Visualization of looping involving the immunoglobulin heavy-chain locus in developing B cells

Genes and Development

Volumn 19, Issue 3, 2005, Pages 322-327

  Sayegh, Camil ^a,c   Jhunjhunwala, Suchit ^a   Riblet, Roy ^b   Murre, Cornelis ^a  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^b TORREY PINES INSTITUTE FOR MOLECULAR STUDIES   (United States)

^c MERCK FROSST CENTRE FOR THERAPEUTIC RESEARCH   (Canada)

Author keywords

E2A deficient hematopoietic progenitors; Immunoglobulin heavy chain; Locus contraction; Long range interactions; Lymphocytes; V(D)J rearrangement

Indexed keywords

IMMUNOGLOBULIN; IMMUNOGLOBULIN HEAVY CHAIN; RAG1 PROTEIN; RAG2 PROTEIN;

ALLELE; ANALYSIS OF VARIANCE; ANIMAL CELL; ARTICLE; B LYMPHOCYTE; FLUORESCENCE IN SITU HYBRIDIZATION; GENE EXPRESSION; GENE FREQUENCY; GENE INTERACTION; GENE LOCUS; GENE REARRANGEMENT; GENETIC RECOMBINATION; GENETIC VARIABILITY; HEMATOPOIETIC STEM CELL; LYMPHOID CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; T LYMPHOCYTE; THREE DIMENSIONAL IMAGING;

ANIMALS; B-LYMPHOCYTES; CELL DIFFERENTIATION; GENES, IMMUNOGLOBULIN; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN VARIABLE REGION; MICE;

ANIMALIA;

EID: 13244275244     PISSN: 08909369     EISSN: None     Source Type: Journal    
DOI: 10.1101/gad.1254305     Document Type: Article

References (28)

