A uniform procedure for the purification of CDK7/CycH/MAT1, CDK8/CycC and CDK9/CycT1

Biological Procedures Online

Volumn 6, Issue 1, 2004, Pages 163-172

  Pinhero, Reena ^a   Liaw, Peter ^a   Yankulov, Krassimir ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

Baculoviridae; Cyclin dependent kinases; Isolation and purification

Indexed keywords

CYCLIN DEPENDENT KINASE 7; CYCLIN DEPENDENT KINASE 8; CYCLIN DEPENDENT KINASE 9; HISTIDINE; NICKEL; RECOMBINANT ENZYME;

ANIMAL CELL; ARTICLE; CHROMATOGRAPHY; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PHOSPHORYLATION; ENZYME PURIFICATION; ENZYME SUBUNIT; INSECT CELL; LEPIDOPTERA; NONHUMAN; PROTEIN EXPRESSION; STANDARDIZATION;

BACULOVIRIDAE; INSECTA; SPODOPTERA FRUGIPERDA;

EID: 16344365524     PISSN: 14809222     EISSN: None     Source Type: Journal    
DOI: 10.1251/bpo86     Document Type: Article

References (30)

1. Morgan DO. Cyclin-dependent kinases: engines, clocks, and microprocessors. The dynamics of cyclin dependent kinase structure. Annu Rev Cell Dev Biol 1997; 13(6):261-291.
2. Kobor MS, Greenblatt J. Regulation of transcription elongation by phosphorylation. Biochim Biophys Acta 2002; 1577(2):261-275.
3. Palancade B, Bensaude O. Investigating RNA polymerase II carboxyl-terminal domain (CTD) phosphorylation. Eur J Biochem 2003; 270(19):3859-3870.
4. Ramanathan YS, Rajpara M, Reza SM, Lees E, Shuman S, Mathews MB, Pe'ery T. Three RNA polymerase II carboxylterminal domain kinases display distinct substrate preferences. J Biol Chem 2001; 276(14):10913-10920.
5. Rickert P, Corden JL, Lees E. Cyclin C/CDK8 and cyclin H/CDK7/p36 are biochemically distinct CTD kinases. Oncogene 1999; 18(4):1093-1102.
6. Price DH. P-TEFb, a cyclin-dependent kinase controlling elongation by RNA polymerase II. Mol Cell Biol 2000; 20(8):2629-2634.
7. Ping YH, Rana TM. Tat-associated kinase (P-TEFb): a component of transcription preinitiation and elongation complexes. J Biol Chem 1999; 274(11):7399-7404.
8. Nigg EA. Cyclin-dependent kinase-7 - at the cross-roads of transcription, DNA-repair and cell-cycle control. Curr Opin Cell Bio 1996; 8(3):312-317.
9. Rochette-Egly C, Adam S, Rossignol M, Egly JM, Chambon P. Stimulation of RAR alpha activation function AF-1 through binding to the general transcription factor TFIIH and phosphorylation by CDK7. Cell 1997; 90(1):97-107.
10. Bastien J, Adam-Stitah S, Riedl T, Egly JM, Chambon P, Rochette-Egly C. TFIIH interacts with the retinoic acid receptor gamma and phosphorylates its AF-1-activating domain through cdk7. J Biol Chem 2000; 275(29):21896-21904.
11. Chen D, Riedl T, Washbrook E, Pace PE, Coombes RC, Egly JM, Ali S. Activation of estrogen receptor alpha by S118 phosphorylation involves a ligand-dependent interaction with TFIIH and participation of CDK7. Mol Cell 2000; 6(1):127-137.
12. Inamoto S, Segil N, Pan ZQ, Kimura M, Roeder RG. The cyclin-dependent kinase-activating kinase (CAK) assembly factor, MAT1, targets and enhances CAK activity on the POU domains of octamer transcription factors. J Biol Chem 1997; 272(47):29852-29858.
13. Ko LJ, Shieh SY, Chen X, Jayaraman L, Tamai K, Taya Y, Prives C, Pan ZQ. p53 is phosphorylated by CDK7-cyclin H in a p36MAT1-dependent manner. Mol Cell Biol 1997; 17(21):7220-7229.
