Intracellular microrheology as a tool for the measurement of the local mechanical properties of live cells

Methods in Cell Biology

Volumn 2004, Issue 78, 2004, Pages 45-64

  Kole, Thomas P ^a   Tseng, Yiider ^a   Wirtz, Denis ^a,b  

^a Johns Hopkins University   (United States)

^b JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NANOTUBE;

ANIMAL; ARTICLE; BIOMECHANICS; CELL FUNCTION; CELL STRAIN 3T3; ELASTICITY; FLOW KINETICS; INSTRUMENTATION; INTERMEDIATE FILAMENT; METHODOLOGY; MICROINJECTION; MICROSCOPY; MOUSE; NASA DISCIPLINE CELL BIOTECHNOLOGY; NON-NASA CENTER; PHYSIOLOGY; STATISTICS; VISCOSITY;

NASA DISCIPLINE CELL BIOTECHNOLOGY; NON-NASA CENTER;

ANIMALS; BIOMECHANICS; CELL PHYSIOLOGY; ELASTICITY; INTERMEDIATE FILAMENTS; MICE; MICROINJECTIONS; MICROSCOPY, VIDEO; NANOTUBES; RHEOLOGY; SWISS 3T3 CELLS; VISCOSITY;

EID: 11144294550     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/s0091-679x(04)78003-6     Document Type: Review

References (57)