1. Baxter, J., Merkenschlager, M., and Fisher, A.G. 2002. Nuclear organisation and gene expression. Curr. Opin. Cell Biol. 14: 372-376.
2. Bergman, Y. and Cedar, H. 2004. A stepwise epigenetic process controls immunoglobulin allelic exclusion. Nat. Rev. Immunol. 4: 753-761.
3. Carter, D., Chakalova, L., Osborne, C.S., Dai, Y.F., and Fraser, P. 2002. Long-range chromatin regulatory interactions in vivo. Nat. Genet. 32: 623-626.
4. Chevillard, C., Ozaki, J., Herring, C.D., and Riblet, R. 2002. A three-megabase yeast artificial chromosome contig spanning the C57BL mouse Igh locus. J. Immunol. 168: 5659-5666.
5. Chowdhury, D. and Sen, R. 2001. Stepwise activation of the immunoglobulin μ heavy chain gene locus. EMBO J. 20: 6394-6403.
6. Eastman, Q.M., Leu, T.M., and Schatz, D.G. 1996. Initiation of V(D)J recombination in vitro obeying the 12/23 rule. Nature 380: 85-88.
7. Felsenfeld, G. and Groudine, M. 2003. Controlling the double helix. Nature 421: 448-453.
8. Fuxa, M., Skok, J., Souabni, A., Salvagiotto, G., Roldan, E., and Busslinger, M. 2004. Pax5 induces V-to-DJ rearrangements and locus contraction of the immunoglobulin heavy-chain gene. Genes & Dev. 18: 411-422.
9. Goebel, P., Janney, N., Valenzuela, J.R., Romanow, W.J., Murre, C., and Feeney, A.J. 2001. Localized gene-specific induction of accessibility to V(D)J recombination induced by E2A and early B cell factor in nonlymphoid cells. J. Exp. Med. 194: 645-656.
10. Goebel, P., Montalbano, A., Ayers, N., Kompfner, E., Dickinson, L., Webb, C.F., and Feeney, A.J. 2002. High frequency of matrix attachment regions and cut-like protein x/CCAAT-displacement protein and B cell regulator of IgH transcription binding sites flanking Ig V region genes. J. Immunol. 169: 2477-2487.
11. Hesslein, D.G., Pflugh, D.L., Chowdhury, D., Bothwell, A.L., Sen, R., and Schatz, D.G. 2003. Pax5 is required for recombination of transcribed, acetylated, 5′ IgH V gene segments. Genes & Dev. 17: 37-42.
12. Ikawa, T., Kawamoto, H., Wright, L.Y., and Murre, C. 2004. Long-term cultured E2A-deficient hematopoietic progenitor cells are pluripotent. Immunity 20: 349-360.
13. Jones, J.M. and Gellert, M. 2004. The taming of a transposon: V(D)J recombination and the immune system. Immunol. Rev. 200: 233-248.
14. Jung, D. and Alt, F.W. 2004. Unraveling V(D)J recombination; insights into gene regulation. Cell 116: 299-311.
15. Kosak, S.T., Skok, J.A., Medina, K.L., Riblet, R., Le Beau, M.M., Fisher, A.G., and Singh, H. 2002. Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development. Science 296: 158-162.
16. Mainville, C.A., Sheehan, K.M., Klaman, L.D., Giorgetti, C.A., Press, J.L., and Brodeur, P.H. 1996. Deletional mapping of fifteen mouse VH gene families reveals a common organization for three Igh haplotypes. J. Immunol. 156: 1038-1046.
17. Mombaerts, P., Iacomini, J., Johnson, R.S., Herrup, K., Tonegawa, S., and Papaioannou, V.E. 1992. RAG-1-deficient mice have no mature B and T lymphocytes. Cell 68: 869-877.
18. Ostermeier, G.G., Liu, Z., Martins, R.P., Bharadwaj, R.R., Ellis, J., Draghici, S., and Krawetz, S.A. 2003. Nuclear matrix association of the human β-globin locus utilizing a novel approach to quantitative real-time PCR. Nucleic Acids Res. 31: 3257-3266.
19. Romanow, W.J., Langerak, A.W., Goebel, P., Wolvers-Tettero, I.L., van Dongen, J.J., Feeney, A.J., and Murre, C. 2000. E2A and EBF act in synergy with the V(D)J recombinase to generate a diverse immunoglobulin repertoire in nonlymphoid cells. Mol. Cell 5: 343-353.
20. Schebesta, M., Heavey, B., and Busslinger, M. 2002. Transcriptional control of B-cell development. Curr. Opin. Immunol. 14: 216-223.
21. Seet, C.S., Brumbaugh, R.L., and Kee, B.L. 2004. Early B cell factor promotes B lymphopoiesis with reduced Interleukin 7 responsiveness in the absence of E2A. J. Exp. Med. 199: 1689-1700.
22. Shinkai, Y., Rathbun, G., Lam, K.P., Oltz, E.M., Stewart, V., Mendelsohn, M., Charron, J., Datta, M., Young, F., Stall, A.M., et al. 1992. RAG-2-deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement. Cell 68: 855-867.
23. Spicuglia, S., Kumar, S., Yeh, J.H., Vachez, E., Chasson, L., Gorbatch, S., Cautres, J., and Ferrier, P. 2002. Promoter activation by enhancerdependent and -independent loading of activator and coactivator complexes. Mol. Cell 10: 1479-1487.
24. Steen, S.B., Gomelsky, L., and Roth, D.B. 1996. The 12/23 rule is enforced at the cleavage step of V(D)J recombination in vivo. Genes Cells 1: 543-553.
25. Tolhuis, B., Palstra, R.J., Splinter, E., Grosveld, F., and de Laat, W. 2002. Looping and interaction between hypersensitive sites in the active β-globin locus. Mol. Cell 10: 1453-1465.
26. Urbanek, P., Wang, Z.Q., Fetka, I., Wagner, E.F., and Busslinger, M. 1994. Complete block of early B cell differentiation and altered patterning of the posterior midbrain in mice lacking Pax5/BSAP. Cell 79: 901-912.
27. Yancopoulos, G.D. and Alt, F.W. 1986. Regulation of the assembly and expression of variable-region genes. Annu. Rev. Immunol. 4: 339-368.
28. Yancopoulos, G.D., Desiderio, S.V., Paskind, M., Kearney, J.F., Baltimore, D., and Alt, F.W. 1984. Preferential utilization of the most JH-proximal VH gene segments in pre-B-cell lines. Nature 311: 727-733.