Accumulation of iron by primary rat hepatocytes in long-term culture: Changes in nuclear shape mediated by non-transferrin-bound forms of iron

American Journal of Pathology

Volumn 152, Issue 3, 1998, Pages 781-792

  Cable, Edward E ^a   Connor, James R ^a   Isom, Harriet C ^a  

^a PENN STATE MILTON S HERSHEY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DICIFERRON; FERRITIN; FERROCENE DERIVATIVE; FERROUS SULFATE; HEMOSIDERIN; IRON; IRON DERIVATIVE; NITRILOTRIACETATE IRON; TRANSFERRIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL NUCLEUS; CELL SHAPE; CONTROLLED STUDY; IRON OVERLOAD; LIVER CELL CULTURE; MALE; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALS; CELL NUCLEUS; CELL SIZE; CELLS, CULTURED; FERRIC COMPOUNDS; FERRITINS; FERROUS COMPOUNDS; HEMOSIDERIN; HISTOCYTOCHEMISTRY; IRON; LIVER; LYSOSOMES; MALE; MICROSCOPY, ELECTRON; NITRILOTRIACETIC ACID; RATS; RATS, INBRED F344; TRANSFERRIN;

EID: 0031911846     PISSN: 00029440     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (59)

1. Harford JB, Rouault TA Huebers HA, Klausner RD: Molecular mechanisms of iron metabolism. The Molecular Basis of Blood Diseases. Edited by Stamatoyannopoulos G, Nienhuis AW, Majerus PW, Varmus H. Philadelphia, WB Saunders, 1994, pp 351-297
2. Cook JD: Adaptation in iron metabolism. Am J Clin Nutr 1990, 51: 301-308
3. Martinez-Torres C, Leets I, Taylor P, Ramirez J, del Valle Camacho M, Layrisse M: Heme, ferritin, and vegetable iron absorption in humans from meals denatured of heme iron during the cooking of beef. J Nutr 1986, 116:1720-1725
4. Young SP, Aisen P: The liver and iron. The Liver: Biology and Pathobiology. Edited by Arias IM, Boyer JL, Fausto N, Jakoby WB. New York, Raven Press, 1994, pp 597-617
5. Lash A, Saleem A: Iron metabolism and its regulation: a review. Ann Clin Lab Sci 1995, 25:20-30
6. Lynch SR, Skikne BS, Cook JD: Food iron absorption in idiopathic hemochromatosis. Blood 1989, 74:2187-2193
7. Nielsen P, Heinrich HC: Metabolism of iron from (3,5,5-trimethylhexanoyl)ferrocene in rats: a dietary model for severe iron overload. Biochem Pharmacol 1993, 45:385-391
8. Britton RS, O'Neill R, Bacon BR: Hepatic mitochondrial malondialdehyde metabolism in rats with chronic iron overload. Hepatology 1990, 11:93-97
9. Bacon BR, Healey JF, Brittenham GM, Park CH, Nunnari J, Tavill AS, Bonkovsky HL: Hepatic microsomal function in rats with chronic dietary iron overload. Gastroenterology 1986, 90:1844-1853
10. Düllmann J, Wulfhekel U, Nielsen P, Heinrich HC: Iron overload of the liver by trimethylhexanoylferrocene in rats. Acta Anat 1992, 143:96-108
11. Longueville A, Crichton RC: An animal model of iron overload and its application to study hepatic ferritin iron mobilisation by chelators. Biochem Pharmacol 1986, 35:3669-3678
12. Bothwell TH, Charlton RW, Motulsky AG: Hemochromatosis. The Metabolic and Molecular Basis of Inherited Disease. Edited by Scriver CR, Beaudet AL, Sly WS, Valle D. New York, McGraw-Hill, 1995, pp 2237-2269
13. Grootveld M, Bell JD, Halliwell B, Aruoma OI, Bomford A, Sadler PJ: Non-transferrin-bound iron in plasma or serum from patients with idiopathic hemochromatosis: characterization by high performance liquid chromatography and nuclear magnetic resonance spectrosoopy. J Biol Chem 1989, 264:4417-4422
14. Stremmel W, Riedel HD, Niederau C, Strohmeyer G: Pathogenesis of genetic haemochromatosis. Eur J Clin Invest 1993, 23:321-329
15. Stohs SJ, Bagchi D: Oxidative mechanisms in the toxicity of metal ions. Free Radical Biol Med 1995, 18:321-336
