Diversiyt of Helicobacter pylori vacA and cagA genes and relationship to VacA and CagA protein expression, cytotoxin production, and associated disease

Journal of Clinical Microbiology

Volumn 36, Issue 4, 1998, Pages 944-948

  Rudi, Jochen ^a   Kolb, Christof ^a   Maiwald, Matthias ^a   Kuck, Dirk ^a   Sieg, Andreas ^a   Galle, Peter R ^a   Stremmel, Wolfgang ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOTOXIN; BACTERIAL ANTIGEN; BACTERIAL PROTEIN; CAGA PROTEIN, HELICOBACTER PYLORI; VACA PROTEIN, HELICOBACTER PYLORI;

ARTICLE; CHRONIC GASTRITIS; CYTOTOXICITY; GENETIC HETEROGENEITY; GENOTYPE; HUMAN; HUMAN CELL; HUMAN TISSUE; MAJOR CLINICAL STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PEPTIC ULCER; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; STOMACH CANCER; BACTERIAL GENE; BIOSYNTHESIS; GENETICS; HELA CELL; HELICOBACTER PYLORI; PATHOGENICITY;

HELICOBACTER PYLORI;

ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; CYTOTOXINS; GENES, BACTERIAL; GENOTYPE; HELA CELLS; HELICOBACTER PYLORI; HUMANS;

EID: 0031594103     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.36.4.944-948.1998     Document Type: Article

References (30)

