A comparison of the effects of betaine and S-adenosylmethionine on ethanol-induced changes in methionine metabolism and steatosis in rat hepatocytes

Journal of Nutrition

Volumn 135, Issue 3, 2005, Pages 519-524

  Kharbanda, Kusum K ^a,b   Rogers II, David D ^a   Mailliard, Mark E ^a,b   Siford, Gerri L ^b   Barak, Anthony J ^a,b   Beckenhauer, Harriet C ^a   Sorrell, Michael F ^a,b   Tuma, Dean J ^a,b  

^a VA Alcohol Research Center   (United States)

^b UNIVERSITY OF NEBRASKA MEDICAL CENTER   (United States)

Author keywords

Betaine; Ethanol; Hepatocytes; S adenosylhomocysteine; S adenosylmethionine

Indexed keywords

ALCOHOL; BETAINE; HOMOCYSTEINE; METHIONINE; S ADENOSYLHOMOCYSTEINE; S ADENOSYLMETHIONINE; TRIACYLGLYCEROL;

ALCOHOL CONSUMPTION; AMINO ACID METABOLISM; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; DRUG EFFICACY; FATTY LIVER; LIVER CELL; LIVER METABOLISM; MALE; METHYLATION; NONHUMAN; RAT; RAT STRAIN;

RATTUS NORVEGICUS;

EID: 14944373556     PISSN: 00223166     EISSN: None     Source Type: Journal    
DOI: 10.1093/jn/135.3.519     Document Type: Article

References (43)

