Exploring vaccinia virus as a tool for large-scale recombinant protein expression

Biotechnology Progress

Volumn 19, Issue 1, 2003, Pages 130-136

  Bleckwenn, Nicole A ^a,b   Bentley, William E ^b   Shiloach, Joseph ^a  

^a NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^b UNIVERSITY OF MARYLAND   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; FLUORESCENCE; PROTEINS; VIRUSES;

RECOMBINANT VACCINIA VIRUS; SERUM CONCENTRATION;

BIOTECHNOLOGY;

BIOREACTORS; FEASIBILITY STUDIES; GENE EXPRESSION REGULATION, VIRAL; GENE TRANSFER TECHNIQUES; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; LUMINESCENT PROTEINS; PILOT PROJECTS; PROTEIN ENGINEERING; QUALITY CONTROL; RECOMBINANT PROTEINS; VACCINIA VIRUS;

VACCINIA VIRUS;

EID: 0347297509     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp0255762     Document Type: Conference Paper

References (33)

1. Fang, N. X.; Frazer, I. H.; Fernando, G. J. P. Differences in the Post-Translational Modifications of Human Papilloma-virus Type 6b Major Capsid Protein Expressed from a Baculovirus System Compared with a Vaccinia Virus System. Biotechnol. Appl. Biochem. 2000, 32, 27-33.
2. Percival, M. D.; Bastien, L.; Griffin, P. R.; Kargman, S.; Ouellet, M.; O'Neill, G. P. Investigation of Human Cyclooxy-genase-2 Glycosylation Heterogeneity and Protein Expression in Insect and Mammalian Cell Expression Systems. Protein Expression Purif. 1997, 9, 388-398.
3. Ailor, E.; Takahashi, N.; Tsukamoto, Y.; Masuda, K.; Rahman, B. A.; Jarvis, D. L.; Lee, Y. C.; Betenbaugh, M. J. N-Glycan Patterns of Human Transferrin Produced in Trichoplusia ni Insect Cells: Effects of Mammalian Galactosyl-transferase. Glycobiology 2000, 10, 837-847.
4. Ailor, E.; Pathmanathan, J.; Jongbloed, J. D. H.; Betenbaugh, M. J. A Bacterial Signal Peptidase Enhances Processing of a Recombinant Single Chain Antibody Fragment in Insect Cells. Biochem. Biophys. Res. Commun. 1999, 255, 444-450.
5. Ailor, E.; Betenbaugh, M. J. Modifying Secretion and Post-Translational Processing in Insect Cells. Curr. Opin. Biotechnol. 1999, 10, 142-145.
6. Moss, B. Genetically Engineered Poxviruses for Recombinant Gene Expression, Vaccination, and Safety. Proc. Natl. Acad. Sci. U.S.A. 1996, 93, 11341-11348.
7. Broder, C. C.; Earl, P. L. Recombinant Vaccinia Viruses-Design, Generation, and Isolation. Mol. Biotechnol. 1999, 13, 223-245.
8. Sutter, G.; Moss, B. Nonreplicating Vaccinia Vector Efficiently Expresses Recombinant Genes. Proc. Natl. Acad. Sci. U.S.A. 1992, 89, 10847-10851.
9. Carroll, M. W.; Moss, B. Host Range and Cytopathogenicity of the Highly Attenuated MVA Strain of Vaccinia Virus: Propagation and Generation of Recombinant Viruses in a Nonhuman Mammalian Cell Line. Virology 1997, 238, 198-211.
10. Chalfie, M.; Tu, Y.; Euskirchen, G.; Ward, W. W.; Prasher, D. C. Green Fluorescent Protein as a Marker for Gene-Expression. Science 1994, 263, 802-805.
11. Albano, C. R.; Randers-Eichhorn, L.; Bentley, W. E.; Rao, G. Green Fluorescent Protein as a Real Time Quantitative Reporter of Heterologous Protein Production. Biotechnol. Prog. 1998, 14, 351-354.
12. Cha, H. J.; Wu, C. F.; Valdes, J. J.; Rao, G.; Bentley, W. E. Observations of Green Fluorescent Protein as a Fusion Partner in Genetically Engineered Escherichia coli: Monitoring Protein Expression and Solubility. Biotechnol. Bioeng. 2000, 67, 565-574.
13. DeLisa, M. P.; Li, J. C.; Rao, G.; Weigand, W. A.; Bentley, W. E. Monitoring GFP-Operon Fusion Protein Expression During High Cell Density Cultivation of Escherichia coli Using an On-Line Optical Sensor. Biotechnol. Bioeng. 1999, 65, 54-64.
14. Wu, C. F.; Cha, H. J.; Rao, G.; Valdes, J. J.; Bentley, W. E. A Green Fluorescent Protein Fusion Strategy for Monitoring the Expression, Cellular Location, and Separation of Biologically Active Organophosphorus Hydrolase. Appl. Microbiol. Biotechnol. 2000, 54, 78-83.
15. Li, J. C.; Wang, S.; VanDusen, W. J.; Schultz, L. D.; George, H. A.; Herber, W. K.; Chae, H. J.; Bentley, W. E.; Rao, G. Green Fluorescent Protein in Saccharomyces cerevisiae: Real-Time Studies of the GAL1 Promoter. Biotechnol. Bioeng. 2000, 70, 187-196.
