메뉴 건너뛰기




Volumn 15, Issue 4, 2005, Pages 1149-1154

Carbonic anhydrase inhibitors. Inhibition of the membrane-bound human and bovine isozymes IV with sulfonamides

Author keywords

[No Author keywords available]

Indexed keywords

ACETAZOLAMIDE; BROMOSULFANILAMIDE; CARBONATE DEHYDRATASE I; CARBONATE DEHYDRATASE II; CARBONATE DEHYDRATASE INHIBITOR; CARBONATE DEHYDRATASE IV; CELECOXIB; DICLOFENAMIDE; DORZOLAMIDE; ETHOXZOLAMIDE; METHAZOLAMIDE; SULFANILAMIDE DERIVATIVE; SULFONAMIDE; TOPIRAMATE; UNCLASSIFIED DRUG; VALDECOXIB;

EID: 16244381734     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2004.12.009     Document Type: Article
Times cited : (31)

References (39)
  • 6
    • 2442579987 scopus 로고    scopus 로고
    • Carbonic Anhydrases: Catalytic Mechanism, Distribution and Physiological Roles
    • C.T. Supuran A. Scozzafava J. Conway CRC Boca Raton (FL), USA
    • C.T. Supuran Carbonic Anhydrases: Catalytic Mechanism, Distribution and Physiological Roles C.T. Supuran A. Scozzafava J. Conway Carbonic Anhydrase-Its Inhibitors and Activators 2004 CRC Boca Raton (FL), USA 1 24
    • (2004) Carbonic Anhydrase-Its Inhibitors and Activators , pp. 1-24
    • Supuran, C.T.1
  • 34
    • 85030817244 scopus 로고    scopus 로고
    • note
    • 4, 300 mM NaCl, 10 mM imidazole, pH 8.0) per gram of cells containing 200 μg/mL lysozyme. The suspension was incubated on ice for 30 min and treated with a sonicator (six times for 10 s with 10 s cooling time in between). After centrifugation at 10,000g for 30 min at 4°C the cleared lysate was incubated with Ni-NTA suspension (Qiagen). The expressed protein was purified according to the manufacturer's protocol. The isolated hCA IV was further purified by Sephadex G25 chromatography (Amersham). The purified enzyme had a catalytic activity very similar to that described in Ref. 8
  • 35
    • 0015239422 scopus 로고
    • 2 solutions in water at 20°C were used as substrate. Stock solutions of inhibitors were prepared at a concentration of 10-50 mM (in the assay buffer) and dilutions up to 0.01 nM done with the assay buffer mentioned above. Enzyme concentrations were 0.09 μM for hCA I, 0.06 μM for hCA II, 1 μM for hCA IV and bCA IV. Inhibition constants were calculated as described by R.G. Khalifah J. Biol. Chem. 246 1971 2561 2573
    • (1971) J. Biol. Chem. , vol.246 , pp. 2561-2573
    • Khalifah, R.G.1
  • 36
    • 2442650952 scopus 로고    scopus 로고
    • Clinical Applications of Carbonic Anhydrase Inhibitors in Ophthalmology
    • V.T. Supuran A. Scozzafava J. Conway CRC Boca Raton, FL
    • F. Mincione, L. Menabuoni, and C.T. Supuran Clinical Applications of Carbonic Anhydrase Inhibitors in Ophthalmology V.T. Supuran A. Scozzafava J. Conway Carbonic Anhydrase: Its Inhibitors and Activators 2004 CRC Boca Raton, FL 243 254
    • (2004) Carbonic Anhydrase: Its Inhibitors and Activators , pp. 243-254
    • Mincione, F.1    Menabuoni, L.2    Supuran, C.T.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.