메뉴 건너뛰기




Volumn 21, Issue 12, 2003, Pages 1473-1479

Fluorobodies combine GFP fluorescence with the binding characteristics of antibodies

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; DRUG PRODUCTS; ENGINEERING RESEARCH; FLUORESCENCE; GENES;

EID: 0346031536     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt911     Document Type: Article
Times cited : (29)

References (41)
  • 1
    • 0026233070 scopus 로고
    • Surface presentation of protein epitopes using bacteriophage expression systems
    • Smith, G.P. Surface presentation of protein epitopes using bacteriophage expression systems. Curr. Opin. Biotechnol. 2, 668-673 (1991).
    • (1991) Curr. Opin. Biotechnol. , vol.2 , pp. 668-673
    • Smith, G.P.1
  • 2
    • 0025112794 scopus 로고
    • Searching for peptide ligands with an epitope library
    • Scott, J.K. & Smith, G.P. Searching for peptide ligands with an epitope library. Science 249, 386-390 (1990).
    • (1990) Science , vol.249 , pp. 386-390
    • Scott, J.K.1    Smith, G.P.2
  • 3
    • 0033987925 scopus 로고    scopus 로고
    • Exploiting recombination in single bacteria to make large phage antibody libraries
    • Sblattero, D. & Bradbury, A. Exploiting recombination in single bacteria to make large phage antibody libraries. Nat. Biotechnol. 18, 75-80 (2000).
    • (2000) Nat. Biotechnol. , vol.18 , pp. 75-80
    • Sblattero, D.1    Bradbury, A.2
  • 4
    • 0026317443 scopus 로고
    • By-passing immunization. Human antibodies from V-gene libraries displayed on phage
    • Marks, J.D. et al. By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222, 581-597 (1991).
    • (1991) J. Mol. Biol. , vol.222 , pp. 581-597
    • Marks, J.D.1
  • 5
    • 9344223986 scopus 로고    scopus 로고
    • Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library
    • Vaughan, T.J. et al. Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nat. Biotechnol. 14, 309-314 (1996).
    • (1996) Nat. Biotechnol. , vol.14 , pp. 309-314
    • Vaughan, T.J.1
  • 6
    • 0033865190 scopus 로고    scopus 로고
    • Engineered protein scaffolds for molecular recognition
    • Skerra, A. Engineered protein scaffolds for molecular recognition. J. Mol. Recognit. 13, 167-187 (2000).
    • (2000) J. Mol. Recognit. , vol.13 , pp. 167-187
    • Skerra, A.1
  • 7
    • 0031663369 scopus 로고    scopus 로고
    • The green fluorescent protein
    • Tsien, R.Y. The green fluorescent protein. Annu. Rev. Biochem. 67, 509-544 (1998).
    • (1998) Annu. Rev. Biochem. , vol.67 , pp. 509-544
    • Tsien, R.Y.1
  • 8
    • 0035793148 scopus 로고    scopus 로고
    • Display of green fluorescent protein on Escherichia coli cell surface
    • Shi, H. & Wen Su, W. Display of green fluorescent protein on Escherichia coli cell surface. Enzyme Microb. Technol. 28, 25-34 (2001).
    • (2001) Enzyme Microb. Technol. , vol.28 , pp. 25-34
    • Shi, H.1    Wen Su, W.2
  • 9
    • 0032518204 scopus 로고    scopus 로고
    • Green fluorescent protein as a scaffold for intracellular presentation of peptides
    • Abedi, M.R., Caponigro, G. & Kamb, A. Green fluorescent protein as a scaffold for intracellular presentation of peptides. Nucleic Acids Res. 26, 623-630 (1998).
    • (1998) Nucleic Acids Res. , vol.26 , pp. 623-630
    • Abedi, M.R.1    Caponigro, G.2    Kamb, A.3
  • 10
    • 0035019773 scopus 로고    scopus 로고
    • Intracellular protein scaffold-mediated display of random peptide libraries for phenotypic screens in mammalian cells
    • Peelle, B. et al. Intracellular protein scaffold-mediated display of random peptide libraries for phenotypic screens in mammalian cells. Chem. Biol. 8, 521-534 (2001).