16. Cha, H. J.; Dalal, N. G.; Vakharia, V. N.; Bentley, W. E. Expression and Purification of Human Interleukin-2 Simplified as a Fusion with Green Fluorescent Protein in Suspended Sf-9 Insect Cells. J. Biotechnol. 1999, 69, 9-17.
17. Laukkanen, M. L.; OkerBlom, C.; Keinanen, K. Secretion of Green Fluorescent Protein from Recombinant Baculovirus-Infected Insect Cells. Biochem. Biophys. Res. Commun. 1996, 226, 755-761.
18. Cha, H. J.; Dalal, N. G.; Pham, M. Q.; Vakharia, V. N.; Rao, G.; Bentley, W. E. Insect Larval Expression Process Is Optimized by Generating Fusions with Green Fluorescent Protein. Biotechnol. Bioeng. 1999, 65, 316-324.
19. Cha, H. J.; Pham, M. Q.; Rao, G.; Bentley, W. E. Expression of Green Fluorescent Protein in Insect Larvae and Its Application for Heterologous Protein Production. Biotechnol. Bioeng. 1997, 56, 239-247. Ward, G. A.; Stover, C. K.; Moss, B.; Fuerst, T. R. Stringent Chemical and Thermal Regulation of Recombinant Gene Expression by Vaccinia Virus Vectors in Mammalian Cells. Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 6773-6777.
20. Cha, H. J.; Pham, M. Q.; Rao, G.; Bentley, W. E. Expression of Green Fluorescent Protein in Insect Larvae and Its Application for Heterologous Protein Production. Biotechnol. Bioeng. 1997, 56, 239-247. Ward, G. A.; Stover, C. K.; Moss, B.; Fuerst, T. R. Stringent Chemical and Thermal Regulation of Recombinant Gene Expression by Vaccinia Virus Vectors in Mammalian Cells. Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 6773-6777.
21. Crameri, A.; Whitehorn, E. A.; Tate, E.; Stemmer, W. P. C. Improved Green Fluorescent Protein by Molecular Evolution Using DNA Shuffling. Nat. Biotechnol. 1996, 14, 315-319.
22. Ward, G. A.; Stover, C. K.; Moss, B.; Fuerst, T. R. Stringent Chemical and Thermal Regulation of Recombinant Gene Expression by Vaccinia Virus Vectors in Mammalian Cells. Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 6773-6777.
23. Barrett, N.; Mitterer, A.; Mundt, W.; Eibl, J.; Eibl, M.; Gallo, R. C.; Moss, B.; Dorner, F. Large-Scale Production and Purification of a Vaccinia Recombinant-Derived HIV-1 gp160 and Analysis of Its Immunogenicity. AIDS Res. Hum. Retroviruses 1989, 5, 159-171.
24. Hu, Y. C.; Kaufman, J.; Cho, M. W.; Golding, H.; Shiloach, J. Production of HIV-1 gp120 in Packed-Bed Bioreactor Using the Vaccinia Virus/T7 Expression System. Biotechnol. Prog. 2000, 16, 744-750.
25. Fuerst, T. R.; Niles, E. G.; Studier, F. W.; Moss, B. Eukaryotic Transient-Expression System Based on Recombinant Vaccinia Virus That Synthesizes Bacteriophage-T7 RNA-Polymerase. Proc. Natl. Acad. Sci. U.S.A. 1986, 83, 8122-8126.
26. Fuerst, T. R.; Earl, P. L.; Moss, B. Use of a Hybrid Vaccinia Virus-T7 RNA-Polymerase System for Expression of Target Genes. Mol. Cell. Biol. 1987, 7, 2538-2544.
27. Blasco, R.; Moss, B. Role of Cell-Associated Enveloped Vaccinia Virus in Cell-to-Cell Spread. J. Virol. 1992, 66, 4170-4179.
28. Sambanis, A.; Hu, W. S. Cell Culture Bioreactors. In Biotechnology: A Multi-Volume Comprehensive Treatise, 2nd ed.; Rehn, H. J., Reed, G., Eds.; John Wiley and Sons: New York, 1993; Vol. 3, pp 105-126.
29. Moss, B.; Earl, P. Expression of Proteins in Mammalian Cells Using Vaccinia Viral Vectors. In Current Protocols in Molecular Biology; John Wiley & Sons: Edison, NJ, 2000.
30. Christen, L.; Seto, J.; Niles, E. G. Superinfection Exclusion of Vaccinia Virus in Virus-Infected Cell-Cultures. Virology 1990, 174, 35-42.
31. Wang, M. Y.; Kwong, S.; Bentley, W. E. Effects of Oxygen Glucose Glutamine Feeding on Insect-Cell Baculovirus Protein Expression - A Study on Epoxide Hydrolase Production. Biotechnol. Prog. 1993, 9, 355-361.
32. Dee, K. U.; Shuler, M. L. Optimization of an Assay for Baculovirus Titer and Design of Regimens for the Synchronous Infection of Insect Cells. Biotechnol. Prog. 1997, 13, 14-24.
33. van Wezel, A. L. Tissue Culture: Methods and Applications; Academic Press Inc.: New York, 1973; pp 372-377.