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Volumn 10, Issue 2, 1999, Pages 142-145

Modifying secretion and post-translational processing in insect cells

Author keywords

[No Author keywords available]

Indexed keywords

CHAPERONE; MEMBRANE PROTEIN; OLIGOSACCHARIDE; PROTEIN; SIGNAL PEPTIDE;

EID: 0033022809     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-1669(99)80024-X     Document Type: Article
Times cited : (91)

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    • This paper demonstrates that a limitation in insect cell proprotein processing can be overcome by coexpressing a gene for a mammalian proprotein convertase. A transforming growth factor was efficiently processed from its proprotein form into its mature form when produced in the presence of a recombinant furin. The activity of the TGF was increased by a factor of 7.8 when coproduced with furin.
    • Laprise M-H., Grondin F., Dubois C.M. Enhanced TGFβ1 maturation in High Five cells coinfected with recombinant baculovirus encoding the convertase furin/pace: improved technology for the production of recombinant proproteins in insect cells. Biotechnol Bioeng. 58:1998;85-91. This paper demonstrates that a limitation in insect cell proprotein processing can be overcome by coexpressing a gene for a mammalian proprotein convertase. A transforming growth factor was efficiently processed from its proprotein form into its mature form when produced in the presence of a recombinant furin. The activity of the TGF was increased by a factor of 7.8 when coproduced with furin.
    • (1998) Biotechnol Bioeng , vol.58 , pp. 85-91
    • Laprise, M.-H.1    Grondin, F.2    Dubois, C.M.3


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