Purification, characterization, and sequence analysis of two α-amylase isoforms from azuki bean, vigna angularis, showing different affinity towards β-cyclodextrin sepharose

Bioscience, Biotechnology and Biochemistry

Volumn 67, Issue 5, 2003, Pages 1080-1093

  Mar, San San ^a   Mori, Haruhide ^a   Lee, Jin Ha ^a   Fukuda, Kenji ^a   Saburi, Wataru ^a   Fukuhara, Arinobu ^a   Okuyama, Masayuki ^a   Chiba, Seiya ^a   Kimura, Atsuo ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Alternative splicing; Azuki bean amylase; Carbohydrate binding site; Isoforms; Possible posttranslational modification

Indexed keywords

AMYLASE; BETA CYCLODEXTRIN; BETA CYCLODEXTRIN DERIVATIVE; CALCIUM; CHELATING AGENT; CYCLODEXTRIN; EDETIC ACID; ISOENZYME; PLANT EXTRACT; SEPHAROSE; STARCH; TRYPSIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; HYDROLYSIS; ISOLATION AND PURIFICATION; KINETICS; LEGUME; MASS SPECTROMETRY; MOLECULAR GENETICS; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN PROCESSING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TEMPERATURE;

ALPHA-AMYLASE; AMINO ACID SEQUENCE; BETA-CYCLODEXTRINS; CALCIUM; CHELATING AGENTS; CHROMATOGRAPHY, AFFINITY; CYCLODEXTRINS; EDETIC ACID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FABACEAE; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; ISOENZYMES; KINETICS; MOLECULAR SEQUENCE DATA; PLANT EXTRACTS; PROTEIN PROCESSING, POST-TRANSLATIONAL; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEPHAROSE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STARCH; SUBSTRATE SPECIFICITY; TEMPERATURE; TRYPSIN;

FELIS CATUS; PHASEOLUS (ANGIOSPERM); VIGNA; VIGNA ANGULARIS;

EID: 1542755136     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.67.1080     Document Type: Article

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