANDHOFF, K. (1996). Molecular analysis of a Gm2-activator deficiency in two patients with Gm2-gangliosidosis Ab variant. Am. J. Hum. Genet. 59, 1048-1056.
31. SCHEPERS, U., and KOLTER, T. (2001). RNA interference: A new way to analyze protein function. Angew. Chem. Int. Ed. 40, 2437-2439.
32. SCHUETTE, C.G., PIERSTORFF, B., HUETTLER, S., and SANDHOFF, K. (2001). Sphingolipid activator proteins: Proteins with complex functions in lipid degradation and skin biogenesis. Glycobiology 11, 81R-90R.
33. SLEDZ, C.A., HOLKO, M., DE VEER, M.J., SILVERMAN, R.H., and WILLIAMS, B.R.G. (2003). Activation of the interferon system by short-interfering RNAs. Nat. Cell Biol. 5, 834-839.
34. SMITH, N.A., SINGH, S.P., WANG, M.B., STOUTJEDIJK, P.A., GREEN, A.G., and WATERHOUSE, P.M. (2000). Total silencing by intron spliced hairpin RNAs. Nature 407, 319-320.
35. SPRONG, H., DEGROOTE, S., CLAESSENS, T., VAN DRUNEN, J., OORSCHOT, V., WESTERINK, B.H., HIRABAYASHI, Y., KLUMPERMAN, J., VAN DER SLUIJS, P., and VAN MEER, G. (2001). Glycosphingolipids are required for sorting melanosomal proteins in the Golgi complex. J. Cell Biol. 155, 369-380.
36. SUI, G., SOOHOO, C., AFFAR EL, B., GAY, F., SHI, Y., and FORRESTER, W.C. (2002). A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc. Natl. Acad. Sci. USA 99, 5515-5520.
37. SVOBODA, P., STEIN, P., HAYASHI, H., and SCHULTZ, R.M. (2000). Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference. Development 127, 4147-4156.
38. SVOBODA, P., STEIN, P., and SCHULTZ, R.M. (2001). RNAi in mouse oocytes and preimplantation embryos: Effectiveness of hairpin dsRNA. Biochem. Biophys. Res. Commun. 287, 1099-1104.
39. TUSCHL, T., ZAMORE, P.D., LEHMANN, R., BARTEL, D.P., and SHARP, P.A. (1999). Targeted mRNA degradation by double-stranded RNA in vitro. Genes Dev. 13, 3191-3197.
40. VAN ECHTEN-DECKERT, G. (2000). Sphingolipid extraction and analysis by thin layer chromatography. Methods Enzymol. 312, 64-79.
41. WIANNY, F., and ZERNICKA-GOETZ, M. (2000). Specific interference with gene function by double-stranded RNA in early mouse development. Nat. Cell Biol. 2, 70-75.
42. YANG, D., LU, H., and ERICKSON, J.W. (2000). Evidence that processed small dsRNAs may mediate sequence-specific mRNA degradation during RNAi in Drosophila embryos. Curr. Biol. 10, 1191-1200.
43. YU, J.Y., DERUITER, S.L., and TURNER, D.L. (2002). RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc. Natl. Acad. Sci. USA 99, 6047-6052.
44. ZAMORE, P.D. (2001). RNA interference: Listening to the sound of silence. Nat. Struct. Biol. 8, 746-750.