Modulation of activity and substrate binding modes by mutation of single and double subsites +1/+2 and -5/-6 of barley α-amylase 1

European Journal of Biochemistry

Volumn 268, Issue 24, 2001, Pages 6545-6558

  Mori, Haruhide ^a   Bak Jensen, Kristian Sass ^a   Gottschalk, Tine E ^a   Motawia, Mohammed Saddik ^b   Damager, Iben ^b   Møller, Birger Lindberg ^b   Svensson, Birte ^a  

^a CARLSBERG LABORATORY   (Denmark)

^b UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

( )8 barrel; Branched malto oligosaccharide.; Glycoside hydrolase family 13; Site directed mutagenesis; Substrate specificity

Indexed keywords

ACARBOSE; AMINO ACID; AMYLASE; AMYLOSE; GLYCOGEN; HEPTOSE; HEXOSE; MALTOSE; MUTANT PROTEIN; OLIGOSACCHARIDE; STARCH;

AMINO ACID SUBSTITUTION; ANALYTIC METHOD; ARTICLE; BARLEY; BINDING SITE; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME REGULATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; MUTATION; PRIORITY JOURNAL;

ALPHA-AMYLASE; AMYLOSE; BASE SEQUENCE; DNA PRIMERS; GLYCOGEN; HORDEUM; HYDROLYSIS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; OLIGOSACCHARIDES; PROTEIN BINDING; SUBSTRATE SPECIFICITY;

HORDEUM VULGARE SUBSP. VULGARE;

EID: 0035660447     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02609.x     Document Type: Article

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