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Seidah, N.G.1
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Dong, W.4
Tardos, H.5
Mbikay, M.6
Chretien, M.7
Day, R.8
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44
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0032485534
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Enhanced TGFβ1 maturation in High Five cells coinfected with recombinant baculovirus encoding the convertase furin/pace: Improved technology for the production of recombinant proproteins in insect cells
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This paper demonstrates that a limitation in insect cell proprotein processing can be overcome by coexpressing a gene for a mammalian proprotein convertase. A transforming growth factor was efficiently processed from its proprotein form into its mature form when produced in the presence of a recombinant furin. The activity of the TGF was increased by a factor of 7.8 when coproduced with furin.
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Laprise M-H., Grondin F., Dubois C.M. Enhanced TGFβ1 maturation in High Five cells coinfected with recombinant baculovirus encoding the convertase furin/pace: improved technology for the production of recombinant proproteins in insect cells. Biotechnol Bioeng. 58:1998;85-91. This paper demonstrates that a limitation in insect cell proprotein processing can be overcome by coexpressing a gene for a mammalian proprotein convertase. A transforming growth factor was efficiently processed from its proprotein form into its mature form when produced in the presence of a recombinant furin. The activity of the TGF was increased by a factor of 7.8 when coproduced with furin.
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(1998)
Biotechnol Bioeng
, vol.58
, pp. 85-91
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Laprise, M.-H.1
Grondin, F.2
Dubois, C.M.3
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