1. Apgar, J., Tseng, Y., Federov, E., Herwig, M. B., Almo, S. C., and Wirtz, D. (2000). Multiple-particle tracking measurements of heterogeneities in solutions of actin filaments and actin bundles. Biophys. J. 79, 1095-1106.
2. Bausch, A. R., Möller, W., and Sackmann, E. (1999). Measurement of local viscoelasticity and forces in living cells by magnetic tweezers. Biophys. J. 76, 573-579.
3. Beil, M., Micoulet, A., von Wiehert, G., Paschke, S., Walther, P., Omary, M. B., Van Veldhoven, P. P., Gern, U., Wolff-Hieber, E., Eggermann, J., Waltenberger, J., Adler, G., Spatz, J., and Seufferlein, T. (2003). Sphingosylphosphorylcholine regulates keratin network architecture and visco-elastic properties of human cancer cells. Nat. Cell. Biol. 5, 803-811.
4. Berg, H. C. (1993). "Random Walks in Biology." Princeton University Press, Princeton, N.J.
5. Borisy, G. G., and Svitkina, T. M. (2000). Actin machinery: Pushing the envelope. Cur. Opin. Cell Biol. 12, 104-112.
6. Cai, S., Pestic-Dragovich, L., O'Donnell, M. E., Wang, N., Ingber, D., Elson, E., and De Lanerolle, R. (1998). Regulation of cytoskeletal mechanics and cell growth by myosin light chain phosphorylation. Am. J. Physiol. 275, C1349-C1356.
7. Chandrasekhar, S. (1943). Stochastic problems in physics and astronomy. Rev. Mod. Phys. 15, 1-89.
8. Coulombe, P. A., Bousquet, O., Ma, L., Yamada, S., and Wirtz, D. (2000). The 'ins' and outs' of intermediate filament organization. Trends Cell. Biol. 10, 420-428.
9. Crick, F. H. C., and Hughes, A. F. W. (1950). The physical properties of cytoplasm: A study by means of the magnetic particle method part I. Exp. Cell Res. 1, 37-80.
10. Eckstein, A., Suhm, J., Friedrich, C., Maier, R. D., Sassmannshausen, J., Bochmann, M., and Mulhaupt, R. (1998). Determination of plateau moduli and entanglement molecular weights of isotactic, syndiotactic, and atactic polypropylenes synthesized with metallocene catalysts. Macromolecules 31, 1335-1340.
11. Einstein, A. (1905). Über die von der molekularkinetischen Theorie der Wärme geforderte Bewegung von in ruhenden Flüssigkeiten suspendierten Teilchen. Ann. Physik. 17, 549.
12. Ferry, J. D. (1980). "Viscoelastic Properties of Polymers." John Wiley & Sons, New York.
13. Gittes, F., Mickey, B., Nettleton, J., and Howard, J. (1993). Flexural rigidity of microtubules and Actin filaments measured from thermal fluctuations in shape. J. Cell Biol. 120, 923-934.
14. Gittes, F., Schnurr, B., Olmsted, P. D., MacKintosh, F. C., and Schmidt, C. F. (1997). Microscopic viscoelasticity: Shear moduli of soft materials determined from thermal fluctuations. Phys. Rev. Lett. 79, 3286-3289.
15. Goodman, A., Tseng, Y., and Wirtz, D. (2002). Effect of length, topology, and concentration on the microviscosity and microheterogeneity of DNA solutions. J. Mol. Biol. 323, 199-215.
16. Haga, H., Nagayama, M., Kawabata, K., Ito, E., Ushiki, T., and Sambongi, T. (2000). Time-lapse viscoelastic imaging of living fibroblasts using force modulation mode in AFM. J. Electron. Microsc. (Tokyo) 49, 473-481.
17. Heidemann, S. R., Kaech, S., Buxbaum, R. E., and Malus, A. (1999). Direct observations of the mechanical behaviors of the cytoskeleton in living fibroblasts. J. Cell Biol. 145, 109-122.
18. Heidemann, S. R., and Wirtz, D. (2004). Towards a regional approach to cell mechanics. Trends Cell Biol. 14, 160-166.
19. Hinner, B., Tempel, M., Sackmann, E., Kroy, K., and Frey, E. (1998). Entanglement, elasticity, and viscous relaxation of actin solutions. Phys. Rev. Lett. 81, 2614-2617.
20. Howard, J. (2001). "Mechanics of Motor Proteins and the Cytoskeleton." Sinauer Associates, Sunderland, Mass.
21. Janmey, P. A., Euteneuer, U., Traub, P., and Schliwa, M. (1991). Viscoelastic properties of vimentin compared with other filamentous biopolymer networks. J. Cell Biol. 113, 155-160.
22. Kole, T. P., Tseng, Y., Huang, L., Katz, J. L., and Wirtz, D. (2004). Rho kinase regulates the intracellular micromechanical response of adherent cells to Rho activation. Mol. Cell Biol. 15, 3475-3484.
23. Ma, L., Xu, J., Coulombe, P. A., and Wirtz, D. (1999). Epidermal keratin suspensions have unique micromechanical properties. J. Biol. Chem. 274, 19145-19151.
24. Ma, L., Yamada, S., Wirtz, D., and Coulombe, P. A. (2001). A hot-spot mutation alters the mechanical properties of keratin filament networks. Nat. Cell Biol. 3, 503-506.
25. Mason, T. G. (2000). Estimating the viscoelastic moduli of complex fluids using the generalized Stokes-Einstein equation. Rheologica Acta. 39, 371-378.
26. Mason, T. G., Dhople, A., and Wirtz, D. (1997a). Concentrated DNA rheology and microrheology. In "Statistical Mechanics in Physics and Biology," (D. Wirtz and T. C. Halsey, eds.), Vol. 463, pp. 153-158. Warrendale, Penn.
27. Mason, T. G., Ganesan, K., van Zanten, J. V., Wirtz, D., and Kuo, S. C. (1997b). Particle-tracking microrheology of complex fluids. Phys. Rev. Lett. 79, 3282-3285.
28. Merkel, R., Simson, R., Simson, D. A., Hohenadl, M., Boulbitch, A., Wallraff, E., and Sackmann, E. (2000). A micromechanic study of cell polarity and plasma membrane cell body coupling in Dictyostelium. Biophys. J. 79, 707-719.
