Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase δ subunit Cdc27

BMC Molecular Biology

Volumn 5, Issue , 2004, Pages

  Gray, Fiona C ^a   Pohler, J Richard G ^b   Warbrick, Emma ^b   MacNeill, Stuart A ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA DIRECTED DNA POLYMERASE DELTA; DNA POLYMERASE; CDC27 PROTEIN, HUMAN; CDC27 PROTEIN, S POMBE; CELL CYCLE PROTEIN; DNA DIRECTED DNA POLYMERASE; DNA DIRECTED DNA POLYMERASE BETA; DNA DIRECTED DNA POLYMERASE GAMMA; FUNGAL DNA; RECOMBINANT PROTEIN; SCHIZOSACCHAROMYCES POMBE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; DNA REPLICATION; ENZYME SUBUNIT; FUNGAL STRAIN; GENE MAPPING; GENE MUTATION; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PROTEIN INTERACTION; PROTEIN MOTIF; YEAST; BIOSYNTHESIS; CHEMISTRY; ENZYME ACTIVE SITE; ENZYMOLOGY; GENE EXPRESSION; GENETICS; HUMAN; METABOLISM; MUTATION; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE; SCHIZOSACCHAROMYCES; TWO HYBRID SYSTEM;

EUKARYOTA; MAMMALIA; SCHIZOSACCHAROMYCES POMBE;

AMINO ACID MOTIFS; CATALYTIC DOMAIN; CELL CYCLE PROTEINS; CONSERVED SEQUENCE; DNA POLYMERASE I; DNA POLYMERASE III; DNA REPLICATION; DNA, FUNGAL; DNA-DIRECTED DNA POLYMERASE; GENE EXPRESSION; HUMANS; MUTATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 13444291950     PISSN: 14712199     EISSN: 14712199     Source Type: Journal    
DOI: 10.1186/1471-2199-5-21     Document Type: Article

References (43)