1. Barak, A. J., Beckenhauer, H. C., Tuma, D. J. & Badakhsh, S. (1987) Effects of prolonged ethanol feeding on methionine metabolism in rat liver. Biochem. Cell Biol. 65: 230-233.
2. Finkelstein, J. D. (1990) Methionine metabolism in mammals. J. Nutr. Biochem. 1: 228-237.
3. Tsukamoto, H. & Lu, S. C. (2001) Current concepts in the pathogenesis of alcoholic liver injury. FASEB J. 15: 1335-1349.
4. Barak, A. J., Beckenhauer, H. C. & Tuma, D. J. (1985) Ethanol feeding inhibits the activity of N5-methyltetrahydrofolate-homocysteine methyltransferase in the rat. IRCS Med. Sci. 13: 760-761.
5. Halsted, C. H., Villanueva, J., Chandler, C. J., Stabler, S. P., Allen, R. H., Muskhelishvili, L., James, S. J. & Poirier, L. (1996) Ethanol feeding of micropigs alters methionine metabolism and increases hepatocellular apoptosis and proliferation. Hepatology 23: 497-505.
6. Barak, A. J., Beckenhauer, H. C. & Tuma, D. J. (2002) Methionine synthase: a possible prime site of the ethanolic lesion in liver. Alcohol 26: 65-67.
7. Barak, A. J., Beckenhauer, H. C., Kharbanda, K. K. & Tuma, D. J. (2001) Chronic ethanol consumption increases homocysteine accumulation in hepatocytes. Alcohol 25: 77-81.
8. Lieber, C. S., Casini, A., DeCarli, L. M., Kim, C. I., Lowe, N., Sasaki, R. & Leo, M. A. (1990) S-Adenosyl-L-methionine attenuates alcohol-induced liver injury in the baboon. Hepatology 11: 165-172.
9. Halsted, C. H., Villanueva, J. A., Devlin, A. M., Niemela, O., Parkkila, S., Garrow, T. A., Wallock, L. M., Shigenaga, M. K., Melnyk, S. & James, S. J. (2002) Folate deficiency disturbs hepatic methionine metabolism and promotes liver injury in the ethanol-fed micropig. Proc. Natl. Acad. Sci. U.S.A. 99: 10072-10077.
10. Ji, C. & Kaplowitz, N. (2003) Betaine decreases hyperhomocysteinemia, endoplasmic reticulum stress, and liver injury in alcohol-fed mice. Gastroenterology 124: 1488-1499.
11. Barak, A. J., Beckenhauer, H. C., Mailliard, M. E., Kharbanda, K. K. & Tuma, D. J. (2003) Betaine lowers elevated S-adenosylhomocysteine levels in hepatocytes from ethanol-fed rats. J. Nutr. 133: 2845-2848.
12. Hoffman, D. R., Haning, J. A. & Cornatzer, W. E. (1981) Microsomal phosphatidylethanolamine methyltransferase: inhibition by S- adenosylhomocysteine. Lipids 16: 561-567.
13. Noga, A. A., Zhao, Y. & Vance, D. E. (2002) An unexpected requirement for phosphatidylethanolamine N-methyltransferase in the secretion of very low density lipoproteins. J. Biol. Chem. 277: 42358-42365.
14. Barak, A. J., Beckenhauer, H. C., Junnila, M. & Tuma, D. J. (1993) Dietary betaine promotes generation of hepatic S-adenosylmethionine and protects the liver from ethanol-induced fatty infiltration. Alcohol. Clin. Exp. Res. 17:552-555.
15. Barak, A. J., Beckenhauer, H. C. & Tuma, D. J. (1994) S-Adenosylmethionine generation and prevention of alcoholic fatty liver by betaine. Alcohol 11: 501-503.
16. Kanbak, G., Inal, M. & Baycu, C. (2001) Ethanol-induced hepatotoxicity and protective effect of betaine. Cell Biochem. Funct. 19: 281-285.
17. Barak, A. J., Beckenhauer, H. C., Badakhsh, S. & Tuma, D. J. (1997) The effect of betaine in reversing alcoholic steatosis. Alcohol. Clin. Exp. Res. 21:1100-1102.
18. Lieber, C. S. (2002) S-Adenosyl-L-methionine: its role in the treatment of liver disorders. Am. J. Clin. Nutr. 76: 1183S-1187S.
19. Lieber, C. S. & DeCarli, L. M. (1989) Liquid diet technique of ethanol administration: 1989 update. Alcohol Alcohol 24: 197-211.
20. (1996) Guide for the Care and Use of Laboratory Animals. National Academy Press, Washington, DC.
21. Casey, C. A., Kragskow, S. L., Sorrell, M. F. & Tuma, D. J. (1987) Chronic ethanol administration impairs the binding and endocytosis of asialoorosomucoid in isolated rat hepatocytes. J. Biol. Chem. 262: 2704-2710.
22. Graf, D., Kurz, A. K., Reinehr, R., Fischer, R., Kircheis, G. & Haussinger, D. (2002) Prevention of bile acid-induced apoptosis by betaine in rat liver. Hepatology 36: 829-839.
23. Yang, H., Sadda, M. R., Li, M., Zeng, Y., Chen, L., Bae, W., Ou, X., Runnegar, M. T., Mato, J. M. & Lu, S. C. (2004) S-Adenosylmethionine and its metabolite induce apoptosis in HepG2 cells: role of protein phosphatase 1 and Bcl-x(S). Hepatology 40: 221-231.
24. Cornwell, P. E., Morgan, S. L. & Vaughn, W. H. (1993) Modification of a high-performance liquid chromatographic method for assay of homocysteine in human plasma. J. Chromatogr. 617: 136-139.