    • (2001) Chem. Biol. , vol.8 , pp. 521-534
    • Peelle, B.1
  • 11
    • 0030834492 scopus 로고    scopus 로고
    • A genetically encoded optical probe of membrane voltage
    • Siegel, M.S. & Isacoff, E.Y. A genetically encoded optical probe of membrane voltage. Neuron 19, 735-741 (1997).
    • (1997) Neuron , vol.19 , pp. 735-741
    • Siegel, M.S.1    Isacoff, E.Y.2
  • 12
    • 0032784511 scopus 로고    scopus 로고
    • Design of generic biosensors based on green fluorescent proteins with allosteric sites by directed evolution
    • Doi, N. & Yanagawa, H. Design of generic biosensors based on green fluorescent proteins with allosteric sites by directed evolution. FEBS Lett. 453, 305-307 (1999).
    • (1999) FEBS Lett. , vol.453 , pp. 305-307
    • Doi, N.1    Yanagawa, H.2
  • 13
    • 0032500053 scopus 로고    scopus 로고
    • Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins
    • Miesenbock, G., De Angelis, D.A. & Rothman, J.E. Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394, 192-195 (1998).
    • (1998) Nature , vol.394 , pp. 192-195
    • Miesenbock, G.1    De Angelis, D.A.2    Rothman, J.E.3
  • 14
    • 0033613235 scopus 로고    scopus 로고
    • Circular permutation and receptor insertion within green fluorescent proteins
    • Baird, G.S., Zacharias, D.A. & Tsien, R.Y. Circular permutation and receptor insertion within green fluorescent proteins. Proc. Natl. Acad. Sci. USA 96, 11241-11246 (1999).
    • (1999) Proc. Natl. Acad. Sci. USA , vol.96 , pp. 11241-11246
    • Baird, G.S.1    Zacharias, D.A.2    Tsien, R.Y.3
  • 15
    • 0021818675 scopus 로고
    • Filamentous fusion phage: Novel expression vectors that display cloned antigens on the virion surface
    • Smith, G.P. Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science 228, 1315-1317 (1985).
    • (1985) Science , vol.228 , pp. 1315-1317
    • Smith, G.P.1
  • 16
    • 0025801451 scopus 로고
    • Immunoglobulin gene 'fingerprinting': An approach to analysis of B lymphoid clonality in lymphoproliferative disorders
    • Deane, M. & Norton, J.D. Immunoglobulin gene 'fingerprinting': an approach to analysis of B lymphoid clonality in lymphoproliferative disorders. Br. J. Haematol. 77, 274-281 (1991).
    • (1991) Br. J. Haematol. , vol.77 , pp. 274-281
    • Deane, M.1    Norton, J.D.2
  • 17
    • 0035400341 scopus 로고    scopus 로고
    • Antibodies in haystacks: How selection strategy influences the outcome of selection from molecular diversity libraries
    • Lou, J. et al. Antibodies in haystacks: how selection strategy influences the outcome of selection from molecular diversity libraries. J. Immunol. Methods 253, 233-242 (2001).
    • (2001) J. Immunol. Methods , vol.253 , pp. 233-242
    • Lou, J.1
  • 18
    • 0036591970 scopus 로고    scopus 로고
    • Neuronal calcium sensor-1 binds to regulated secretory organelles and functions in basal and stimulated exocytosis in PC12 cells
    • Scalettar, B.A. et al. Neuronal calcium sensor-1 binds to regulated secretory organelles and functions in basal and stimulated exocytosis in PC12 cells. J. Cell Sci. 115, 2399-2412 (2002).
    • (2002) J. Cell Sci. , vol.115 , pp. 2399-2412
    • Scalettar, B.A.1
  • 19
    • 0025876152 scopus 로고
    • Kinetic analysis of monoclonal antibody-antigen interactions with a new biosensor based analytical system
    • Karlsson, R., Michaelsson, A. & Mattsson, L. Kinetic analysis of monoclonal antibody-antigen interactions with a new biosensor based analytical system. J. Immunol. Methods 145, 229-240 (1991).
