Amino-terminal protein fusions to the TraR quorum-sensing transcription factor enhance protein stability and autoinducer-independent activity

Journal of Bacteriology

Volumn 187, Issue 4, 2005, Pages 1219-1226

  Chai, Yunrong ^a   Winans, Stephen C ^a  

^a Department of Molecular Biology and Genetics   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

3 OXOOCTANOYLHOMOSERINE LACTONE; ACYLTRANSFERASE; AMINOGLYCOSIDE N ACETYLTRANSFERASE; HOMOSERINE; HYBRID PROTEIN; LACTONE DERIVATIVE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR LUXR; TRANSCRIPTION FACTOR TRAR; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANTIBIOTIC RESISTANCE; ARTICLE; CELL COMMUNICATION; CONTROLLED STUDY; ESCHERICHIA COLI; GEL MOBILITY SHIFT ASSAY; IMMUNOPRECIPITATION; NONHUMAN; PLASMID; POINT MUTATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN FOLDING; PROTEIN STABILITY; QUORUM SENSING; RHIZOBIUM RADIOBACTER; WESTERN BLOTTING;

ACETYLTRANSFERASES; BACTERIAL PROTEINS; DNA MUTATIONAL ANALYSIS; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; HOMOSERINE; MUTATION; PLASMIDS; RECOMBINANT FUSION PROTEINS;

EID: 14544280599     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.187.4.1219-1226.2005     Document Type: Article

References (43)

