Simple method for determination of the number of Helicobacter pylori CagA variable-region EPIYA tyrosine phosphorylation motifs by PCR

Journal of Clinical Microbiology

Volumn 43, Issue 2, 2005, Pages 791-795

  Argent, Richard H ^a,b   Zhang, Youli ^c   Atherton, John C ^a  

^a Inst Infect Immun Inflammation   (United Kingdom)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^c AFFILIATED HOSPITAL OF JIANGSU UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CAGA PROTEIN;

ARTICLE; CELL STRUCTURE; CONTROLLED STUDY; EPITHELIUM CELL; GENE AMPLIFICATION; GENE TRANSLOCATION; GENETIC VARIABILITY; HELICOBACTER PYLORI; INTERMETHOD COMPARISON; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENICITY ISLAND; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN MOTIF; PROTEIN PHOSPHORYLATION; SEQUENCE ANALYSIS; STOMACH CARCINOMA; STOMACH EPITHELIUM; STRAIN DIFFERENCE;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; HELICOBACTER PYLORI; HUMANS; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; POLYMERASE CHAIN REACTION; REPETITIVE SEQUENCES, NUCLEIC ACID; TYROSINE; VARIATION (GENETICS);

HELICOBACTER PYLORI;

EID: 13844271835     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.43.2.791-795.2005     Document Type: Article

References (33)

