Development of a fluorescent reporter to assess iron regulatory protein activity in living cells

Biochimica et Biophysica Acta - Molecular Cell Research

Volumn 1743, Issue 1-2, 2005, Pages 162-168

  Henderson, Rebecca J ^a   Patton, Stephanie M ^a   Connor, James R ^a  

^a PENN STATE COLLEGE OF MEDICINE   (United States)

Author keywords

Ferritin; Fluorescent reporter; IRE; Iron regulation

Indexed keywords

CYAN FLUORESCENT PROTEIN; FERRITIN; FLUORESCENT DYE; IRON; IRON REGULATORY FACTOR; MESSENGER RNA;

ARTICLE; CELL STRAIN HEK293; EMBRYO; EXPRESSION VECTOR; GENE INSERTION; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; IRON KINETICS; IRON RESPONSIVE ELEMENT; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN SYNTHESIS; REPORTER GENE;

EID: 15044365381     PISSN: 01674889     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbamcr.2004.09.032     Document Type: Article

References (39)

1. D.J. Pinero, J. Hu, and J.R. Connor Alterations in the interaction between iron regulatory proteins and their iron responsive element in normal and Alzheimer's diseased brains Cell. Mol. Biol. 46 2000 761 776 (Noisy-le-grand)
2. J.R. Connor Metals and Oxidative Damge in Neurological Disorders 1997 Plenum Press New York
3. S.H. Itzkowitz, and X. Yio Inflammation and Cancer IV. Colorectal cancer in inflammatory bowel disease: the role of inflammation Am. J. Physiol. Gastrointest. Liver Physiol. 287 2004 G7 G17
4. J.A. Gomez Vitamin E attenuates biochemical and morphological features associated with development of chronic pancreatitis Am. J. Physiol. Gastrointest. Liver Physiol. 287 2004 G162 G169
5. D. Girelli Hereditary hyperferritinemia-cataract syndrome caused by a 29-base pair deletion in the iron responsive element of ferritin L-subunit gene Blood 90 1997 2084 2088
6. R. Eisenstein Iron regulatory proteins and the molecular control of mammalian iron metabolism Annu. Rev. Nutr. 20 2000 627 662
7. R.S. Eisenstein, and K.P. Blemings Iron regulatory proteins, iron responsive elements and iron homeostasis J. Nutr. 128 1998 2295 2298
8. R.S. Eisenstein Iron-responsive element-binding protein. Phosphorylation by protein kinase C J. Biol. Chem. 268 1993 27363 27370
9. M.A. Garate, and M.T. Nunez Overexpression of the ferritin iron-responsive element decreases the labile iron pool and abolishes the regulation of iron absorption by intestinal epithelial (Caco-2) cells J. Biol. Chem. 275 2000 1651 1655
10. S.W. Hulet Characterization and distribution of ferritin binding sites in the adult mouse brain J. Neurochem. 72 1999 868 874
11. D.J. Pinero Interleukin-1beta increases binding of the iron regulatory protein and the synthesis of ferritin by increasing the labile iron pool Biochim. Biophys. Acta 1497 2000 279 288
12. K.J. Thompson, S. Shoham, and J.R. Connor Iron and neurodegenerative disorders Brain Res. Bull. 55 2001 155 164
13. Technical Assistance Group, M.P., "Calcein Longevity." R.J. Henderson, 2004.
14. R.P. Haugland Fluorescent Indicators for Zn(2+) and Other Metal Ions J. Gregory Handbook of Fluorescent Probes and Research Products 2002 Molecular Probes Eugene, OR 805 816
15. F.J. O'Brien, D. Taylor, and T.C. Lee An improved labelling technique for monitoring microcrack growth in compact bone J. Biomech. 35 2002 523 526
16. E. Uchimura In-situ visualization and quantification of mineralization of cultured osteogenetic cells Calcif. Tissue Int. 73 2003 575 583
17. L.V. Hale, Y.F. Ma, and R.F. Santerre Semi-quantitative fluorescence analysis of calcein binding as a measurement of in vitro mineralization Calcif. Tissue Int. 67 2000 80 84