16. Brissot P, Wright TL, Ma WL, Weisiger RA: Efficient clearance of non-transferrin-bound iron by rat liver: implications for hepatic iron loading in iron overload states. J Clin Invest 1985, 76:1463-1470
17. Kennard ML, Richardson DR, Gabathuler R, Ponka P, Jefferies WA: A novel iron uptake mechanism mediated by GPI-anchored human p97. EMBO J 1995, 14:4178-4186
18. m transporter for non-transferrin-bound iron in isolated rat hepatocytes. Am J Physiol 1995, 269:G570-G576
19. Nielsen P, Düllmann J, Wulfhekel U, Heinrich HC: Non-transferrin-bound-iron in serum and low-molecular-weight-iron in the liver of dietary iron-loaded rats. Int J Biochem 1993, 25:223-232
20. Isom H, Georgoff I, Salditt-Georgieff M, Darnell JE Jr: Persistence of liver-specific messenger RNA in cultured hepatocytes: different regulatory events for different genes. J Cell Biol 1987, 105:2877-2885
21. Isom HC, Secott T, Georgoff I, Woodworth C, Mummaw J: Maintenance of differentiated rat hepatocytes in primary culture. Proc Natl Acad Sci USA 1985, 82:3252-3256
22. Hu JM, Camper SA, Tilghman SM, Miller T, Georgoff I, Serra R, Isom HC: Functional analyses of albumin expression in a series of hepatocyte cell lines and in primary hepatocytes. Cell Growth Differ 1992, 3:577-588
23. Woodworth CD, Isom HC: Transformation of differentiated rat hepatocytes with adenovirus and adenovirus DNA. J Virol 1987, 61:3570-3579
24. Woodworth CD, Isom HC: Regulation of albumin gene expression in a series of rat hepatocyte cell lines immortalized by simian virus 40 and maintained in chemically defined medium. Mol Cell Biol 1987, 7:3740-3748
25. Woodworth C, Secott T, Isom HC: Transformation of rat hepatocytes by transfection with simian virus 40 DNA to yield proliferating differentiated cells. Cancer Res 1986, 46:4018-4026
26. Serra R, Isom HC: Stimulation of DNA synthesis and protooncogene expression in primary rat hepatocytes in long-term DMSO culture. J Cell Physiol 1993, 154:543-553
27. Cable EE, Isom HC: Exposure of primary rat hepatocytes in long-term DMSO culture to selected transition metals induces hepatocyte proliferation and formation of duct-like structures. Hepatology 1997, 26: 1444-1457
28. Isom HC: DNA synthesis in isolated hepatocytes infected with herpesviruses. Virology 1980, 103:199-216
29. Feldhoff RC, Taylor JM, Jefferson LS: Synthesis and secretion of rat albumin in vivo, in perfused liver, and in isolated hepatocytes. J Biol Chem 1977, 11853:29211-11385
30. Berry MN, Friend DS: High-yield preparation of isolated rat parenchymal cells. J Cell Biol 1969, 43:506-520
31. Elsdale T, Bard J: Collagen substrata for studies on cell behavior. J Cell Biol 1972, 54:626-637
32. Scheuer PJ, Lefkowitch JH: Laboratory Techniques: Liver Biopsy Interpretation. London, WB Saunders, 1994, pp 10-15
33. Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC: Measurement of protein using bicinchoninic acid. Anal Biochem 1985, 150: 76-85
34. Mohler JL, Partin AW, Lohr WD, Coffey DS: Nuclear roundness factor measurement for assessment of prognosis of patients with prostatic carcinoma. I. Testing of a digitization system. J Urol 1988, 139:1080-1084
35. Diamond DA, Berry SJ, Umbricht C, Jewett HJ, Coffey DS: Computerized image analysis of nuclear shape as a prognostic factor for prostatic cancer. Prostate 1982, 3:321-332
36. Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685
37. Wydner KS, Godyn JJ, Lee ML, Sciorra LJ: A new approach to the computer-assisted quantitative analysis of nuclear shape. Mod Pathol 1991, 4:154-160
38. Batey RG, Lai Chung Fong P, Shamir S, Sherlock S: A non-transferrin-bound serum iron in idiopathic hemochromatosis. Dig Dis Sci 1980, 25:340-346
