Probing the precision of the mitotic clock with a live-cell fluorescent biosensor

Nature Biotechnology

Volumn 22, Issue 3, 2004, Pages 306-312

  Jones, Joshua T ^a   Myers, Jason W ^a   Ferrell Jr , James E ^a   Meyer, Tobias ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSENSORS; CELLS; FLUORESCENCE; OPTICAL MICROSCOPY;

NUCLEAR ENVELOPE BREAKDOWN (NEB); TOTAL INTERNAL REFLECTION FLUORESCENCE (TIRF) MICROSCOPY;

BIOTECHNOLOGY;

PACLITAXEL; PROTEIN MAD2; RNA;

ACCURACY; ANIMAL CELL; ARTICLE; BIOSENSOR; CELL; CELL DIVISION; CELL NUCLEUS MEMBRANE; CELL NUCLEUS MEMBRANE BREAKDOWN; CHRONOBIOLOGY; FLUORESCENCE; FLUORESCENCE MICROSCOPY; LEUKEMIA CELL; LIVE CELL; LIVE CELL FLUORESCENT BIOSENSOR; MEMBRANE DAMAGE; MEMBRANE RECONSTITUTION; MITOSIS; MITOSIS SPINDLE; MITOTIC CLOCK; NONHUMAN; PRIORITY JOURNAL; RAT; TOTAL INTERNAL FLUORESCENCE MICROSCOPY;

ANIMALS; BIOSENSING TECHNIQUES; CELL CULTURE TECHNIQUES; CELL LINE; CELL NUCLEUS; EQUIPMENT DESIGN; EQUIPMENT FAILURE ANALYSIS; IMAGE INTERPRETATION, COMPUTER-ASSISTED; MICROSCOPY, FLUORESCENCE; MICROSCOPY, VIDEO; MITOSIS; RATS;

ANIMALIA;

EID: 1542276726     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt941     Document Type: Article

References (27)

1. Ekholm, S.V., Zickert, P., Reed, S.I. & Zetterberg, A. Accumulation of cyclin E is not a prerequisite for passage through the restriction point. Mol. Cell. Biol. 21, 3256-3265 (2001).
2. Elledge, S.J. Cell cycle checkpoints: preventing an identity crisis. Science 274, 1664-1672 (1996).
3. Musacchio, A. & Hardwick, K.G. The spindle checkpoint: structural insights into dynamic signalling. Nature Rev. Mol. Cell Biol. 3, 731-741 (2002).
4. Yu, H. Regulation of APC-Cdc20 by the spindle checkpoint. Curr. Opin. Cell Biol. 14, 706-714 (2002).
5. Michel, L.S. et al. MAD2 haplo-insufficiency causes premature anaphase and chromosome instability in mammalian cells. Nature 409, 355-359 (2001).
6. Rieder, C.L. & Khodjakov, A. Mitosis through the microscope: advances in seeing inside live dividing cells. Science 300, 91-96 (2003).
7. Bajer, A. Cine-Micrographic Studies on mitosis in endosperm. Exp. Cell Res. 14, 245-256 (1958).
8. Rieder, C.L. Effect of hypothermia (20-25 degrees C) on mitosis in PtK1 cells. Cell Biol. Int. Rep. 5, 563-573 (1981).
9. Zhang, J., Campbell, R.E., Ting, A.Y. & Tsien, R.Y. Creating new fluorescent probes for cell biology. Nat. Rev. Mol. Cell Biol. 3, 906-918 (2002).
10. Steyer, J.A. & Almers, W. A real-time view of life within 100 nm of the plasma membrane. Nat. Rev. Mol. Cell Biol. 2, 268-275 (2001).
11. Tengholm, A., Teruel, M.N. & Meyer, T. Single cell imaging of PI3K activity and glucose transporter insertion into the plasma membrane by dual color evanescent wave microscopy. Science's Stke 2003, PL4 (2003).
12. Haraguchi, T. et al. Live fluorescence imaging reveals early recruitment of emerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopes. J. Cell Sci. 113, 779-794 (2000).
13. Wang, T.H., Wang, H.S. & Soong, Y.K. Paclitaxel-induced cell death: where the cell cycle and apoptosis come together. Cancer 88, 2619-2628 (2000).
14. Jordan, M.A., Toso, R.J., Thrower, D. & Wilson, L. Mechanism of mitotic block and inhibition of cell proliferation by taxol at low concentrations. Proc. Nat. Acad. Sci. USA 90, 9552-9556 (1993).
15. Jordan, M.A. et al. Mitotic block induced in HeLa cells by low concentrations of paclitaxel (Taxol) results in abnormal mitotic exit and apoptotic cell death. Cancer Res. 56, 816-825 (1996).
16. Sorger, P.K., Dobles, M., Tournebize, R. & Hyman, A.A. Coupling cell division and cell death to microtubule dynamics. Curr. Opin. Cell Biol. 9, 807-814 (1997).
17. Rieder, C.L., Schultz, A., Cole, R.W. & Sluder, G. Anaphase onset in vertebrate somatic cells is controlled by a checkpoint that monitors sister kinetochore attachment to the spindle. J. Cell Biol. 127, 1301-1310 (1994).
18. Gorbsky, G.J., Chen, R.H. & Murray, A.W. Microinjection of antibody to Mad2 protein into mammalian cells in mitosis induces premature anaphase. J. Cell Biol. 141, 1193-1205 (1998).
19. Fang, G., Yu, H. & Kirschner, M.W. The checkpoint protein MAD2 and the mitotic regulator CDC20 form a ternary complex with the anaphase-promoting complex to control anaphase initiation. Genes Dev. 12, 1871-1883 (1998).
20. Dobles, M., Liberal, V., Scott, M.L., Benezra, R. & Sorger, P.K. Chromosome missegregation and apoptosis in mice lacking the mitotic checkpoint protein Mad2. Cell 101, 635-645 (2000).
21. Mikhailov, A., Cole, R.W. & Rieder, C.L. DNA damage during mitosis in human cells delays the metaphase/anaphase transition via the spindle-assembly checkpoint. Curr. Biol. 12, 1797-1806 (2002).
22. Canman, J.C., Salmon, E.D. & Fang, G. Inducing precocious anaphase in cultured mammalian cells. Cell Mol. Cyto. 52, 61-65 (2002).
23. Myers, J.W., Jones, J.T., Meyer, T. & Ferrell, J.E. Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing. Nat. Biotechnol. 21, 324-328 (2003).
24. Teruel, M.N. & Meyer, T. Parallel single-cell monitoring of receptor-triggered membrane translocation of a calcium-sensing protein module. Science 295, 1910-1912 (2002).
25. Heo, W.D. & Meyer, T. Switch-of-function mutants based on morphology classification of ras superfamily small GTPases. Cell 113, 315-328 (2003).
26. Campbell, R.E. et al. A monomeric red fluorescent protein. Proc. Nat. Acad. Sci. USA 99, 7877-7882 (2002).
27. Rekas, A., Alattia, J.R., Nagai, T., Miyawaki, A. & Ikura, M. Crystal structure of venus, a yellow fluorescent protein with improved maturation and reduced environmental sensitivity. J. Biol. Chem. 277, 50573-50578 (2002).