Production of biologically active tethered ovine FSHβα by the methylotrophic yeast Pichia pastoris

Journal of Molecular Endocrinology

Volumn 30, Issue 2, 2003, Pages 213-225

  Fidler, A E ^a   Lin, J S ^a   Lun, S ^a   Ng Chie, W ^a   Western, A ^a   Stent, V ^a   McNatty, Kenneth P ^a  

^a AGRESEARCH   (New Zealand)

Author keywords

[No Author keywords available]

Indexed keywords

ACID PHOSPHATASE; FOLLITROPIN; FOLLITROPIN RECEPTOR; GLYCOPROTEIN; HYBRID PROTEIN; POLYPEPTIDE; PROTEIN SUBUNIT; RECOMBINANT PROTEIN;

ALPHA CHAIN; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BETA CHAIN; BIOASSAY; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; FUSION GENE; GENETIC CODE; HORMONE SYNTHESIS; IN VITRO STUDY; MOLECULAR WEIGHT; NONHUMAN; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECEPTOR BINDING; YEAST;

ANIMALS; ARTIFICIAL GENE FUSION; CATTLE; CODON; FEMALE; FOLLICLE STIMULATING HORMONE, BETA SUBUNIT; GENETIC ENGINEERING; GLYCOPROTEIN HORMONES, ALPHA SUBUNIT; PICHIA; RECEPTORS, FSH; RECOMBINANT FUSION PROTEINS; SHEEP;

EID: 0038702056     PISSN: 09525041     EISSN: None     Source Type: Journal    
DOI: 10.1677/jme.0.0300213     Document Type: Article

References (33)

