Electron and atomic force microscopy of the trimeric ammonium transporter AmtB

EMBO Reports

Volumn 5, Issue 12, 2004, Pages 1153-1158

  Conroy, Matthew J ^a   Jamieson, Stuart J ^a,d   Blakey, Daniel ^b,e   Kaufmann, Thomas ^c   Engel, Andreas ^c   Fotiadis, Dimitrios ^c   Merrick, Mike ^b   Bullough, Per A ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

^b JOHN INNES CENTRE   (United Kingdom)

^c UNIVERSITY OF BASEL   (Switzerland)

^d Avecia Biotechnology   (United Kingdom)

^e UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Ammonium transport; Atomic force microscopy; Electron microscopy; Membrane protein; Projection structure

Indexed keywords

AMMONIA; CARRIER PROTEIN; LIPID; MONOMER; POLYPEPTIDE; PROTEIN AMTB; UNCLASSIFIED DRUG;

ARTICLE; ATOMIC FORCE MICROSCOPY; CELL MEMBRANE; CONTROLLED STUDY; CRYOELECTRON MICROSCOPY; CRYSTAL STRUCTURE; CRYSTALLIZATION; ESCHERICHIA COLI; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; STRUCTURE ANALYSIS;

CATION TRANSPORT PROTEINS; CRYOELECTRON MICROSCOPY; CRYSTALLIZATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; MICROSCOPY, ATOMIC FORCE;

ESCHERICHIA COLI;

EID: 12144264392     PISSN: 1469221X     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.embor.7400296     Document Type: Article

References (32)

1. Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S (2003) Structure and mechanism of the lactose permease of Escherichia coli. Science 301: 610-615
2. Bass RB, Strop P, Barclay M, Rees DC (2002) Crystal structure of Escherichia coli MscS, a voltage-modulated and mechanosensitive channel. Science 298: 1582-1587
3. Blakey D, Leech A, Thomas GH, Coutts G, Findlay K, Merrick M (2002) Purification of the Escherichia coli ammonium transporter AmtB reveals a trimeric stoichiometry. Biochem J 364: 527-535
4. Chang G, Spencer RH, Lee AT, Barclay MT, Rees DC (1998) Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel. Science 282: 2220-2226
5. Coutts G, Thomas G, Blakey D, Merrick M (2002) Membrane sequestration of the signal transduction protein GInK by the ammonium transporter AmtB. EMBO J 21: 1-10
6. Crowther RA, Henderson R, Smith JM (1996) MRC image processing programs. J Struct Biol 116: 9-16
7. + conduction and selectivity. Science 280: 69-77
8. Dutzler R, Campbell EB, Cadene M, Chait BT, MacKinnon R (2002) X-ray structure of a CIC chloride channel a 3.0Å reveals the molecular basis of anion selectivity. Nature 415: 287-294
9. Eyers SA, Ridgwell K, Mawby WJ, Tanner MJ (1994) Topology and organization of human Rh (rhesus) blood group-related polypeptides. J Biol Chem 269: 6417-6423
10. Henderson R, Baldwin JM, Downing KH, Lepault J, Zemlin F (1986) Structure of purple membrane from Halobacterium halobium - recording, measurement and evaluation of electron-micrographs at 3.5 Å resolution. Ultramicroscopy 19: 147-178
11. Jap BK, Zulauf M, Scheybani T, Hefti A, Baumeister W, Aebi U, Engel A (1992) 2D crystallization: from art to science. Ultramicroscopy 46: 45-84
12. Javelle A, Severi E, Thornton J, Merrick M (2004) Ammonium sensing in E. coli: the role of the ammonium transporter AmtB and AmtB-GInK complex formation. J Biol Chem 279: 8530-8538
13. +-uniporters. J Biol Chem 278: 45603-45610
14. Marini A-M, Andre B (2000) In vivo N-glycosylation of the Mep2 high-affinity ammonium transporter of Saccharomyces cerevisiae reveals an extracytosolic N-terminus. Mol Microbiol 38: 552-564
15. Marini A-M, Matassi G, Raynal V, Andre B, Cartron J, Cherif-Zahar B (2000) The human Rhesus-associated RhAG protein and a kidney homologue promote ammonium transport in yeast. Nat Genet 26: 341-344
16. Mindell JA, Maduke M, Miller C, Grigorieff N (2001) Projection structure of a CIC-type chloride channel at 6.5 Å resolution. Nature 409: 219-223
17. Monahan BJ, Unkles SE, Tsing IT, Kinghorn JR, Hynes MJ, Davis MA (2002) Mutation and functional analysis of the Aspergillus nidulans ammonium permease MeaA and evidence for interaction with itself and MepA. Fungal Genet Biol 36: 35-46
18. Murakami S, Nakashima R, Yamashita E, Yamaguchi A (2002) Crystal structure of bacterial multidrug efflux transporter AcrB. Nature 419: 587-593
19. Quentin F, Eladari D, Cheval L, Lopez C, Goossens D, Colin Y, Cartron JP, Paillard M, Chambrey R (2003) RhBG and RhCG, the putative ammonia transporters, are expressed in the same cells in the distal nephron. J Am Soc Nephrol 14: 545-554
20. Saxton WO (1996) SEMPER: distortion compensation, selective averaging, 3-D reconstruction, and transfer function correction in a highly programmable system. J Struct Biol 116: 230-236
21. Schertler GF, Villa C, Henderson R (1993) Projection structure of rhodopsin. Nature 362: 770-772
22. Thomas GH, Mullins JG, Merrick M (2000) Membrane topology of the Mep/Amt family of ammonium transporters. Mol Microbiol 37 331-344
23. Valpuesta JM, Carrascosa JL, Henderson R (1994) Analysis of electron microscope images and electron diffraction patterns of thin crystals of phi 29 connectors in ice. J Mol Biol 240: 281-287
24. Van den Berg B, Clemons WMJ, Collinson I, Modis Y, Hartmann E, Harrison SC, Rapoport TA (2004) X-ray structure of a protein-conducting channel. Nature 427: 36-44
25. van Heel M, Harauz G, Orlova EV (1996) A new generation of the IMAGIC image processing system. J Struct Biol 116: 17-24
26. Veenhoff LM, Heuberger EHML, Poolman B (2002) Quaternary structure and function of transport proteins. Trends Biochem Sci 27: 242-249
27. von Wirén N, Merrick M (2004) Regulation and function of ammonium carriers in bacteria, fungi and plants. Top Curr Genet 9: 95-120
28. von Wirén N, Lauter FR, Ninnemann O, Gillissen B, Walch-Liu P, Engels C, Jost W, Frommer WB (2000) Differential regulation of three functional ammonium transporter genes by nitrogen in root hairs and by light in leaves of tomato. Plant J 21: 167-175
29. Westhoff CM, Ferreri-Jacobia M, Mak DO, Foskett JK (2002) Identification of the erythrocyte Rh-blood group glycoprotein as a mammalian ammonium transporter. J Biol Chem 277: 12499-12502
30. Xu Y, Cheah E, Carr PD, van Heeswijk WC, Westerhoff HV, Vasudevan SG, Ollis DL (1998) GInK, a PII-homologue: structure reveals ATP binding site and indicates how the T-loops may be involved in molecular recognition. J Mol Biol 282: 149-165
31. Yin CC, Aldema-Ramos ML, Borges-Walmsley MI, Taylor RW, Walmsley AR, Levy SB, Bullough PA (2000) The quaternary molecular architecture of TetA, a secondary tetracycline transporter from Escherichia coli. Mol Microbiol 38: 482-492
32. Ziegler C, Morbach S, Schiller D, Kramer R, Tziatzios C, Schubert D, Kuhlbrandt W (2004) Projection structure and oligomeric state of the osmoregulated sodium/glycine betaine symporter BetP of Corynebacterium glutamicum. J Mol Biol 337: 1137-1147