Time resolved multiphoton imaging of the interaction between the PKC and NFκB signalling pathways

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 5139, Issue , 2003, Pages 216-222

  Morton, Penny E ^a,b   Ng, Tony C ^b   Roberts, Sarah A ^a   Vojnovic, Borivoj ^a   Ameer Beg, Simon M ^a  

^a MOUNT VERNON HOSPITAL   (United Kingdom)

^b KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

FLIM; FRET; Multiphoton; NF B; PKC

Indexed keywords

CELLS; FLUORESCENCE; OPTICAL MICROSCOPY; PROTEINS;

FLUORESCENCE LIFETIME IMAGING MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; MULTIPHOTON IMAGING;

MEDICAL IMAGING;

EID: 1342331882     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.500513     Document Type: Conference Paper

References (22)

1. Ng T, Squire A, Hansra G, Bornancin F, Prevostel C, Hanby A, Harris W, Barnes D, Schmidt S, Mellor H, Bastiaens PIH and Parker PJ (1999). Imaging PKC alpha activation in cells. Science, 283, pp2085-2089.
2. Barnes P.J., Karin M. Nuclear factor-κB - a pivotal transcription factor in chronic inflammatory diseases. N. Engl. J. Med. 334 pp1066-71, 1997.
3. Karin M. How NF-κB is activated: the role of the IκB kinase (IKK) complex. Oncogene 18 pp6867-6874, 1999.
4. Siebenlist U., Franzoso, G, Brown K. Structure, regulation and function of NF-κ-B. Annu. Rev. Cell. Biol. 10 pp504-55, 1994.
5. Ghosh S., May M.J., Kopp E.B. NF-κB and Rel proteins: evolutionary conserved mediators of immune responses. Annual. Rev. Immunol. 14 pp649-81, 1998
6. Karin M and Ben-Neriah Y. Phosphorylation meets ubiquitination: The control of the NF-κB pathway. Ann. Rev. Imm. 18 pp621-663, 2000
7. Brown, K., Gerstberger S., Carlson L., Franzoso G., Siebenlist U. Control of IκBα proteolysis by site-specific, signal-induced phosphorylation. Science 267 pp1485-91, 1993.
8. Didonato J. A., Mercurio F., Rosette C., Wu-Li J., Suyong, H., Ghosh S., Karin M. Mapping of the inducible IκB phosphorylation sites that signal its ubiquitination and degradation. Mol. Cell. Biol. 16 pp295-304, 1996.
9. Whiteside ST, Israël A. IκB proteins: Structure, function and regulation. Semin. Cancer. Biol. 8 pp75-82, 1997
10. Mercurio F., Zhu H., Murray, B. W., Shevchenko A., Bennett B.L., Li, J., Young D.B., Barbosa M., Mann M., Manning, A., Rao, A. IKK-1 and IKK-2: cytokine-activated IκB kinases essential for NF-κB activation. Science 278 pp860-66, 1997.
11. Zandi E., Rothwarf D.M., Delhase, M., Haykawa M., Karin M. The IκB kinase complex (IKK) contains two kinase subunits, IKKα and IKKβ, necessary for IκB phosphorylation and NF-κB activation. Cell 91 pp243-52, 1997.
12. Didonata J.A. Hayakawa M., Rothwarf D.M., Zandi E., Karin M. A cytokine-responsive IκB kinase that activates the transcription factor NF-κB. Nature 288 pp548-554, 1997.
13. Sanz L., Sanchez P., Lallena M-J., Diaz-Meco M. T. and Moscat J. The interaction of p62 with RIP links the atypical PKCs to NF-κB activation. EMBO J. 18 pp3044-3053, 1999.
14. Baud V., Liu Z-G., Bennet B., Suzuxi N., Xia Y., Karin M. Signaling by proinflammatory cytokines: oligomerization of TRAF2 and TRAF6 is sufficient for JNK and IKK activation and target gene induction via an N-terminal effector domain. Genes Dev. 13 pp1297-306, 1999.
15. Vertegaal ACO, Kuiperij HB, Yamaoka S, Coutois G, Eb V-D AJ, Zantema A. Protein Kinase C-α is an upstream activator of the IκB kinase complex in the TPA signal transduction pathway to NF-κB in U20S cells. Cell. Sig. 12 pp759-768, 2000.
16. Lallena M.J., Diaz-Meco M.T., Bren G., Payá C.V., Moscat J. Activation of IκB kinase beta by protein kinase C isoforms. Mol. Cell. Biol. 19 pp2180-88.
17. Saijo K, Mecklenbräucker I, Santana A, Leitger M, Schmedt C, Tarakhovsky A. Protein Kianse C β controls nuclear factor κ B activation in B cells through selective regulation of the IκK kinase α. J. Exp. Med. 195, pp1647-1652, 2002.
18. Schmid JA, Birbach A, Hofer-Warbinek R, Pengg M, Burner U, Furtmüller PG, Binder BR, Martin de R. Dynamics of NF-κB and IκBα studied with green fluorescent protein (GFP) fusion protein. J. Biol. Chem. 275 pp17035-17042, 2000.
19. Förster Th. Zwischenmolekulare Energiewanderung und fluoreszenz. Annalen der Physik 6 pp55-75, 1948.
20. Tramier, M., Gautier, I., Piolot, T., Ravalet, S., Kemnitz, K., Coppey, J., Durieux, C., Mignotte, V. and Coppey-Moisan, M. Picosecond-Hetero-FRET Microscopy to Probe Protein-Protein Interactions in Live Cells. Biophys J, 83, pp3570-7, 2002.
21. Campbell, R.E., Tour, O., Palmer, A.E., Steinbach, P.A., Baird, G.S., Zacharias, D.A. and Tsien, R.Y. A monomeric red fluorescent protein. Proc Natl Acad Sci U S A, 99 p7877-82, 2002.
22. Tsien RY, The green fluorescent protein. Annu. Rev. Biochem. 67 pp509-44