1. Akopyants, N. S., S. W. Clifton, D. Kersulyte, J. E. Crabtree, B. E. Youree, C. A. Reece, N. O. Bukanov, E. S. Drazek, B. A. Roe, and D. E. Berg. 1998. Analyses of the cag pathogenicity island of Helicobacter pylori. Mol. Microbiol. 28:37-53.
2. Alm, R. A., L.-S. L. Ling, D. T. Moir, B. L. King, E. D. Brown, P. C. Doig, D. R. Smith, B. Noonan, B. C. Guild, B. L. deJonge, G. Carmel, P. J. Tummino, A. Caruso, M. Uria-Nickelsen, D. M. Mills, C. Ives, R. Gibson, D. Merberg, S. D. Mills, Q. Jiang, D. E. Taylor, G. F. Vovis, and T. J. Trust. 1999. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature 397:176-180.
3. Argent, R. H., M. Kidd, R. J. Owen, R. J. Thomas, M. C. Limb, and J. C. Atherton. 2004. Determinants and consequences of different levels of CagA phosphorylation for clinical isolates of Helicobacter pylori. Gastroenterology 127:514-523.
4. Asahi, M., T. Azuma, S. Ito, Y. Ito, H. Suto, Y. Nagai, M. Tsubokawa, Y. Tohyama, S. Maeda, M. Omata, T. Suzuki, and C. Sasakawa. 2000. Helicobacter pylori CagA protein can be tyrosine phosphorylated in gastric epithelial cells. J. Exp. Med. 191:593-602.
5. Asahi, M., Y. Tanaka, T. Izumi, Y. Ito, H. Naiki, D. Kersulyte, K. Tsujikawa, M. Saito, K. Sada, S. Yanagi, A. Fujikawa, M. Noda, and Y. Itokawa. 2003. Helicobacter pylori CagA containing ITAM-like sequences localized to lipid rafts negatively regulates VacA-induced signaling in vivo. Helicobacter 8:1-14.
6. Atherton, J. C. 1997. Molecular methods for detecting ulcerogenic strains of H. pylori, p. 133-143. In C. L. Clayton and H. L. T. Mobley (ed.), Methods in molecular medicine, Helicobacter pylori protocols. Humana Press, Totowa, N.J.
7. Azuma, T., A. Yamakawa, S. Yamazaki, K. Fukuta, M. Ohtani, Y. Ito, M. Dojo, Y. Yamazaki, and M. Kuriyama. 2002. Correlation between variation of the 3′ region of the cagA gene in Helicobacter pylori and disease outcome in Japan. J. Infect. Dis. 186:1621-1630.
8. Azuma, T., S. Yamazaki, A. Yamakawa, M. Ohtani, A. Muramatsu, H. Suto, Y. Ito, M. Dojo, Y. Yamazaki, Y. Keida, H. Higashi, and M. Hatakeyama. 2004. Association between diversity in the Src homology 2 domain-containing tyrosine phosphatase binding site of Helicobacter pylori CagA protein and gastric atrophy and cancer. J. Infect. Dis. 189:820-827.
9. Backert, S., S. Moese, M. Selbach, V. Brinkmann, and T. F. Meyer. 2001. Phosphorylation of tyrosine 972 of the Helicobacter pylori CagA protein is essential for induction of a scattering phenotype in gastric epithelial cells. Mol. Microbiol. 42:631-644.
10. Backert, S., E. Ziska, V. Brinkmann, U. Zimny-Arndt, A. Fauconnier, P. R. Jungblut, M. Naumann, and T. F. Meyer. 2000. Translocation of the Helicobacter pylori CagA protein in gastric epithelial cells by a type IV secretion apparatus. Cell. Microbiol. 2:155-164.
11. Censini, S., C. Lange, Z. Xiang, J. E. Crabtree, P. Ghiara, M. Borodovsky, R. Rappuoli, and A. Covacci. 1996. cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors. Proc. Natl. Acad. Sci. USA 93:14648-14653.
12. Covacci, A., S. Censini, M. Bugnoli, R. Petracca, D. Burroni, G. Macchia, A. Massone, E. Papini, Z. Xiang, N. Figura, and R. Rappuoli. 1993. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc. Natl. Acad. Sci. USA 90:5791-5795.
13. de Sousa, D., L. J. Fabri, A. Nash, D. J. Hilton, N. A. Nicola, and M. Baca. 2002. SH2 domains from suppressor of cytokine signaling-3 and protein tyrosine phosphatase SHP-2 have similar binding specificities. Biochemistry 41:9229-9236.
14. El-Omar, E. M., K. Oien, L. S. Murray, A. El-Nujumi, A. Wirz., D. Gillen, C. Williams, G. Fullarton, and K. E. L. McColl. 2000. Increased prevalence of precancerous changes in relatives of gastric cancer patients: critical role of Helicobacter pylori. Gastroenterology 118:22-30.
15. Higashi, H., R. Tsutsumi, A. Fujita, S. Yamazaki, M. Asaka, T. Azuma, and M. Hatakeyama. 2002. Biological activity of the Helicobacter pylori virulence factor CagA is determined by variation in the tyrosine phosphorylation sites. Proc. Natl. Acad. Sci. USA 99:14428-14433.
