Expression of the p16INK4a Gene and Methylation Pattern of CpG Sites in the Promoter Region in Rat Tumor Cell Lines

Molecular Carcinogenesis

Volumn 39, Issue 1, 2004, Pages 10-14

  Honoki, Kanya ^a   Tsujiuchi, Toshifumi ^a   Mori, Toshio ^a   Yoshitani, Kazuhiro ^a   Tsutsumi, Masahiro ^a   Takakura, Yoshinori ^a   Mii, Yoshio ^a  

^a NARA MEDICAL UNIVERSITY   (Japan)

Author keywords

Gene silencing; Methylation; p16INK4a; Rat; TATA box

Indexed keywords

5 AZA 2' DEOXYCYTIDINE; PROTEIN P16; AZACITIDINE; BISULFITE; DRUG DERIVATIVE; MESSENGER RNA; PROTEIN P16INK4A; SULFITE;

ANIMAL CELL; ARTICLE; CANCER; CPG ISLAND; DNA METHYLATION; EXON; FIBROBLAST; GENE EXPRESSION; GENE SILENCING; LUNG ADENOCARCINOMA; MALIGNANT FIBROUS HISTIOCYTOMA; NONHUMAN; OSTEOSARCOMA CELL; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; RAT; TATA BOX; TUMOR CELL LINE; ADENOCARCINOMA; ANIMAL; CELL CULTURE; DERMATOFIBROMA; GENETICS; LUNG TUMOR; METABOLISM; NEOPLASM; OSTEOSARCOMA; PATHOLOGY;

ADENOCARCINOMA; ANIMALS; AZACITIDINE; CPG ISLANDS; CYCLIN-DEPENDENT KINASE INHIBITOR P16; DNA METHYLATION; FIBROBLASTS; GENE SILENCING; HISTIOCYTOMA, BENIGN FIBROUS; LUNG NEOPLASMS; NEOPLASMS; OSTEOSARCOMA; PROMOTER REGIONS (GENETICS); RATS; RNA, MESSENGER; SULFITES; TATA BOX; TUMOR CELLS, CULTURED;

EID: 0347357849     PISSN: 08991987     EISSN: None     Source Type: Journal    
DOI: 10.1002/mc.10165     Document Type: Article

References (22)

1. Meyerson M, Enders GH, Wu CL, et al. A family of human cdc2-related protein kinases. EMBO J 1992;11:2909-2917.
2. 1 cyclin-dependent kinases. Genes Dev 1995;9:1149-1163.
3. Ruas M, Peters G. The p161NK4A/CDKN2 tumor suppressor and its relatives. Biochem Biophys Acta 1998;1378:F115-F177.
4. INK4a protein is restricted to the subset of lung cancer lines that retains wildtype Rb. Oncogene 1994;9:3375-3378.
5. INK4a is an early event in lung cancer and a potential biomarker for early diagnosis. Proc Natl Acd Sci USA 1998;5:11891-11896.
6. Wei G, Lonardo F, Ueda T, et al. CDK4 gene amplification in osteosarcoma: Reciprocal relationship with INK4A gene alteration and mapping of 12q13 amplicons. Int J Cancer 1999;80:199-204.
7. Benassi MS, Molendini L, Gamberi G, et al. Alteration of pRb/p16/cdk4 regulation in human osteosarcoma. Int J Cancer 1999;84:489-493.
8. Herman JG, Merlo A, Mao L, et al. Inactivation of the CDKN2/p16/MTS1 gene is frequently associated with aberrant DNA methylation in all common human cancers. Cancer Res 1995;55:4525-4530.
9. INK4a and β-catenin alterations in rat liver tumors induced by NNK. Carcinogenesis 2001;22:461-466.
10. INK4A tumor suppressor genes in oxystress-induced renal cell carcinoma of rats. Oncogene 1999;18: 3793-3797.
11. Herman JG, Baylin SB. Promoter-region hypermethylation and gene silencing in human cancer. Curr Top Microbiol Immunol 2000;249:35-54.
12. Abe M, Okochi E, Kuramoto T, et al. Cloning of the 5′ upstream region of the rat p16 gene and its role in silencing. Jpn J Cancer Res 2002;93:1100-1106.
13. Chan MF, Liang G, Jones PA. Relationship between transcription and DNA methylation. Curr Top Microbiol Immunol 2000;249:75-86.
14. Pfeifer GP, Steigerwald SD, Hansen RS, Gartler SM, Riggs AD. Polymerase chain reaction-aided genomic sequencing of an X chromosome-linked CpG island: Methylation patterns suggest clonal inheritance, CpG site autonomy, and an explanation of activity state stability. Proc Natl Acad Sci USA 1990;87:8252-8256.
15. Levine A, Cantoni GL, Razin A. Methylation in the preinitiation domain suppresses gene transcription by an indirect mechanism. Pro Natl Acad Sci USA 1992;89: 10119-10123.
16. Gonzalgo ML, Hayashida T, Bender CM, et al. The role of DNA methylation in expression of the p19/p16 locus in human bladder cancer cell lines. Cancer Res 1998;58:1245-1252.
17. Tsujiuchi T, Tsutsumi M, Sasaki Y, Murata N, Konishi Y. Mutations of adenomatous polyposis coli and β-catenin genes during progression of lung tumors induce by N-nitrosobis (2-hydroxypropyl) amine in rats. Cancer Res 2000; 60:6611-6616.
18. Honoki K, Tsutsumi M, Miyauchi Y, et al. Increased expression of nucleoside diphosphate kinase/nm23 and c-Ha-ras mRNA is associated with spontaneous lung metastasis in rat-transplantable osteosarcomas. Cancer Res 1993;53: 5038-5042.
19. Honoki K, Tsujiuchi T, Sasaki Y, et al. Differential expression of cytokines in rat osteosarcoma and malignant fibrous histiocytoma cell lines induced by 4-(hydroxyamino) quinoline-1-oxide. Mol Carcinogenesis 2002;33:81-87.
20. Konishi Y, Maruyama H, Mii Y, Miyauchi Y, Yokose Y, Masuhara K. Malignant fibrous histiocytomas induced by 4-(hydroxyamino) quinoline-1-oxide in rats. J Natl Cancer Inst 1982;68:859-862.
21. Rein T, Zorbas H, Depaphilis ML. Active mammalian replication origins are associated with a high-density cluster of mCpG dinucleotides. Mol Cell Biol 1997;17:416-426.
22. Zhu W-G, Dai Z, Ding H, et al. Increased expression of unmethylated CDK2D by 5-aza-2′-deoxycytidine in human lung cancer cells. Oncogene 2001;20:7787-7796.