25. Fu, W., Dudman, N. P., Perry, M. A., Young, K. & Wang, X. L. (2000) Interrelations between plasma homocysteine and intracellular S-adenosylhomocysteine. Biochem. Biophys. Res. Commun. 271: 47-53.
26. Folch, J., Lees, M. & Sloan-Stanley, G. H. (1957) A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226: 497-509.
27. Clarke, S. & Banfield, K. (2001) S-Adenosylmethionine-dependent methyltransferases. In: Homocysteine in Health and Disease (Carmel, R. & Jacobsen, D. W., eds.), pp. 63-78. Cambridge University Press, Cambridge, MA.
28. Refsum, H., Fiskerstrand, T., Guttormsen, A. B. & Ueland, P. M. (1997) Assessment of homocysteine status. J. Inherit. Metab. Dis. 20: 286-294.
29. Colell, A., Garcia-Ruiz, C., Morales, A., Ballesta, A., Ookhtens, M., Rodes, J., Kaplowitz, N. & Fernandez-Checa, J. (1997) Transport of reduced glutathione in hepatic mitochondria and mitoplasts from ethanol-treated rats: effect of membrane physical properties and S-adenosyl-L-methionine. Hepatology 26:699-708.
30. Garcia-Ruiz, C., Morales, A., Colell, A., Ballesta, A., Rodes, J., Kaplowitz, N. & Fernandez-Checa, J. (1995) Feeding S-adenosyl-L-methionine attenuates both ethanol induced depletion of mitochondrial glutathione and mitochondrial dysfunction in periportal and perivenous rat hepatocytes. Hepatology 21:207-214.
31. Friedel, H. A., Goa, K. L. & Benfield, P. (1989) S-Adenosyl-L- methionine. A review of its pharmacological properties and therapeutic potential in liver dysfunction and affective disorders in relation to its physiological role in cell metabolism. Drugs 38: 389-416.
32. Mato, J. M., Camara, J., Fernandez de Paz, J., Caballeria, L., Coll, S., Caballero, A., Garcia-Buey, L., Beltran, J., Benita, V., Caballeria, J., Sola, R., Moreno-Otero, R., Barrao, F., Martin-Duce, A., Correa, J. A., Pares, A., Barrao, E., Garcia-Magaz, I., Puerta, J. L., Moreno, J., Boissard, G., Ortiz, P. & Rodes, J. (1999) S-Adenosylmethionine in alcoholic liver cirrhosis: a randomized, placebo-controlled, double-blind, multicenter clinical trial. J. Hepatol. 30: 1081-1089.
33. Zhu, X., Song, J., Mar, M. H., Edwards, L. J. & Zeisel, S. H. (2003) Phosphatidylethanolamine-N-methyltransferase (PEMT) knockout mice have hepatic steatosis and abnormal hepatic choline metabolite concentrations despite ingesting a recommended dietary intake of choline. Biochem. J. 370: 987-993.
34. Selhub, J. (1999) Homocysteine metabolism. Annu. Rev. Nutr. 19:217-246.
35. Hofmann, M. A., Lalla, E., Lu, Y., Gleason, M. R., Wolf, B. M., Tanji, N., Ferran, L. J., Jr., Kohl, B., Rao, V., Kisiel, W., Stern, D. M. & Schmidt, A. M. (2001) Hyperhomocysteinemia enhances vascular inflammation and accelerates atherosclerosis in a murine model. J. Clin. Investig. 107: 675-683.
36. Kang, S. S., Wong, P. W. & Malinow, M. R. (1992) Hyperhomocyst(e) inemia as a risk factor for occlusive vascular disease. Annu. Rev. Nutr. 12: 279-298.
37. Li, N., Yi, F. X., Rute, E., Zhang, D. X., Slocum, G. R. & Zou, A. P. (2002) Effects of homocysteine on intracellular nitric oxide and superoxide levels in the renal arterial endothelium. Am. J. Physiol. 283: H1237-H1243.
38. Majors, A. K., Sengupta, S., Jacobsen, D. W. & Pyeritz, R. E. (2002) Upregulation of smooth muscle cell collagen production by homocysteine- insight into the pathogenesis of homocystinuria. Mol. Genet. Metab. 76: 92-99.
39. Majors, A. K., Sengupta, S., Willard, B., Kinter, M. T., Pyeritz, R. E. & Jacobsen, D. W. (2002) Homocysteine binds to human plasma fibronectin and inhibits its interaction with fibrin. Arterioscler. Thromb. Vasc. Biol. 22: 1354-1359.
40. Maler, J. M., Seifert, W., Huther, G., Wiltfang, J., Ruther, E., Kornhuber, J. & Bleich, S. (2003) Homocysteine induces cell death of rat astrocytes in vitro. Neurosci. Lett. 347: 85-88.
41. Miller, J. W., Green, R., Mungas, D. M., Reed, B. R. & Jagust, W. J. (2002) Homocysteine, vitamin B6, and vascular disease in AD patients. Neurology 58: 1471-1475.
42. Refsum, H., Ueland, P. M., Nygard, O. & Vollset, S. E. (1998) Homocysteine and cardiovascular disease. Annu. Rev. Med. 49: 31-62.
43. Torres, L., Garcia-Trevijano, E. R., Rodriguez, J. A., Carretero, M. V., Bustos, M., Fernandez, E., Eguinoa, E., Mato, J. M. & Avila, M. A. (1999) Induction of TIMP-1 expression in rat hepatic stellate cells and hepatocytes: a new role for homocysteine in liver fibrosis. Biochim. Biophys. Acta 1455: 12-22.