39. Araujo A, Kosaryan M, MacDowell A, Wickens D, Puri S, Wonke B, Hoffbrand AV: A novel delivery system for continuous desferrioxamine infusion in transfusional iron overload. Br J Haematol 1996, 93: 835-837
40. Wang WC, Ahrned N, Hanna M: Non-transferrin-bound iron in longterm transfusion in children with congenital anemias. J Pediatr 1986, 108:552-557
41. Al-Refaie FN, Wickens DG, Wonke B, Kontoghiorghes GJ, Hoffbrand AV: Serum non-transferrin-bound iron in β-thalassaemia major patients treated with desferrioxamine and L1. Br J Haematol 1992, 82:431-436
42. Wright TL, Brissot P, Ma WL, Weisiger RA: Characterization of non-transferrin-bound iron clearance by rat liver. J Biol Chem 1986, 261: 10909-10914
43. 2+ antiport. Biochim Biophys Acta 1996, 1282:163-170
44. Qian ZM, Morgan EH: Changes in the uptake of transferrin-free and transferrin-bound iron during reticulocyte maturation in vivo and in vitro. Biochim Biophys Acta 1992, 1135:35-43
45. Porter JB, Abeysinghe RD, Marshall L, Hider RC, Singh S: Kinetics of removal and reappearance of non-transferrin-bound plasma iron with deferoxamine therapy. Blood 1996, 88:705-713
46. Batey RG, Shamir S, Wilms J: Properties and hepatic metabolism of non-transferrin-bound iron. Dig Dis Sci 1981, 26:1084-1088
47. Jacobs A: Low molecular weight intracellular iron transport compounds. Blood 1977, 50:433-439
48. Britton RS, Bacon BR, Recknagel RO: Lipid peroxidation and associated hepatic organelle dysfunction in iron overload. Chem Phys Lipids 1987, 45:207-239
49. Britton RS: Metal-induced hepatotoxicity. Semin Liver Dis 1996, 16:3-12
50. Casey JL, Koeller DM, Ramin VC, Klausner RD, Harford JB: Iron regulation of transferrin receptor mRNA levels requires iron-responsive elements and a rapid turnover determinant in the 3′ untranslated region of the mRNA. EMBO J 1989, 8:3693-3699
51. Harford JB, Klausner RD: Coordinate post transcriptional regulation of ferritin and transferrin receptor expression: the role of regulated RNA-protein interaction. Enzyme 1990, 44:28-41
52. Trinder D, Batey RG, Morgan EH, Baker E: Effect of cellular iron concentration on iron uptake by hepatocytes. Am J Physiol 1990, 259:G611-G617
53. Müller-Eberhard U, Liem HH, Grasso JA, Giffhorn-Katz S, DeFalco MG, Katz NR: Increase in surface expression of transferrin receptors on cultured hepatocytes of adult rats in response to iron deficiency. J Biol Chem 1988, 263:14753-14756
54. Ingber DE, Dike L, Hansen L, Karp S, Liley H, Maniotis A, McNamee H, Mooney D, Plopper G, Sims J, Wang N: Cellular tensegrity: exploring how mechanical changes in the cytoskeleton regulate cell growth, migration, and tissue pattern during morphogenesis. Int Rev Cytol 1994, 150:173-224
55. Sims JR, Karp S, Ingber DE: Altering the cellular mechanical force balance results in integrated changes in cell, cytoskeletal and nuclear shape. J Cell Sci 1992, 103:1215-1222
56. Pool TB, Heitman TO, Buck MA: Changes in nuclear shape and mitochondrial structure do not accompany the loss of division potential in human fibroblasts in vitro. Am J Anat 1981, 162:369-382
57. Karim OM, Seki N, Pienta KJ, Mostwin JL: The effect of age on the response of the detrusor to intracellular mechanical stimulus: DNA replication and the cell actin matrix. J Cell Biochem 1992, 48:373-384
58. Bour ES, Ward LK, Cornman GA, Isom HC: Tumor necrosis factor-α-induced apoptosis in hepatocytes in long-term culture. Am J Pathol 1996, 148:485-495
59. Cammisa HM, Isom HC, Greene FE: Hormonal regulation of pseudocholinesterase activity in cultured rat hepatocytes. Endocrinology 1988, 122:991-996