Knockout of the high-coupling cytochrome aa3 oxidase reduces TCA cycle fluxes in Bacillus subtilis

FEMS Microbiology Letters

Volumn 226, Issue 1, 2003, Pages 121-126

  Zamboni, Nicola ^a   Sauer, Uwe ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Bioenergetics; Metabolic engineering; Metabolic flux analysis; Metabolic flux ratio analysis; Respiration; Tricarboxylic acid cycle

Indexed keywords

ADENOSINE TRIPHOSPHATE; CARBON 13; CITRIC ACID; CYTOCHROME C OXIDASE; MUTANT PROTEIN; ACETIC ACID DERIVATIVE; CYTOCHROME; GLUCOSE; MULTIENZYME COMPLEX; OXIDOREDUCTASE;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GROWTH; BACTERIUM CULTURE; BIOENERGY; CITRIC ACID CYCLE; METABOLISM; MICROBIAL BIOMASS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REDUCTION; BACTERIAL GENE; BIOMASS; BIOSYNTHESIS; ELECTRON TRANSPORT; GENETICS; GLUCONEOGENESIS; GROWTH, DEVELOPMENT AND AGING; MASS FRAGMENTOGRAPHY; MUTATION; PENTOSE PHOSPHATE CYCLE; PHYSIOLOGY;

BACILLUS SUBTILIS;

ACETATES; ADENOSINE TRIPHOSPHATE; BACILLUS SUBTILIS; BIOMASS; CITRIC ACID CYCLE; CYTOCHROMES; ELECTRON TRANSPORT; ELECTRON TRANSPORT CHAIN COMPLEX PROTEINS; ELECTRON TRANSPORT COMPLEX IV; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GENES, BACTERIAL; GLUCONEOGENESIS; GLUCOSE; MUTATION; OXIDOREDUCTASES; PENTOSE PHOSPHATE PATHWAY;

EID: 0642369576     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00614-1     Document Type: Article

References (33)

