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An excellent review on mRNA localization with a focus on molecular motors involved in localization and on Drosophila mRNA localization.
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Tekotte H., Davis I. Intracellular mRNA localization: motors move messages. Trends Genet. 18:2002;636-642 An excellent review on mRNA localization with a focus on molecular motors involved in localization and on Drosophila mRNA localization.
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Up-to-date and short summary on methods and approaches in RNA live imaging.
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Proposes a new function for a kinesin motor in posterior RNA localization in Drosophila oocytes. Kinesin is required to avoid unwanted cortical attachment of localized mRNAs rather than to direct movement.
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Cha B.-J., Serbus L.R., Koppetsch B.S., Theurkauf W.E. Kinesin I-dependent cortical exclusion restricts pole plasm to the oocyte posterior. Nat. Cell. Biol. 4:2002;592-598 Proposes a new function for a kinesin motor in posterior RNA localization in Drosophila oocytes. Kinesin is required to avoid unwanted cortical attachment of localized mRNAs rather than to direct movement.
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••] present compelling evidence for cytoplasmic dynein having a function in anterior RNA localization. In addition, a model of how kinesin might recycle dynein for further transport rounds is proposed.
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Evidence for an RNA cargo having a regulatory influence on transport kinetics. Possible explanations for this phenomenon include control of motor efficiency by the RNA cargo.
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Bullock S.L., Zicha D., Ish-Horowicz D. The Drosophila hairy RNA localization signal modulates the kinetics of cytoplasmic mRNA transport. EMBO J. 22:2003;2484-2494 Evidence for an RNA cargo having a regulatory influence on transport kinetics. Possible explanations for this phenomenon include control of motor efficiency by the RNA cargo.
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••] are promising biochemical purification approaches to isolating and characterizing localizing RNPs from neurons and Drosophila embryos. Both groups identified motor proteins co-purifying with their respective RNPs.
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