14. Pinhero R, Liaw P, Bertens K, Yankulov K. Three cyclindependent kinases preferentially phosphorylate different parts of the C-terminal domain of the large subunit of RNA polymerase II. Eur J Biochem 2004; 271(5):1004-1014.
15. Peng J, Zhu Y, Milton JT, Price DH. Identification of multiple cyclin subunits of human P-TEFb. Genes Dev 1998; 12(5):755-762.
16. Richardson CD, Banville M, Lalumiere M, Vialard J, Meighen EA. Bacterial luciferase produced with rapidscreening baculovirus vectors is a sensitive reporter for infection of insect cells and larvae. Intervirology 1992; 34(4):213-227.
17. Fisher R, Jin P, Chamberlin H, Morgan D. Alternative mechanisms of CAK assembly require an assembly factor or an activating kinase. Cell 1995; 83(1):47-57.
18. Yankulov KY, Bentley DL. Regulation of CDK7 substrate specificity by MAT1 and TFIIH. Embo J 1997; 16(7):1638-1646.
19. Kikkawa U, Minakuchi R, Takai Y, Nishizuka Y. Calciumactivated, phospholipid-dependent protein kinase (protein kinase C) from rat brain. Methods Enzymol 1983; 99:288-298.
20. Rossignol M, Egly JM. Substrate specificity of the cdkactivating kinase (CAK) is altered upon association with TFIIH. Embo J 1997; 16(7):1628-1637.
21. Fisher RP, Morgan DO. A novel cyclin associates with MO15/CDK7 to form the CDK-activating kinase. Cell 1994; 78(4):713-724.
22. Larochelle S, Chen J, Knights R, Pandur J, Morcillo P, Erdjument-Bromage H, Tempst P, Suter B, Fisher RP. Tloop phosphorylation stabilizes the CDK7-cyclin H-MAT1 complex in vivo and regulates its CTD kinase activity. Embo J 2001; 20(14):3749-3759.
23. Ljungman M, Paulsen MT. The cyclin-dependent kinase inhibitor roscovitine inhibits RNA synthesis and triggers nuclear accumulation of p53 that is unmodified at Ser15 and Lys382. Mol Pharmacol 2001; 60(4):785-789.
24. Schang LM. Cyclin-dependent kinases as cellular targets for antiviral drugs. J Antimicrob Chemother 2002; 50(6):779-792.
25. Taylor SL, Kinchington PR, Brooks A, Moffat JF. Roscovitine, a cyclin-dependent kinase inhibitor, prevents replication of varicella-zoster virus. J Virol 2004; 78(6):2853-2862.
26. Sano M, Abdellatif M, Oh H, Xie M, Bagella L, Giordano A, Michael LH, DeMayo FJ, Schneider MD. Activation and function of cyclin T-Cdk9 (positive transcription elongation factor-b) in cardiac muscle-cell hypertrophy. Nat Med 2002; 8(11):1310-1317.
27. Zhou M, Halanski MA, Radonovich MF, Kashanchi F, Peng J, Price DH, Brady JN. Tat modifies the activity of CDK9 to phosphorylate serine 5 of the RNA polymerase II carboxylterminal domain during human immunodeficiency virus type 1 transcription. Mol Cell Biol 2000; 20(14):5077-5086.
28. Dubois MF, Vincent M, Vigneron M, Adamczewski J, Egly JM, Bensaude O. Heat-shock inactivation of the tfiihassociated kinase and change in the phosphorylation sites on the c-terminal domain of RNA-polymerase-II. Nucleic Acids Research 1997; 25:694-700.
29. Yee A, Wu L, Liu L, Kobayashi R, Xiong Y, Hall FL. Biochemical characterization of the human cyclin-dependent protein kinase activating kinase. J Biol Chem 1996; 271(1):471-477.
30. Adamczewski JP, Rossingol M, Tassan JP, Nigg EA, Moncollin V, Egly JM. Mat1, cdk7 and cyclin-H form a kinase complex which is uv light-sensitive upon association with TFIIH. Embo J 1996; 15(8):1877-1884.