    • (1991) J. Immunol. Methods , vol.145 , pp. 229-240
    • Karlsson, R.1    Michaelsson, A.2    Mattsson, L.3
  • 20
    • 0000637270 scopus 로고    scopus 로고
    • Flow cytometry: A versatile tool for all phases of drug discovery
    • Nolan, J.P., Lauer, S., Prossnitz, E.R. & Sklar, L.A. Flow cytometry: a versatile tool for all phases of drug discovery. Drug Discov. Today 4, 173-180 (1999).
    • (1999) Drug Discov. Today , vol.4 , pp. 173-180
    • Nolan, J.P.1    Lauer, S.2    Prossnitz, E.R.3    Sklar, L.A.4
  • 21
    • 0032479180 scopus 로고    scopus 로고
    • Expression of an antibody fragment at high levels in the bacterial cytoplasm
    • Martineau, P., Jones, P. & Winter, G. Expression of an antibody fragment at high levels in the bacterial cytoplasm. J. Mol. Biol. 280, 117-127 (1998).
    • (1998) J. Mol. Biol. , vol.280 , pp. 117-127
    • Martineau, P.1    Jones, P.2    Winter, G.3
  • 22
    • 0034625458 scopus 로고    scopus 로고
    • Structural dynamics of green fluorescent protein alone and fused with a single chain Fv protein
    • Hink, M.A. et al. Structural dynamics of green fluorescent protein alone and fused with a single chain Fv protein. J. Biol. Chem. 275, 17556-17560 (2000).
    • (2000) J. Biol. Chem. , vol.275 , pp. 17556-17560
    • Hink, M.A.1
  • 24
    • 0028291823 scopus 로고
    • Isolation of high affinity human antibodies directly from large synthetic repertoires
    • Griffiths, A.D. et al. Isolation of high affinity human antibodies directly from large synthetic repertoires. EMBO J. 13, 3245-3260 (1994).
    • (1994) EMBO J. , vol.13 , pp. 3245-3260
    • Griffiths, A.D.1
  • 25
    • 0034705696 scopus 로고    scopus 로고
    • Development and application of cytotoxic T lymphocyte-associated antigen 4 as a protein scaffold for the generation of novel binding ligands
    • Hufton, S.E. et al. Development and application of cytotoxic T lymphocyte-associated antigen 4 as a protein scaffold for the generation of novel binding ligands. FEBS Lett. 475, 225-231 (2000).
    • (2000) FEBS Lett. , vol.475 , pp. 225-231
    • Hufton, S.E.1
  • 26
    • 0035854524 scopus 로고    scopus 로고
    • Building novel binding ligands to B7.1 and B7.2 based on human antibody single variable light chain domains
    • van den Beucken, T. et al. Building novel binding ligands to B7.1 and B7.2 based on human antibody single variable light chain domains. J. Mol. Biol. 310, 591-601 (2001).
    • (2001) J. Mol. Biol. , vol.310 , pp. 591-601
    • Van Den Beucken, T.1
  • 27
    • 0029746203 scopus 로고    scopus 로고
    • Single antibody domains as small recognition units: Design and in vitro antigen selection of camelized, human VH domains with improved protein stability
    • Davies, J. & Riechmann, L. Single antibody domains as small recognition units: design and in vitro antigen selection of camelized, human VH domains with improved protein stability. Protein Eng. 9, 531-537 (1996).
    • (1996) Protein Eng. , vol.9 , pp. 531-537
    • Davies, J.1    Riechmann, L.2
  • 28
    • 0035715878 scopus 로고    scopus 로고
    • In vivo recombination as a tool to generate molecular diversity in phage antibody libraries
    • Sblattero, D., Lou, J., Marzari, R. & Bradbury, A. In vivo recombination as a tool to generate molecular diversity in phage antibody libraries. J. Biotechnol. 74, 303-315 (2001).
    • (2001) J. Biotechnol. , vol.74 , pp. 303-315
    • Sblattero, D.1    Lou, J.2    Marzari, R.3    Bradbury, A.4
  • 29
    • 13144261717 scopus 로고    scopus 로고
    • Efficient construction of a large nonimmune phage antibody library; the production of panels of high affinity human single-chain antibodies to protein antigens
    • Sheets, M.D. et al. Efficient construction of a large nonimmune phage antibody library; the production of panels of high affinity human single-chain antibodies to protein antigens. Proc. Natl. Acad. Sci. USA 95, 6157-6162 (1998).
