Autoinducer-independent mutants of the LuxR transcriptional activator exhibit differential effects on the two lux promoters of Vibrio fischeri

Molecular and General Genetics

Volumn 252, Issue 5, 1996, Pages 622-625

  Sitnikov, Dmitry M ^a   Shadel, Gerald S ^a,b   Baldwin, Thomas O ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

^b STANFORD UNIVERSITY   (United States)

Author keywords

Autoinducer; Luminescence; LuxR; Quorum sensing; Vibrio fischeri

Indexed keywords

ARTICLE; BACTERIUM MUTANT; BINDING SITE; BIOLUMINESCENCE; CONTROLLED STUDY; GENE CLUSTER; GENE EXPRESSION REGULATION; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN CONFORMATION; TRANSCRIPTION INITIATION; VIBRIO;

4-BUTYROLACTONE; BACTERIAL PROTEINS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; HYDROXYLAMINE; HYDROXYLAMINES; LUMINESCENT MEASUREMENTS; MUTAGENESIS; MUTATION; OPERON; PHENOTYPE; PLASMIDS; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; REPRESSOR PROTEINS; TRANS-ACTIVATION (GENETICS); TRANS-ACTIVATORS; VIBRIO;

BACTERIA (MICROORGANISMS); VIBRIO FISCHERI;

EID: 0029958050     PISSN: 00268925     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380050269     Document Type: Article

References (9)

1. Baldwin TO, Devine JH, Heckel RC, Lin JW, Shadel GS (1989) The complete nucleotide sequence of the lux regulon of Vibrio fischeri and the luxABN region of Photobacterium leiognathi and the mechanism of control of bacterial bioluminescenee. J Biolum Chemilum 4:326-341
2. Devine JH (1993) The LuxR transcriptional activator from Vibrio fischeri strain ATCC 7744: studies of the LuxR protein and its interaction with the luxICDABEG promoter. Ph.D. thesis, Texas A&M University, College Station, TX 77843
3. Dunlap PV, Greenberg EP (1988) Control of Vibrio fischeri lux gene transcription by a cyclic AMP receptor protein-LuxR protein regulatory circuit. J Bacteriol 170:4040-4046
4. Fuqua WC, Winans SC, Greenberg EP (1994) Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators. J Bacteriol 176:269-275
5. Meighen EA, Dunlap PV (1993) Physiological, biochemical and genetic control of Bacterial Bioluminescence. Adv Microb Physiol 34:1-67
6. Shadel GS, Baldwin TO (1991) The Vibrio fischeri LuxR protein is capable of bidirectional stimulation of transcription and both positive and negative regulation of luxR. J Bacteriol 173:568-574
7. Shadel GS, Baldwin TO (1992) Identification of a distantly located regulatory element in the luxD gene required for negative autoregulation of the Vibrio fischeri luxR gene. J Biol Chem 267:7690-7695
8. Shadel GS, Young R, Baldwin TO (1990) Use of regulated cell lysis in a lethal genetic selection in Escherichia coli: identification of the autoinducer-binding region of the LuxR protein from Vibrio fischeri ATCC 7744. J Bacteriol 172:3980-3987
9. Sitnikov DM, Schineller JB, Baldwin TO (1995) Transcriptional regulation of bioluminescence genes from Vibrio fischeri. Mol Microbiol 17:801-812