16. Higashi, H., R. Tsutsumi, S. Muto, T. Sugiyama, T. Azuma, M. Asake, and M. Hatakeyama. 2002. SHP-2 tyrosine phosphatase as an intracellular target of Helicobacter pylori CagA protein. Science 295:683-686.
17. Jenks, P. J., F. Mégraud, and A. Labigne. 1998. Clinical outcome after infection with Helicobacter pylori does not appear to be reliably predicted by the presence of any of the genes of the cag pathogenicity island. Gut 43:752-758.
18. Kidd, M., A. J. Lastovica, J. C. Atherton, and J. A. Louw. 2001. Conservation of the cag pathogenicity island is associated with vacA alleles and gastroduodenal disease is South African Helicobacter pylori isolates. Gut 49:11-17.
19. Maeda, S., H. Yoshida, T. Ikenoue, K. Ogura, F. Kanai, N. Kato, Y. Shiratori, and M. Omata. 1999. Structure of cag pathogenicity island in Japanese Helicobacter pylori isolates. Gut 44:336-341.
20. Mimuro, H., T. Suzuki, J. Tanaka, M. Asahi, R. Haas, and C. Sasakawa. 2002. Grb2 is a key mediator of Helicobacter pylori CagA protein activities. Mol. Cell 10:745-755.
21. Nilsson, C., A. Sillén, L. Eriksson, M.-L. Strand, H. Enroth, S. Normark, P. Falk, and L. Engstrand. 2003. Correlation between cag pathogenicity island composition and Helicobacter pylori-associated gastroduodenal disease. Infect. Immun. 71:6573-6581.
22. Odenbreit, S., J. Püls, B. Sedlmaier, E. Gerland, W. Fischer, and R. Haas. 2000. Translocation of Helicobacter pylori CagA into gastric epithelial cells by type IV secretion. Science 287:1497-1500.
23. Owen, R. J., S. I. Sharp, S. A. Chisholm, and S. Rijpkema. 2003. Identification of cagA tyrosine phosphorylation DNA motifs in Helicobacter pylori isolates from peptic ulcer patients by novel PCR-restriction fragment length polymorphism and real-time fluorescence PCR assays. J. Clin. Microbiol. 41:3112-3118.
24. Rudi, J., C. Kolb, M. Maiwald, D. Kuck, A. Sieg, P. R. Galle, and W. Stremmel. 1998. Diversity of Helicobacter pylori vacA and cagA genes and relationship to VacA and CagA protein expression, cytotoxin production, and associated diseases. J. Clin. Microbiol. 36:944-948.
25. Segal, E. D., J. Cha, J. Lo, S. Falkow, and L. S. Tompkins. 1999. Altered states: involvement of phosphorylated CagA in the induction of host cellular growth changes by Helicobacter pylori. Proc. Natl. Acad. Sci. USA 96:14559-14564.
26. Segal, E. D., S. Falkow, and L. S. Tompkins. 1996. Helicobacter pylori attachment to gastric cells induces cytoskeletal rearrangements and tyrosine phosphorylation of host cell proteins. Proc. Natl. Acad. Sci. USA 93:1259-1264.
27. Selbach, M., S. Moese, C. R. Hauck, T. F. Meyer, and S. Backert. 2002. Src is the kinase of the Helicobacter pylori CagA protein in vitro and in vivo. J. Biol. Chem. 277:6775-6778.
28. Stein, M., R. Rappuoli, and A. Covacci. 2000. Tyrosine phosphorylation of the Helicobacter pylori CagA antigen after cag-driven host cell translocation. Proc. Natl. Acad. Sci. USA 97:1263-1268.
29. Stein, M., F. Bagnoli, R. Halenbeck, R. Rappuoli, W. J. Fantl, and A. Covacci. 2002. c-Src/Lyn kinases activate Helicobacter pylori CagA through tyrosine phosphorylation of the EPIYA motifs. Mol. Microbiol. 43:971-980.
30. Tsutsumi, R., H. Higashi, M. Higuchi, M. Okada, and M. Hatakeyama. 2003. Attenuation of Helicobacter pylori CagA·SHP-2 signaling by interaction between CagA and C-terminal Src kinase. J. Biol. Chem. 278:3664-3670.
31. Tummuru, M. K. R., S. A. Sharma, and M. J. Blaser. 1995. Helicobacter pylori picB, a homologue of the Bordetella pertussis toxin secretion protein, is required for induction of IL-8 in gastric epithelial cells. Mol. Microbiol. 18:867-876.
32. Yamaoka, Y., T. Kodama, K. Kashima, D. Y. Graham, and A. R. Sepulveda. 1998. Variants of the 3′ region of the cagA gene in Helicobacter pylori isolates from patients with different H. pylori-associated diseases. J. Clin. Microbiol. 36:2258-2263.
33. Yamazaki, S., A. Yamakawa, Y. Ito, M. Ohtani, H. Higashi, M. Hatakeyama, and T. Azuma. 2003. The CagA protein of Helicobacter pylori is translocated into epithelial cells and binds to SHP-2 in human gastric mucosa. J. Infect. Dis. 187:334-337.