Expression of green fluorescent protein in insect larvae and its application for heterologous protein production

Biotechnology and Bioengineering

Volumn 56, Issue 3, 1997, Pages 239-247

  Cha, Hyung Joon ^a,c   Pham, Minh Quan ^a,c   Rao, Govind ^a,b   Bentley, William E ^a,c  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^b UNIVERSITY OF MARYLAND BALTIMORE COUNTY   (United States)

^c UNIVERSITY OF MARYLAND   (United States)

Author keywords

Baculovirus; Green fluorescent protein; Insect larvae; Interleukin 2

Indexed keywords

BIOREACTORS; CELL CULTURE; CELLS; FLUORESCENCE; PROTEINS; VIRUSES;

BACULOVIRUS; GREEN FLUORESCENT PROTEIN; INSECT LARVAE; INTERLEUKIN;

BIOTECHNOLOGY;

BIOLOGICAL MARKER; GREEN FLUORESCENT PROTEIN; INTERLEUKIN 2; PROTEINASE;

ANIMAL CELL; ARTICLE; BACULOVIRUS; EXPRESSION VECTOR; INSECT; LARVA; NONHUMAN; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; VIRUS RECOMBINANT;

EID: 0012839365     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19971105)56:3<239::AID-BIT1>3.0.CO;2-K     Document Type: Article

References (51)