1. Akakura, R., and S. C. Winans. 2002. Constitutive mutations of the OccR regulatory protein affect DNA bending in response to metabolites released from plant tumors. J. Biol. Chem. 277:5866-5874.
2. Cangelosi, G. A., E. A. Best, G. Martinetti, and E. W. Nester. 1991. Genetic analysis of agrobacterium. Methods Enzymol. 204:384-397.
3. Chai, Y., and S. C. Winans. 2004. Site-directed mutagenesis of a LuxR-type quorum-sensing transcription factor: alteration of autoinducer specificity. Mol. Microbiol. 51:765-776.
4. Chai, Y., J. Zhu, and S. C. Winans. 2001. TrlR, a defective TraR-like protein of Agrobacterium tumefaciens, blocks TraR function in vitro by forming inactive TrlR: TraR dimers. Mol. Microbiol. 40:414-421.
5. Cho, K., C. Fuqua, B. Martin, and S. Winans. 1996. Identification of Agrobacterium tumefaciens genes that direct the complete catabolism of octopine. J. Bacteriol. 178:1872-1880.
6. Choi, S. H., and E. P. Greenberg. 1991. The C-terminal region of the Vibrio fischeri LuxR protein contains an inducer-independent lux gene activating domain. Proc. Natl. Acad. Sci. USA 88:11115-11119.
7. Choi, S. H., and E. P. Greenberg. 1992. Genetic dissection of DNA binding and luminescence gene activation by the Vibrio fischeri LuxR protein. J. Bacteriol. 174:4064-4069.
8. Egland, K. A., and E. P. Greenberg. 2001. Quorum sensing in Vibrio fischeri: analysis of the LuxR DNA binding region by alanine-scanning mutagenesis. J. Bacteriol. 183:382-386.
9. Fox, J. B., and D. S. Waugh. 2003. Maltose-binding protein as a solubility enhancer. Methods Mol. Biol. 205:99-117.
10. Fuqua, C., M. R. Parsek, and E. P. Greenberg. 2001. Regulation of gene expression by cell-to-cell communication: acyl-homoserine lactone quorum sensing. Annu. Rev. Genet. 35:439-468.
11. Fuqua, W. C., and S. C. Winans. 1994. A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumor metabolite. J. Bacteriol. 176:2796-2806.
12. Gegg, C. V., K. E. Bowers, and C. R. Matthews. 1997. Probing minimal independent folding units in dihydrofolate reductase by molecular dissection. Protein Sci. 6:1885-1892.
13. Hajdukiewicz, P., Z. Svab, and P. Maliga. 1994. The small, versatile pPZP family of Agrobacterium binary vectors for plant transformation. Plant Mol. Biol. 25:989-994.
14. Hanzelka, B. L., and E. P. Greenberg. 1995. Evidence that the N-terminal region of the Vibrio fischeri LuxR protein constitutes an autoinducer-binding domain. J. Bacteriol. 177:815-817.
15. Harlow, E., and D. Lane. 1988. Antibodies: a laboratory manual, p. 457-470. Cold Spring Harbor Laboratory Press, Plainview, N.Y.
16. Kiratisin, P., K. D. Tucker, and L. Passador. 2002. LasR, a transcriptional activator of Pseudomonas aeruginosa virulence genes, functions as a multimet. J. Bacteriol. 184:4912-4919.
17. LaVallie, E. R., E. A. DiBlasio-Smith, L. A. Collins-Racie, Z. Lu, and J. M. McCoy. 2002. Thioredoxin and related proteins as multifunctional fusion tags for soluble expression in E. coli. Methods Mol. Biol, 205:119-140.
18. Luo, Z. Q., A. J. Smyth, P. Gao, Y. Qin, and S. K. Farrand, 2003. Mutational analysis of TraR. Correlating function with molecular structure of a quorumsensing transcriptional activator. J. Biol. Chem. 278:13173-13182.
19. Ma, B., C.-J. Tsai, and R. Nussinov. 2000. Binding and folding: in search of intramolecular chaperone-like building block fragments. Protein Eng. 13: 617-627.
20. Miller, J. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
21. Miller, M. B., and B. L. Bassler. 2001. Quorum sensing in bacteria. Annu. Rev. Microbiol. 55:165-199.
22. Nakamura, T., and M. Iwakura. 1999. Circular permutation analysis as a method for distinction of functional elements in the M20 loop of Escherichia coli dihydrofolate reductase. J. Biol. Chem. 274:19041-19047.
23. Pelletier, J. N., K. M. Arndt, A. Pluckthun, and S. W. Michnick. 1999. An in vivo library-versus-library selection of optimized protein-protein interactions. Nat. Biotechnol. 17:683-690.
24. Pelletier, J. N., F.-X. Campbell-Valois, and S. W. Michnick. 1998. Oligomerization domain-directed reassembly of active dihydrofolate reductase from rationally designed fragments. Proc. Natl. Acad. Sci. USA 95:12141-12146.
25. Poellinger, K. A., J. P. Lee, J. V. Parales, Jr., and E. P. Greenberg. 1995. Intragenic suppression of a luxR mutation: characterization of an autoinducer-independent LuxR. FEMS Microbiol. Lett. 129:97-101.
26. Qin, Y., Z. Q. Luo, A. J. Smyth, P. Gao, S. Beck von Bodman, and S. K. Farrand. 2000. Quorum-sensing signal binding results in dimerization of TraR and its release from membranes into the cytoplasm. EMBO J. 19:5212-5221.
27. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
28. Schaefer, A. L., B. L. Hanzelka, A. Eberhard, and E. P. Greenberg. 1996. Quorum sensing in Vibrio fischeri: probing autoinducer-LuxR interactions with autoinducer analogs. J. Bacteriol. 178:2897-2901.
29. Sham, Y. Y., B. Ma, C.-J. Tsai, and R. Nussinov. 2001. Molecular dynamics simulation of Escherichia coli dihydrofolate reductase and its protein fragments: relative stabilities in experiment and simulations. Protein Sci. 10:135-148.
30. Shaw, K. J., P, N. Rather, R. S. Hare, and G. H. Miller. 1993. Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes. Microbiol. Rev. 57:138-163.
31. Sitnikov, D. M., G. S. Shadel, and T. O. Baldwin. 1996. Autoinducer-independent mutants of the LuxR transcriptional activator exhibit differential effects on the two lux promoters of Vibrio fischeri. Mol. Gen. Genet. 252: 622-662.
32. Urbanowski, M. L., C. P. Lostroh, and E. P. Greenberg. 2004. Reversible acyl-homoserine lactone binding to purified Vibrio fischeri LuxR protein. J. Bacteriol. 186:631-637.
33. Vannini, A., C. Volpari, C. Gargioli, E. Muraglia, R. Cortese, R. De Francesco, P. Neddermann, and S. D. Marco. 2002. The crystal structure of the quorum sensing protein TraR bound to its autoinducer and target DNA. EMBO J. 21:4393-4401.
34. Weingart, C. L., C. E. White, S. Liu, Y. Chai, H. Cho, C.-S. Tsai, Y. Wei, N. R. Delay, A. Eberhard, and S. C. Winans. Submitted for publication,
35. Whitehead, N. A., A. M. Barnard, H. Slater, N. J. Simpson, and G. P. Salmond. 2001. Quorum-sensing in Gram-negative bacteria. FEMS Microbiol. Rev. 25:365-404.
36. Winans, S. C., and B. L. Bassler. 2002. Mob psychology. J. Bacteriol. 184: 873-883.
37. Wolf, E., A. Vassilev, Y. Makino, A. Sali, Y. Nakatani, and S. K. Burley. 1998. Crystal structure of a GCN5-related N-acetyltransferase: Serratia marcescens aminoglycoside 3-N-acetyltransferase. Cell 94:439-449.
38. Yabuta, Y., E. Subbian, C. Olry, and U. Shinde. 2003. Folding pathway mediated by an intramolecular chaperone. J. Biol. Chem. 278:15246-15251.
39. Zhang, R. G., T. Pappas, J. L. Brace, P. C. Miller, T. Oulmassov, J. M. Molyneaux, J. C. Anderson, J. K. Bashkin, S. C. Winans, and A. Joachimiak. 2002. Structure of a bacterial quorum-sensing transcription factor complexed with pheromone and DNA. Nature 417:971-974.
40. Zhu, J., J. W. Beaber, M. I. More, C. Fuqua, A. Eberhard, and S. C. Winans. 1998. Analogs of the autoinducer 3-oxooctanoyl-homoserine lactone strongly inhibit activity of the TraR protein of Agrobacterium tumefaciens. J. Bacteriol. 180:5398-5405.
41. Zhu, J., and S. C. Winans. 1998. Activity of the quorum-sensing regulator TraR of Agrobacterium tumefaciens is inhibited by a truncated, dominant defective TraR-like protein. Mol. Microbiol. 27:289-297.
42. Zhu, J., and S. C. Winans. 1999. Autoinducer binding by the quorum-sensing regulator TraR increases affinity for target promoters in vitro and decreases TraR turnover rates in whole cells. Proc. Natl. Acad. Sci. USA 96:4832-4837.
43. Zhu, J., and S. C. Winans. 2001. The quorum-sensing transcriptional regulator TraR requires its cognate signaling ligand for protein folding, protease resistance, and dimerization. Proc. Natl. Acad. Sci. USA 98:1507-1512.