18. 2+-permeable AMPA/kainate receptor activation in murine neocortical neurons Neurosci. Lett. 351 2003 145 148
19. J.R. Forbes, and P. Gros Iron, manganese, and cobalt transport by Nramp1 (Slc11a1) and Nramp2 (Slc11a2) expressed at the plasma membrane Blood 102 2003 1884 1892
20. R.M. Andersson Modulation of Na+,K+-ATPase activity is of importance for RVD Acta Physiol. Scand. 180 2004 329 334
21. E. Paraskeva Ribosomal pausing and scanning arrest as mechanisms of translational regulation from cap-distal iron-responsive elements Mol. Cell. Biol. 19 1999 807 816
22. L.S. Goessling Irreversible steps in the ferritin synthesis induction pathway J. Biol. Chem. 269 1994 4343 4348
23. M. Goralska The effect of ascorbic acid and ferric ammonium citrate on iron uptake and storage in lens epithelial cells Exp. Eye Res. 66 1998 687 697
24. J. Butt Differences in the RNA binding sites of iron regulatory proteins and potential target diversity Proc. Natl. Acad. Sci. U. S. A. 93 1996 4345 4349
25. Y.H. Wang, S.R. Sczekan, and E.C. Theil Structure of the 5′ untranslated regulatory region of ferritin mRNA studied in solution Nucleic Acids Res. 18 1990 4463 4468
26. E.C. Theil Iron regulatory elements (IREs): a family of mRNA non-coding sequences J. Biochem. 304 Pt 1 1994 1 11
27. E.C. Theil, R.A. McKenzie, and H. Sierzputowska-Gracz Structure and function of IREs, the noncoding mRNA sequences regulating synthesis of ferritin, transferrin receptor and (erythroid) 5-aminolevulinate synthase Adv. Exp. Med. Biol. 356 1994 111 118
28. D.J. Pinero The intracellular location of iron regulatory proteins is altered as a function of iron status in cell cultures and rat brain J. Nutr. 131 2001 2831 2836
29. E.E. Cable, and H.C. Isom Metabolism of 3,5,5-trimethylhexanoyl-ferrocene by rat liver: release of iron from 3,5,5-trimethylhexanoyl-ferrocene by a microsomal, phenobarbital-inducible cytochrome P-450 Drug Metab. Dispos. 27 1999 255 260
30. E.E. Cable, J.R. Connor, and H.C. Isom Accumulation of iron by primary rat hepatocytes in long-term culture: changes in nuclear shape mediated by non-transferrin-bound forms of iron Am. J. Pathol. 152 1998 781 792
31. A.C. Chua Multidentate pyridinones inhibit the metabolism of nontransferrin-bound iron by hepatocytes and hepatoma cells Eur. J. Biochem. 270 2003 1689 1698
32. H.N. Traore, and D. Meyer Comparing qualitative and quantitative spectroscopic techniques for the detection of the effect of direct iron loading of mammalian cell cultures Methods Cell Sci. 23 2001 175 184
33. K. Bittman, "Mean Pixel Density Analysis of ImagePro Software for Fluorescent Quantitation." 2004, Phase 3 Imaging: Glen Mills, PA.
34. D.R. Richardson Iron and gallium increase iron uptake from transferrin by human melanoma cells: further examination of the ferric ammonium citrate-activated iron uptake process Biochim. Biophys. Acta 1536 2001 43 54
35. J. Pines GFP in mammalian cells Trends Genet. 11 1995 326 327
36. K.R. Siemering Mutations that suppress the thermosensitivity of green fluorescent protein Curr. Biol. 6 1996 1653 1663
37. B. Goetze Coupling the iron-responsive element to GFP-an inducible system to study translation in a single living cell Sci. STKE 2003 2003 PL12
38. P. Macchi A GFP-based system to uncouple mRNA transport from translation in a single living neuron Mol. Biol. Cell 14 2003 1570 1582
39. X.B. Liu, P. Hill, and D.J. Haile Role of the ferroportin iron-responsive element in iron and nitric oxide dependent gene regulation Blood Cells Mol. Diseases 29 2002 315 326