1. Waga S, Stillman B: The DNA replication fork in eukaryotic cells. Ann Rev Biochem 1998, 67:721-751.
2. MacNeill SA, Burgers PMJ: Chromosomal DNA replication in yeast: enzymes and mechanisms. In The Yeast Nucleus Edited by: Fantes P and Beggs J. Oxford, Oxford University Press; 2000:19-57.
3. Pavlov YI, Maki S, Maki H, Kunkel TA: Evidence for interplay among yeast replicative DNA polymerases α, δ and ε from studies of exonuclease and polymerase active site mutations. BMC Biol 2004, 2:11.
4. Kesti T, Flick K, Keranen S, Syvaoja JE, Wittenberg C: DNA polymerase ε catalytic domains are dispensable for DNA replication, DNA repair, and cell viability. Molecular Cell 1999, 3:679-685.
5. Dua R, Levy DL, Campbell JL: Analysis of the essential functions of the C-terminal protein/protein interaction domain of Saccharomyces cerevisiae pol ε and its unexpected ability to support growth in the absence of the DNA polymerase domain. J Biol Chem 1999, 274:22283-22288.
6. Ohya T, Kawasaki Y, Hiraga S, Kanbara S, Nakajo K, Nakashima N, Suzuki A, Sugino A: The DNA polymerase domain of pol ε is required for rapid, efficient, and highly accurate chromosomal DNA replication, telomere length maintenance, and normal cell senescence in Saccharomyces cerevisiae. J Biol Chem 2002, 277:28099-28108.
7. Feng W, D'Urso G: Schizosaccharomyces pombe cells lacking the amino-terminal catalytic domains of DNA polymerase ε are viable but require the DNA damage checkpoint control. Mol Cell Biol 2001, 21:4495-4504.
8. Wang TSF: Cellular DNA polymerases. In DNA Replication in Eukaryotic Cells Edited by: DePamphilis ML. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory Press; 1996:461-493.
9. Aravind L, Koonin EV: Phosphoesterase domains associated with DNA polymerases of diverse origins. Nucl Acids Res 1998, 26:3746-3752.
10. Makiniemi M, Pospjech H, Kilpelainen S, Jokela M, Vihinen M, Syvaoja JE: A novel family of DNA-polymerase-associated B subunits. Trends in biochemical sciences 1999, 24:14-16.
11. Cip1-like PCNA binding motif. EMBO J 2000, 19:1108-1118.
12. Bermudez VP, MacNeill SA, Tappin I, Hurwitz J: The influence of the Cdc27 subunit on the properties of the Schizosaccharomyces pombe DNA polymerase δ. J Biol Chem 2002, 277:36853-36862.
13. Reynolds N, Watt A, Fantes PA, MacNeill SA: Cdm1, the smallest subunit of DNA polymerase δ in the fission yeast Schizosaccharomyces pombe, is non-essential for growth and division. Curr Genet 1998, 34:250-258.
14. Podust VN, Chang LS, Ott R, Dianov GL, Fanning E: Reconstitution of human DNA polymerase δ using recombinant baculoviruses: the p12 subunit potentiates DNA polymerizing activity of the four-subunit enzyme. J Biol Chem 2002, 277:3894-3901.
15. Fukui T, Yamauchi K, Muroya T, Akiyama M, Maki H, Sugino A, Waga S: Distinct roles of DNA polymerases δ and ε at the replication fork in Xenopus egg extracts. Genes Cells 2004, 9:179-191.
16. Huang ME, LeDouarin B, Henry C, Galibert F: The Saccharomyces cerevisiae protein YJR043C (Pol32) interacts with the catalytic subunit of DNA, polymerase α and is required for cell cycle progression in G2/M. Mol Gen Genet 1999, 260:541-550.
17. Johansson E, Garg P, Burgers PM: The Pol32 subunit of DNA polymerase δ contains separable domains for processive replication and proliferating cell nuclear antigen (PCNA) binding. J Biol Chem 2004, 279:1907-1915.
18. MacNeill SA, Moreno S, Reynolds N, Nurse P, Fantes PA: The fission yeast Cdc1 protein, a homolog of the small subunit of DNA polymerase δ, binds to Pol3 and Cdc27. EMBO Journal 1996, 15:4613-4628.
19. Gerik KJ, Li XY, Pautz A, Burgers PMJ: Characterization of the two small subunits of Saccharomyces cerevisiae DNA polymerase δ. J Biol Chem 1998, 273:19747-19755.
20. Burgers PMJ, Gerik KJ: Structure and processivity of two forms of Saccharomyces cerevisiae DNA polymerase δ. J Biol Chem 1998, 273:19756-19762.
21. Hughes P, Tratner I, Ducoux M, Piard K, Baldacci G: Isolation and identification of the third subunit of mammalian DNA polymerase δ by PCNA-affinity chromatography of mouse FM3A cell extracts. Nucleic Acids Res 1999, 27:2108-2114.
22. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ: Basic local alignment search tool. J Mol Biol 1990, 215:403-410.
23. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997, 25:3389-3402.
24. Altschul SF, Koonin EV: Iterated profile searches with PSIBLAST - a tool for discovery in protein databases. Trends Biochem Sci 1998, 23:444-447.
25. Bhaumik D, Wang TSF: Mutational effect of fission yeast Pol α on cell cycle events. Mol Biol Cell 1998, 9:2107-2123.
26. D'Urso G, Grallert B, Nurse P: DNA polymerase a, a component of the replication initiation complex, is essential for the checkpoint coupling S-phase to mitosis in fission yeast. J Cell Sci 1995, 108:3109-3118.
27. Murakami H, Okayama H: A kinase from fission yeast responsible for blocking mitosis in S phase. Nature 1995, 374:817-819.
28. Maundrell K: nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine. J Biol Chem 1990, 265:10857-10864.
29. Maundrell K: Thiamine-repressible expression vectors pREP and pRIP for fission yeast. Gene 1993, 123:127-130.
30. Francesconi S, Park H, Wang TSF: Fission yeast with DNA polymerase δ temperature-sensitive alleles exhibits cell division cycle phenotype. Nucleic Acids Res 1993, 21:3821-3828.
31. Kang HY, Choi E, Bae SH, Lee KH, Gim BS, Kim HD, Park C, MacNeill SA, Seo YS: Genetic analyses of Schizosaccharomyces pombe dna2+ reveal that dna2 plays an essential role in Okazaki fragment metabolism. Genetics 2000, 155:1055-1067.
32. Gould KL, Burns CG, Feoktistova A, Hu CP, Pasion SG, Forsburg SL: Fission yeast cdc24+ encodes a novel replication factor required for chromosome integrity. Genetics 1998, 149:1221-1233.
33. Tanaka H, Tanaka K, Murakami H, Okayama H: Fission yeast Cdc24 is a replication factor C- and proliferating cell nuclear antigen-interacting factor essential for S-phase completion. Mol Cell Biol 1999, 19:1038-1048.
34. Bentley NJ, Holtzman DA, Flaggs G, Keegan KS, DeMaggio A, Ford JC, Hoekstra M, Carr AM: The Schizosaccharomyces pombe rad3 checkpoint gene. EMBO J 1996, 15:6641-6651.
35. Carr AM: DNA structure dependent checkpoints as regulators of DNA repair. DNA Repair (Amst) 2002, 1:983-994.
36. Zuo S, Gibbs E, Kelman Z, Wang TS, O'Donnell M, MacNeill SA, Hurwitz J: DNA polymerase δ isolated from Schizosaccharomyces pombe contains five subunits. Proc Natl Acad Sci U S A 1997, 94:11244-11249.
37. Johansson E, Majka J, Burgers PM: Structure of DNA polymerase δ from Saccharomyces cerevisiae. J Biol Chem 2001, 276:43824-43828.
38. Moreno S, Klar A, Nurse P: Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods in enzymology 1991, 194:795-823.
39. Prentice HL: High-efficiency transformation of Schizosaccharomyces pombe by electroporation. Nucleic acids research 1992, 20:621.
40. Bähler J, Wu JQ, Longtine MS, Shah NG, McKenzie A, Steever AB, Wach A, Philippsen P, Pringle JR: Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast 1998, 14:943-951.
41. Miller JH: Experiments in Molecular Genetics. Cold Spring Harbor, NY., Cold Spring Harbor Laboratory Press; 1972.
42. Nurse P, Thuriaux P, Nasmyth K: Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet 1976, 146:167-178.
43. Nasmyth K, Nurse P: Cell division cycle mutants altered in DNA replication and mitosis in the fission yeast Schizosaccharomyces pombe. Mol Gen Genet 1981, 182:119-124.