1. Atherton, J. C., P. Cao, R. M. Peek, M. K. R. Tummuru, M. J. Blaser, and T. L. Cover. 1995. Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. J. Biol. Chem. 270:17771-17777.
2. Atherton, J. C., R. M. Peek, K. T. Tham, T. L. Cover, and M. J. Blaser. 1997. Clinical and pathological importance of heterogeneity in vacA, the vacuolating cytotoxin gene of Helicobacter pylori. Gastroenterology 112:92-99.
3. Censini, S., C. Lange, Z. Xiang, J. E. Crabtree, P. Ghiara, M. Borodovsky, R. Rappuoli, and A. Covacci. 1996. cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors. Proc. Natl. Acad. Sci. USA 93:14648-14653.
4. Covacci, A., S. Censini, M. Bugnoli, R. Petracca, D. Burroni, G. Macchia, A. Massone, E. Papini, Z. Xiang, N. Figura, and R. I. Rappuoli. 1993 Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc. Natl. Acad. Sci. USA 90:5791-5795.
5. Cover, T. L., P. Cao, C. D. Lind, K. T. Tham, and M. J. Blaser. 1993. Correlation between vacuolating cytotoxin production by Helicobacter pylori isolates in vitro and in vivo. Infect. Immun. 61:5008-5012.
6. Cover, T. L., C. P. Dooley, and M. J. Blaser. 1990. Characterization of and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolizing cytotoxin activity. Infect. Immun. 58:603-610.
7. Cover, T. L., W. Puryear, G. I. Perez-Perez, and M. J. Blaser. 1991. Effect of urease on HeLa cell vacuolation induced by Helicobacter pylori cytotoxin. Infect. Immun. 59:1264-1270.
8. Cover, T. L., M. K. R. Tummuru, P. Cao, S. A. Thompson, and M. J. Blaser. 1994. Divergence of genetic sequences for the vacuolating cytotoxin among Helicobacter pylori strains. J. Biol. Chem. 269:10566-10573.
9. Cover, T. L., S. G. Vaughn, P. Cao, and M. J. Blaser. 1992. Potentiation of Helicobacter pylori vacuolating cytotoxin activity by nicotine and other weak bases. J. Infect. Dis. 166:1073-1078.
10. Crabtree, J. E., N. Figura, J. D. Taylor, M. Bugnoli, D. Armellini, and D. S. Tompkins. 1992. Expression of 120 kilodalton protein and cytotoxicity in Helicobacter pylori. J. Clin. Pathol. 45:733-734.
11. Crabtree, J. E., J. D. Taylor, J. I. Wyatt, R. V. Heatley, T. M. Shallcross, D. S. Tompkins, and B. J. Rathbone. 1991. Mucosal IgA recognition of Helicobacter pylori 120 kDa protein, peptic ulceration and gastric pathology. Lancet 59:1264-1270.
12. Figura, N., P. Guglielmetti, A. Rossolini, A. Barberi, G. Cusi, R. A. Musmanno, M. Russi, and S. Quaranta. 1989. Cytotoxin production by Campylobacter pylori strains isolated from patients with peptic ulcers and from patients with chronic gastritis only. J. Clin. Microbiol. 27:225-226.
13. Goosens, H., Y. Glupczynski, A. Burette, J.-P. Lambert, L. Vlaes, and J.-P. Butzler. 1992. Role of the vacuolating toxin from Helicobacter pylori in the pathogenesis of duodenal and gastric ulcer. Med. Microbiol. Lett. 1:153-159.
14. Harris, R. P., T. L. Cover, D. R. Crowe, J. M. Orenstein, M. F. Graham, M. J. Blaser, and P. D. Smith. 1996. Helicobacter pylori cytotoxin induces vacuolation of primary human mucosal epithelial cells. Infect. Immun. 64:4867-4871.
15. Hentschel, I., G. Brandstätter, B. Dragosics, A. M. Hirschl, H. Nemec, K. Schütze, M. Taufer, and H. Wurzer. 1993. Effect of ranitidine and amoxicillin plus metronidazole on the eradication of Helicobacter pylori in the pathogenensis of duodenal and gastric ulcer. N. Engl. J. Med. 328:308-312.
16. Leunk, R. D., P. T. Johnson, B. C. David, W. G. Kraft, and D. R. Morgan. 1988. Cytotoxic activity in broth-culture filtrates of Campylobacter pylori. J. Med. Microbiol. 26:93-99.
17. Maiwald, M., H.-J. Ditton, H.-G. Sonntag, and M. von Knebel Doeberitz. 1994. Characterization of contaminating DNA in Taq polymerase which occurs during amplification with a primer set for Legionella 5S ribosomal RNA. Mol. Cell. Probes 8:11-14.
18. Parsonnet, J., G. D. Friedman, D. P. Vandersteen, Y. Chang, J. H. Vogelman, N. Orenteich, and R. K. Sibley. 1991. Helicobacter pylori infection and the risk of gastric carcinoma. N. Engl. J. Med. 325:1127-1231.
19. Rudi, J. Unpublished observation.
20. Rudi, J., C. Kolb, M. Maiwald, I. Zuna, P. R. Galle, and W. Stremmel. 1997. Serum antibodies against the Helicobacter pylori proteins CagA and VacA are associated with an increased risk for gastric adenocarcinoma. Dig. Dis. Sci. 42:1652-1659.
21. Schmitt, W., and R. Haas. 1994. Genetic analysis of the Helicobacter pylori vacuolating cytotoxin: structural similarities with the IgA protease type of exported protein. Mol. Microbiol. 12:307-319.
22. Taylor, D. N., and M. J. Blaser. 1991. The epidemiology of Helicobacter pylori infection. Epidemiol. Rev. 13:42-59.
23. Tummuru, M. K. R., T. L. Cover, and M. J. Blaser. 1993 Cloning and expression of a high-molecular-mass major antigen of Helicobacter pylori: evidence of linkage to cytotoxin production. Infect. Immun. 61:1799-1809.
24. Tummuru, M. K. R., T. L. Cover, and M. J. Blaser. 1994. Mutation of the cytotoxin-associated cagA gene does not affect the vacuolating cytotoxin activity of Helicobacter pylori. Infect. Immun. 62:2609-2613.
25. Tummuru, M. K., S. A. Sharma, and M. J. Blaser. 1995. Helicobacter pylori picB, a homologue of the Bordetella pertussis toxin secretion protein, is required for induction of IL-8 in gastric epithelial cells. Mol. Microbiol. 18: 867-876.
26. Warren, J. R., and B. J. Marshall. 1983. Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet i:1273-1274.
27. Weel, J. F. L., R. W. M. van der Hulst, Y. Gerrits, P. Roorde, M. Feller, J. Dankert, G. N. J. Tytgat, and A. van der Ende. 1996. The interrelationship between cytotoxin-associated gene A, vacuolating cytotoxin, and Helicobacter pylori-related diseases. J. Infect. Dis. 173:1171-1175.
28. Wotherspoon, A. C., C. Ortiz-Hidalgo, M. R. Falzon, and P. G. Isaacson. 1991. Helicobacter pylori-associated gastritis and primary B-cell gastric lymphoma. Lancet 338:1175-1176.
29. Xiang, Z., M. Bugnoli, A. Ponzetto, A. Morgando, N. Figura, A. Covacci, R. Petracca, C. Pennatini, S. Censini, D. Armellini, and R. Rappuoli. 1993. Detection in an enzyme immunoassay of an immune response to a recombinant fragment of the 128-kilodalton protein (CagA) of Helicobacter pylori. Eur. J. Clin. Microbiol. Infect. Dis. 12:739-745.
30. Xiang, Z., S. Censini, P. F. Bayelli, J. L. Telford, N. Figura, R. Rappuoli, and A. Covacci. 1995. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin. Infect. Immun. 63:94-98.