1. Helling R.B. Speed versus efficiency in microbial growth and the role of parallel pathways. J. Bacteriol. 184:2002;1041-1045.
2. Pfeiffer T., Schuster S., Bonhoeffer S. Cooperation and competition in the evolution of ATP-producing pathways. Science. 292:2001;504-507.
3. Dauner M., Storni T., Sauer U. Bacillus subtilis metabolism and energetics in carbon-limited and excess-carbon chemostat culture. J. Bacteriol. 183:2001;7308-7317.
4. Neijssel O.M., Teixeira de Mattos M.J. The energetics of bacterial growth: a reassessment. Mol. Microbiol. 13:1994;172-182.
5. 3 or cytochrome bd, is required for aerobic growth. J. Bacteriol. 182:2000;6557-6564.
6. Trumpower B.L., Gennis R.B. Energy transduction by cytochrome complexes in mitochondrial and bacterial respiration: The enzymology of coupling electron transfer reactions to transmembrane proton translocation. Annu. Rev. Biochem. 63:1994;675-716.
7. Zamboni N., Mouncey N., Hohmann H.P., Sauer U. Reducing maintenance metabolism by metabolic engineering of respiration improves riboflavin production by Bacillus subtilis. Metab. Eng. 5:2003;49-55.
8. Lauraeus M., Wikström M. The terminal quinol oxidases of Bacillus subtilis have different energy conservation properties. J. Biol. Chem. 268:1993;11470-11473.
9. 3-600 quinol oxidase. J. Biol. Chem. 267:1992;10225-10231.
10. Winstedt L., Yoshida K., Fujita Y., von Wachenfeldt C. Cytochrome bd biosynthesis in Bacillus subtilis: characterization of the cydABCD operon. J. Bacteriol. 180:1998;6571-6580.
11. Saraste M. Oxidative phosphorylation at the fin de siecle. Science. 283:1999;1488-1493.
12. 0-ATP synthases: glimpses of interacting parts in a dynamic molecular machine. J. Exp. Biol. 200:1997;217-224.
13. 1 ATP synthase from thermophilic Bacillus PS3 reconstituted into liposomes with bacteriorhodopsin. 2. Relationships between proton motive force and ATP synthesis. Eur. J. Biochem. 235:1996;779-788.
14. Sauer U., Bailey J.E. Estimation of P-to-O ratio in Bacillus subtilis and its influence on maximum riboflavin yield. Biotechnol. Bioeng. 64:1999;750-754.
15. Perkins J.B., et al. Genetic engineering of Bacillus subtilis for the commercial production of riboflavin. J. Ind. Microbiol. Biotechnol. 22:1999;8-18.
16. 13C- labeling of proteinogenic amino acids. An efficient analytical tool to investigate intermediary metabolism. Eur. J. Biochem. 232:1995;433-448.
17. 13C-NMR, MS and metabolic flux balancing in biotechnology research. Q. Rev. Biophys. 31:1998;41-106.
18. Sauer U., Hatzimanikatis V., Bailey J.E., Hochuli M., Szyperski T., Wüthrich K. Metabolic fluxes in riboflavin-producing Bacillus subtilis. Nat. Biotechnol. 15:1997;448-452.
19. Fischer E., Sauer U. Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC-MS. Eur. J. Biochem. 270:2003;880-891.
20. Dauner M., Sauer U. GC-MS analysis of amino acids rapidly provides rich information for isotopomer balancing. Biotechnol. Prog. 16:2000;642-649.
21. Christensen B., Nielsen J. Isotopomer analysis using GC-MS. Metab. Eng. 1:1999;282-290.
22. Wittmann C., Heinzle E. Mass spectrometry for metabolic flux analysis. Biotechnol. Bioeng. 62:1999;739-750.
23. Harwood, C.R. and Cutting, S.M. (1990) in: Modern Microbiological Methods. John Wiley and Sons, Chichester.
24. Sauer U., Lasko D.R., Fiaux J., Hochuli M., Glaser R., Szyperski T., Wüthrich K., Bailey J.E. Metabolic flux ratio analysis of genetic and environmental modulations of Escherichia coli central carbon metabolism. J. Bacteriol. 181:1999;6679-6688.
25. Dauner M., Sauer U. Stoichiometric growth model for riboflavin-producing Bacillus subtilis. Biotechnol. Bioeng. 76:2001;132-143.
26. Bonarius H.P.J., Schmid G., Tramper J. Flux analysis of underdetermined metabolic networks: The quest for the missing constraints. Trends Biotechnol. 15:1997;308-314.
27. van der Heijden R.T.J.M., Heijnen J.J., Hellinga C., Romein B., Luyben K.C.A.M. Linear constraint relations in biochemical reaction systems: I. Classificatiopn of the calculability and the balanceability of conversion rates. Biotechnol. Bioeng. 43:1994;3-10.
28. Klamt S., Schuster S., Gilles E.D. Calculability analysis in underdetermined metabolic networks illustrated by a model of the central metabolism in purple nonsulfur bacteria. Biotechnol. Bioeng. 77:2002;734-751.
29. Sauer U., Hatzimanikatis V., Hohmann H.-P., Manneberg M., van Loon A.P., Bailey J.E. Physiology and metabolic fluxes of wild-type and riboflavin-producing Bacillus subtilis. Appl. Environ. Microbiol. 62:1996;3687-3696.
30. Fry B., Zhu T., Domach M.M., Koepsel R.R., Phalakornkule C., Ataai M.M. Characterization of growth and acid formation in a Bacillus subtilis pyruvate kinase mutant. Appl. Environ. Microbiol. 66:2000;4045-4049.
31. Majewski R.A., Domach M.M. Simple constrained-optimization view of acetate overflow in Escherichia coli. Biotechnol. Bioeng. 35:1990;732-738.
32. Han K., Lim H.C., Hong J. Acetic-acid formation in Escherichia coli fermentation. Biotechnol. Bioeng. 39:1992;663-671.
33. el-Mansi E.M., Holms W.H. Control of carbon flux to acetate excretion during growth of Escherichia coli in batch and continuous cultures. J. Gen. Microbiol. 135:1989;2875-2883.