29. Morse, D. C. (1998). Viscoelasticity of concentrated isotropic solutions of semiflexible polymers. 2. Linear response. Macromolecules 31, 7044-7067.
30. Mucke, N., Kreplak, L., Kirmse, R., Wedig, T., Herrmann, H., Aebi, U., and Langowski, J. (2004). Assessing the flexibility of intermediate filaments by atomic force microscopy. J. Mol. Biol. 335, 1241-1250.
31. Nagayama, M., Haga, H., and Kawabata, K. (2001). Drastic change of local stiffness distribution correlating to cell migration in living fibroblasts. Cell Motil. Cytoskeleton 50, 173-179.
32. Palmer, A., Xu, J., Kuo, S. C., and Wirtz, D. (1999). Diffusing wave spectroscopy microrheology of actin filament networks. Biophys. J. 76, 1063-1071.
33. Palmer, A., Xu, J., and Wirtz, D. (1998). High-frequency rheology of crosslinked actin networks measured by diffusing wave spectroscopy. Rheologica Acta. 37, 97-108.
34. Paulitschke, M., Nash, G. B., Anstee, D. J., Tanner, M. J., and Gratzer, W. B. (1995). Perturbation of red blood cell membrane rigidity by extracellular ligands. Blood 86, 342-348.
35. Petka, W. A., Harden, J., McGrath, K. P., Wirtz, D., and Tirrell, D. A. (1998). Reversible hydrogels from self-assembling artificial proteins. Science 281, 389-393.
36. Pollard, T. D., Almo, S., Quirk, S., Vinson, V., and Lattman, E. E. (1994). Structure of actin binding proteins: Insights about function at atomic resolution. Annu. Rev. Cell Biol. 10, 207-249.
37. Qian, H., Sheetz, M. P., and Elson, E. L. (1991). Single particle tracking. Analysis of diffusion and flow in two-dimensional systems. Biophys. J. 60, 910-921.
38. Ragsdale, G. K., Phelps, J., and Luby-Phelps, K. (1997). Viscoelastic response of fibroblasts to tension transmitted through adherens junctions. Biophys. J. 73, 2798-2808.
39. Rahman, A., Tseng, Y., and Wirtz, D. (2002). Micromechanical coupling between cell surface receptors and RGD peptides. Biochem. Biophys. Res. Commun. 296, 771-778.
40. Sato, M., Leimbach, G., Schwarz, W. H., and Pollard, T. D. (1985). Mechanical properties of actin. J. Biol. Chem. 260, 8585-8592.
41. Sato, M., Schwarz, W. H., and Pollard, T. D. (1987). Dependence of the mechanical properties of actin/α-actinin gels on deformation rate. Nature 325, 828-830.
42. Schmidt, A., and Hall, M. N. (1998). Signaling to the actin cytoskeleton. Annu. Rev. Cell Dev. Biol. 14, 305-338.
43. Schoenenberger, C. A., Steinmetz, M. O., Stoffler, D., Mandinova, A., and Aebi, U. (1999). Structure, assembly, and dynamics of actin filaments in situ and in vitro. Microsc. Res. Tech. 47, 38-50.
44. Steinmetz, M. O., Stoffler, D., Hoenger, A., Bremer, A., and Aebi, U. (1997). Actin: From cell biology to atomic detail. J. Struct. Biol. 119, 295-320.
45. Suh, J., Wirtz, D., and Hanes, J. (2003). Efficient active transport of gene nanocarriers to the cell nucleus. Proc. Natl. Acad. Sci. U.S.A 100, 3878-3882.
46. Svitkina, T. M., and Borisy, G. G. (1998). Correlative light and electron microscopy of the cytoskeleton of cultured cells. Methods Enzymol 298, 570-592.
47. Svitkina, T. M., and Borisy, G. G. (1999). Arp2/3 complex and actin depolymerizing factor cofilin in dendritic organization and treadmilling of actin filament array in lamellipodia. J. Cell Biol. 145, 1009-1026.
48. Thoumine, O., and Ott, A. (1997). Time scale dependent viscoelastic and contractile regimes in fibroblasts probed by microplate manipulation. J. Cell Sci. 110, 2109-2116.
49. Tseng, Y., Kole, T. P., and Wirtz, D. (2002). Micromechanical mapping of live cells by multiple-particle-tracking microrheology. Biophys. J. 83, 3162-3176.
50. Tseng, Y., Lee, J. S., Kole, T. P., Jiang, I., and Wirtz, D. (2004). Micro-organization and viscoelasticity of the interphase nucleus revealed by particle nanotracking. J. Cell Sci. 117, 2159-2167.
51. Tseng, Y., and Wirtz, D. (2001). Mechanics and multiple-particle tracking microhetrogeneity of alpha-actinin-cross-linked actin filament networks. Biophys. J. 81, 1643-1656.
52. Wang, N., Butler, J. P., and Ingber, D. E. (1993). Mechanotransduction across the cell surface and through the cytoskeleton. Science 260, 1124-1127.
53. Xu, J., Tseng, Y., Carriere, C. J., and Wirtz, D. (2002). Microrheology and microheterogeneity of wheat gliadin suspensions studied by multiple particle tracking. Biomacromolecules 3, 92-99.
54. Xu, J., Viasnoff, V., and Wirtz, D. (1998). Compliance of actin filament networks measured by particle-tracking microrheology and diffusing wave spectroscopy. Rheologica Acta. 37, 387-398.
55. Yamada, S., Wirtz, D., and Coulombe, P. A. (2002). Pairwise assembly determines the intrinsic potential for self-organization and mechanical properties of keratin filaments. Mol. Biol. Cell. 13, 382-391.
56. Yamada, S., Wirtz, D., and Coulombe, P. A. (2003). The mechanical properties of simple epithelial keratins 8 and 18: Discriminating between interfacial and bulk elasticities. J. Struct. Biol. 143, 45-55.
57. Yamada, S., Wirtz, D., and Kuo, S. C. (2000). Mechanics of living cells measured by laser tracking microrheology. Biophys. J. 78, 1736-1747.