1. Albanese C, Christin-Maitre S, Sluss PM, Crowley WF & Jameson JL, 1994 Development of a bioassay for FSH using a recombinant human FSH receptor and a cAMP responsive luciferase reporter gene. Molecular and Cellular Endocrinology 101 211-219.
2. Anderiesz C, Ferraretti A, Magli C, Fiorentino A, Fortini D, Gianaroli L, Jones GM & Trounson AO 2000 Effect of recombinant human gonadotrophins on human, bovine and murine oocyte meiosis, fertilization and embryonic development in vitro. Human Reproduction 15 1140-1148.
3. Barr KA, Hopkins SA & Sreekrishna K 1992 Protocol for efficient secretion of HSA developed from Pichia pastoris. Pharmaceutical Engineering 12 48-51.
4. Batard Y, Hehn A, Nedelkina S, Schalk M, Pallett K, Schaller H & Werck-Reichart D 2000 Increasing expression of P450 and P450-reductase proteins from monocots in heterologous systems. Archives of Biochemistry and Biophysics 379 161-169.
5. Bello PA, Mountford PS, Brandon MR & Adams TE 1989 Cloning and DNA sequence analysis of the cDNA for the common α-subunit of the ovine pituitary glycoprotein hormones. Nucleic Acids Research 17 10494.
6. Ben-Menahen D, Hyde R, Pixely M, Beger P & Biome I 1999 Synthesis of multi-subunit domain gonadotrophin complexes: a model for alpha/beta heterodimer formation. Biochemistry 38 15070-15077.
7. Combarnous Y 1992 Molecular basis of the specificity of binding of glycoprotein hormones to their receptors. Endocrine Reviews 13 670-691.
8. Devroey P, Mannaerts B, Smitz J, Coelingh Bennink H & Van Steirteghem A 1993 First established pregnancy and birth after ovarian stimulation with recombinant human follicle stimulating hormone (Org 32489). Human Reproduction 8 863-865.
9. Dohmen RJ, Strasser AWM, Honer CB & Hollenberg CP 1991 An efficient transformation procedure enabling long-term storage of competent cells of various yeast genera. Yeast 7 691-692.
10. Esch FS, Mason AJ, Cooksey K, Mercado M & Shimasaki S 1986 Cloning and DNA sequence analysis of the cDNA for the precursor of the beta chain of bovine follicle stimulating hormone. PNAS 83 6618-6621.
11. Fidler AE, Lun S, Young W & McNatty KP 1998 Expression and secretion of a biologically active glycoprotein hormone, ovine follicle stimulating hormone, by Pichia pastoris. Journal of Molecular Endocrinology 21 327-336.
12. Gleeson MA, White CE, Meininger DP & Komives EA 1998 Generation of protease-deficient strains and their use in heterologous protein expression. Methods in Molecular Biology 103 81-94.
13. Grinna LS & Tschopp JF 1989 Size distribution and general structural features of N-linked oligosaccharides from the methylotrophic yeast, Pichia pastoris. Yeast 5 107-113.
14. Hirai T, Takikawa H & Kato Y 1989 Molecular cloning of cDNAs for precursors of porcine pituitary glycoprotein hormone common α-subunit and of thyroid stimulating hormone β-subunit. Molecular and Cellular Endocrinology 63 208-217.
15. Hollenberg CP & Gellisen G 1997 Production of recombinant proteins by methylotrophic yeasts. Current Opinion in Biotechnology 8 554-560.
16. Horton RM, Cai Z, Ho SN & Pease LR 1990 Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction. Biotechniques 8 528-535.
17. Kato Y 1988 Cloning and DNA sequence analysis of the cDNA for the precursor of porcine follicle stimulating hormone (FSH) beta subunit. Molecular and Cellular Endocrinology 55 107-112.
18. de Koning WJ, Walsh GA, Wrynn S & Headon DR 1994 Recombinant reproduction. Bio / Technology 12 988-992.
19. Kumar TR, Wang Y, Lu N & Matzuk MM 1997 Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility. Nature Genetics 15 201-204.
20. McNatty KP, Heath DA, Lun S & Hudson NL 1989 Adenosine 3′,5-cyclic monophosphate phosphodiesterase activity in granulosa cells from Booroola x Romney ewes with and without the F gene. Journal of Endocrinology 120 287-293.
21. Mountford PS, Bello PA, Brandon MR & Adams TE 1989 Cloning and DNA sequence analysis of the cDNA for the precursor of ovine follicle stimulating hormone β-subunit. Nucleic Acids Research 17 6391.
22. Moore LG, Ng-Chie W, Lun S, Lawrence SB, Young W & McNatty KP 1997 Follicle-stimulating hormone in the brushtail possum (Trichosurus vulpecula): purification, characterization, and radoimmunoassay. General and Comparative Endocrinology 106 30-38.
23. Moore LG, Ng-Chie W, Hudson NL, & McNatty KP 2000 Isoforms and half-life of FSH from sheep with different reproductive states. Journal of Endocrinology 165 185-192.
24. Nagaya T & Jameson JL 1994 Structural features of the glycoprotein hormones genes and their encoded proteins. In The Pituitary Gland, pp 63 89. Ed. H Imura. New York: Raven Press.
25. Reichert LE & Wilhelmi AE 1978 Biological activities of recent preparations of pituitary hormones produced under the programs of the National Institute of Arthritis, Metabolism, and Digestive Diseases. Endocrinology 102 982-983.
26. Richard F, Robert P, Remy JJ, Martinat N, Bidart JM, Salesse R & Combarnous Y 1998 High-level secretion of biologically active recombinant porcine follicle-stimulating hormone by the methylotrophic yeast Pichia pastoris. Biochemical and Biophysical Research Communications 245 847-852.
27. Richards JS 1994 Hormonal control of gene expression in the ovary. Endocrine Reviews 15 725-751.
28. Samaddar M, Catterall JF & Dighe RR 1997 Expression of biologically active beta subunit of bovine follicle-stimulating hormone in the methylotrophic yeast Pichia pastoris. Protein Expression and Purification 10 343-355.
29. Sen Gupta C & Dighe RR 1999 Hyperexpression of biologically active human chorionic gonadotrophin using the methylotropic yeast, Pichia pastoris. Journal of Molecular Endocrinology 22 273-283.
30. Sen Gupta C & Dighe RR 2000 Biological activity of single chain chorionic gonadotropin, hCGalphabeta, is decreased upon deletion of five carboxyl terminal amino acids of the alpha subunit without affecting its receptor binding. Journal of Molecular Endocrinology 24 157-164.
31. Sreekrishna K & Kropp KE 1996 Pichia pastoris. In Nonconventional Yeasts in Biotechnology. A handbook, pp 203-253. Ed. K Wolf. Berlin: Springer-Verlag.
32. Sudbery P 1996 The expression of recombinant proteins in yeasts. Current Opinion in Biotechnology 7 517-524.
33. Ulloa-Aguirre A, Midgley JR. AR, Beitins IZ & Padmanabhan V 1995 Follicle-stimulating isohormones: characterization and physiological relevance. Endocrine Reviews 16 765-787.