1. Becht, H., Muller, H., Muller, H. K. 1988. Comparative studies on structural and antigenic properties of two serotypes of infectious bursal disease virus. J. Gen. Virol. 69: 631-640.
2. Bentley, W. E., Davis, R. H., Kompala, D. S. 1991. Dynamics of induced CAT expression in E. coli. Biotechnol. Bioeng. 38: 749-760.
3. Bokman, S. H., Ward, W. W. 1981. Renaturation of Aequorea green-fluorescent protein. Biochem. Biophys. Res. Comm. 101: 1372-1380.
4. Bradley, M. S., Kucera, M., Loeb, M., Bennettova, B. 1989. Alterations in protease, protease inhibitors and ecdysone levels: A profile of stress in insects. Comp. Biochem. Physiol. 94: 85-89.
5. Cameron, I. R., Bishop, D. H. L. 1989. Insect cell culture technology in baculovirus expression system. TIBTECH 7: 66-70.
6. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W., Prasher, D. C. 1994. Green fluorescent protein as a marker for gene expression. Science 263: 802-805.
7. Chapman, R. F. 1969. The insects, structure and function, pp. 388-402. English University Publishers, London, UK.
8. Crameri, A., Whitehorn, E. A., Tate, E., Stemmer, W. P. C. 1996. Improved green fluorescent protein by molecular evolution using DNA shuffling. Nature Biotechnol. 14: 315-319.
9. Fraser, J. J. 1989. Expression of eucaryotic genes in insect cell cultures. In Vitro 25: 225-235.
10. Gillis, S., Ferm, M. M., Ou, W., Smith, K. A. 1978. T cell growth factor: Parameters of production and a quantitative microassay for activity. J. Immunol. 120: 2027-2032.
11. Goosen, M. F. A. 1991. Insect cell cultivation techniques for the production of high-valued products. Can. J. Chem. Eng. 69: 450-456.
12. Gottesman, S., Maurizi, M. R. 1992. Regulation by proteolysis: Energy-dependent proteases and their targets. Microbiol. Rev. 56: 592-621.
13. Harcum, S. W., Bentley, W. E. 1993. Detection, quantification, and characterization of protease in recombinant Escherichia coli. Biotechnol. Techniques 7: 441-447.
14. Heinlein, M., Epel, B. L., Padgett, H. S., Beachy, R. N. 1995. Interaction of tobamovirus movement proteins with the plant cytoskeleton. Science 270: 1983-1985.
15. Ikawa, M., Kominami, K., Yoshimura, Y., Tanaka, K., Nishimune, Y., Okabe, M. 1995. Green fluorescent protein as a marker in transgenic mice. Devel. Growth. Differ. 37: 455-459.
16. Inouye, S., Tsuji, F. I. 1994. Aequorea green fluorescent protein: Expression of the gene and fluorescent characteristics of the recombinant protein. FEBS Letters 341: 277-280.
17. Ishida, N., Tsujimoto, M., Kanaya, T., Shimamura, A., Tsuruoka, N., Kodama, S., Katayama, T., Oikawa, S., Matsui, M., Nakanishi, T., Kobayashi, J., Nakazato, H. 1994. Expression and characterization of human bone morphogenetic protein-2 in silkworm larvae infected with recombinant Bombyx mori nuclear polyhedrosis virus. J. Biochem. 115: 279-285.
18. Johnson, F. H., Shimomura, O., Saiga, Y., Gershman, L. C., Reynolds, G. T., Waters, J. R. 1962. Quantum efficiency of Cypridina luminescence, with a note on that of Aequorea. J. Cell. Comp. Physiol. 60: 85-103.
19. Kain, S. R., Adams, M., Kondepudi, A., Yang, T. T., Ward, W. W., Kitts, P. 1995. The green fluorescent protein as a reporter of gene expression and protein localization. Bio Techniques 17: 982-987.
20. King, L. A., Possee, R. D. 1992. The baculovirus expression system: A laboratory manual. Chapman and Hall, New York.
21. Kucera, M., Sehnal, F., Mala, J. 1984. Effect of developmental derangements on the proteolytic and protease-inhibitory activities in Galleria mellonella (insecta). Comp. Biochem. Physiol. 79: 255-261.
22. Kuroda, K., Groner, A., Frese, K., Drenckhahn, D., Hauser, C., Rott, R., Doerfler, W., Klenk, H.-D. 1989. Synthesis of biologically active influenza virus hemagglutinin in insect larvae. J. Virol. 63: 1677-1685.
23. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature (London) 227: 680-685.
24. Luckow, V. A., Summers, M. D. 1988. Trends in the development of baculovirus expression vectors. Bio/Technology 6: 47-55.
25. Maeda, S., Kawai, T., Obinata, M., Fujiwara, H., Horiuchi, T., Saeki, Y., Sato, Y., Furusawa, M. 1985. Production of human α-interferon in silkworm using a baculovirus vector. Nature 315: 592-594.
26. Marumoto, Y., Sato, Y., Fujiwara, H., Sakano, K., Sakei, Y., Agata, M., Furusawa, M., Maeda, S. 1987. Hyperproduction of polyhedrin-IGF II fusion protein in silkworm larvae infected with recombinant Bombyx mori nuclear polyhedrus virus. J. Gen. Virol. 68: 2599-2606.
27. Maurizi, M. R. 1992. Proteases and protein degradation in Escherichia coli. Experimentia. 48: 178-200.
28. Medin, J. A., Hunt, L., Gathy, K., Evans, R. K., Coleman, M. S. 1990. Efficient, low-cost protein factories: expression of human adenosine deaminase in baculovirus-infected insect larvae. Proc. Natl. Acad. Sci. USA 87: 2760-2764.
29. Miller, M. K. 1988. Baculoviruses as gene expression vectors. Annu. Rev. Microbiol. 42: 177-199.
30. Miyajima, A., Schreurs, J., Otsu, K., Kondo, A., Arai, K., Maeda, S. 1987. Use of the silkworm, Bombyx mori, and an insect baculovirus vector for high-level expression and secretion of biologically active mouse interleukin-3. Gene 58: 273-281.
31. Naggie, S., Pulliam, T. R., Bentley, W. E. 1996. Elicitation of protease activity in insect (Sf-9) cell culture. Abstracts of the American Chemical Society 211: 163.
32. Niedz, R. P., Sussman, M. R., Satterlee, J. S. 1995. Green fluorescent protein: An in vivo reporter of plant gene expression. Plant Cell Reports 14: 403-406.
33. Ohkawa, T., Majima, K., Maeda, S. 1994. A cystein protease encoded by the baculovirus Bombyx mori nuclear polyhedrosis virus. J. Virol. 68: 6619-6625.
34. O'Reilly, D. R., Miller, L. K., Luckow, V. A. 1992. Baculovirus expression vectors: A laboratory manual. Freeman, New York.
35. Palhan, V. B., Sumathy, S., Gopinathan, K. P. 1995. Baculovirus mediated high-level expression of luciferase in silkworm cells and larvae. BioTechniques 19: 97-104.
36. Pines, J. 1995. GFP in mammalian cells. Trends Genet. 11: 326-327.
37. Prasher, D. C., Eckenrode, V. K., Ward, W. W., Prendergast, F. G., Cormier, M. J. 1992. Primary structure of the Aequorea victoria green fluorescent protein. Gene 111: 229-233.
38. Price, P. M., Reichelderfer, C. F., Johansson, B. E., Kilbourne, E. D., Acs, G. 1989. Complementation of recombinant baculoviruses by coinfection with wild-type virus facilitates production in insect larvae of antigenic proteins of hepatitis B virus and influenza virus. Proc. Natl. Acad. Sci. USA 86: 1453-1456.
39. Randers-Eichhorn, L., Albano, C. R., Sipior, J., Bentley, W. E., Rao, G. 1997. On-line green fluorescent protein sensor with LED excitation. Biotech. Bioeng: to appear.
40. Reed, L., Muench, H. 1938. A simple method for estimating fifty percent endpoints. Am. J. Hyg. 27: 493-497.
41. Reilander, H., Haase, W., Maul, G. 1996. Functional expression of the Aequorea victoria green fluorescent protein in insect cells using the baculovirus expression system. Biochem. Biophys. Res. Commun. 219: 14-20.
42. Rosenburg, S. A., Grimm, E. A., McGrogan, M., Doyle, M., Kawasaki, E., Koghs, K., Mark, D. F. 1984. Lymophokine activated T cells in the treatment of cancer. Science 223: 1412-1415.
43. + RNA in the cytoplasm. Proc. Natl. Acad. Sci. USA 92: 225-229.
44. Shimomura, O., Johnson, F. H., Saiga, Y. 1962. Excitation, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan. Aequorea. J. Cell. Comp. Physiol. 59: 223-227.
45. Slack, J. M., Kuzio, J., Faulkner, P. 1995. Characterization of v-cath, a cathepsin L-like proteinase expressed by the baculovirus Autographa californica nuclear polyhedrosis virus. J Gen. Virol. 76: 1091-1098.
46. Stearns, T. 1995. The green revolution. Current Bio. 5: 262-264.
47. Summers, M. D., Smith, G. E. 1987. A manual of methods for baculovirus vectors and insect cell culture procedure. Texas Agricultural Experiment Station Bulletin. No. 1555.
48. 2 expressed in Trichoplusia ni larvae - Isolation and kinetic properties of the enzyme. Protein Expression Purif. 4: 490-498.
49. Vaughn, J. L., Goodwin, R. H., Tompkins, G. J., McCawley, P. 1977. The establishment of two insect cell lines from the insect Spodoptera frugiperda (Lepidoptera, Noctuidae). In Vitro 13: 213-217.
50. Wang, M. Y., Pulliam, T. R., Valle, M., Vakharia, V., Bentley, W. E. 1996. Kinetic analysis of alkaline protease activity, recombinant protein production and metabolites for infected insect (Sf9) cells under different DO levels. J. Biotechnol. 46: 243-254.
51. Wu, G.-I., Zou, D.-J., Koothan, T., Cline, H. T. 1995. Infection of frog neurons with vaccinia virus permits in vivo expression of foreign proteins. Neuron 14: 681-684.