    • (1998) Proc. Natl. Acad. Sci. USA , vol.95 , pp. 6157-6162
    • Sheets, M.D.1
  • 31
    • 0032510783 scopus 로고    scopus 로고
    • A bacterial two-hybrid system based on a reconstituted signal transduction pathway
    • Karimova, G., Pidoux, J., Ullmann, A. & Ladant, D. A bacterial two-hybrid system based on a reconstituted signal transduction pathway. Proc. Natl. Acad. Sci. USA 95, 5752-5756 (1998).
    • (1998) Proc. Natl. Acad. Sci. USA , vol.95 , pp. 5752-5756
    • Karimova, G.1    Pidoux, J.2    Ullmann, A.3    Ladant, D.4
  • 32
    • 0034665746 scopus 로고    scopus 로고
    • Mass spectral analysis of a protein complex using single-chain antibodies selected on a peptide target: Applications to functional genomics
    • Siegel, R.W., Allen, B., Pavlik, P., Marks, J.D. & Bradbury, A. Mass spectral analysis of a protein complex using single-chain antibodies selected on a peptide target: applications to functional genomics. J. Mol. Biol. 302, 285-293 (2000).
    • (2000) J. Mol. Biol. , vol.302 , pp. 285-293
    • Siegel, R.W.1    Allen, B.2    Pavlik, P.3    Marks, J.D.4    Bradbury, A.5
  • 33
    • 0036299049 scopus 로고    scopus 로고
    • The intracellular antibody capture technology (IACT): Towards a consensus sequence for intracellular antibodies
    • Visintin, M. et al. The intracellular antibody capture technology (IACT): towards a consensus sequence for intracellular antibodies. J. Mol. Biol. 317, 73-83 (2002).
    • (2002) J. Mol. Biol. , vol.317 , pp. 73-83
    • Visintin, M.1
  • 34
    • 0035942736 scopus 로고    scopus 로고
    • Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
    • Elbashir, S.M. et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494-498 (2001).
    • (2001) Nature , vol.411 , pp. 494-498
    • Elbashir, S.M.1
  • 35
    • 0037685280 scopus 로고    scopus 로고
    • Expression profiling reveals off-target gene regulation by RNAi
    • Jackson, A.L. et al. Expression profiling reveals off-target gene regulation by RNAi. Nat. Biotechnol. 21, 635-637 (2003).
    • (2003) Nat. Biotechnol. , vol.21 , pp. 635-637
    • Jackson, A.L.1
  • 36
    • 0038419800 scopus 로고    scopus 로고
    • Induction of an interferon response by RNAi vectors in mammalian cells
    • Bridge, A.J., Pebernard, S., Ducraux, A., Nicoulaz, A.L. & Iggo, R. Induction of an interferon response by RNAi vectors in mammalian cells. Nat. Genet. 34, 263-264 (2003).
    • (2003) Nat. Genet. , vol.34 , pp. 263-264
    • Bridge, A.J.1    Pebernard, S.2    Ducraux, A.3    Nicoulaz, A.L.4    Iggo, R.5
  • 38
    • 0031829517 scopus 로고    scopus 로고
    • The emergence of flow cytometry for sensitive, real-time measurements of molecular interactions
    • Nolan, J.P. & Sklar, L.A. The emergence of flow cytometry for sensitive, real-time measurements of molecular interactions. Nat. Biotechnol. 16, 633-638 (1998).
    • (1998) Nat. Biotechnol. , vol.16 , pp. 633-638
    • Nolan, J.P.1    Sklar, L.A.2
  • 39
    • 0031697577 scopus 로고    scopus 로고
    • Real-time imaging of the axonal transport of granules containing a tissue plasminogen activator/green fluorescent protein hybrid
    • Lochner, J.E. etal. Real-time imaging of the axonal transport of granules containing a tissue plasminogen activator/green fluorescent protein hybrid. Mol. Biol. Cell 9, 2463-2476 (1998).
    • (1998) Mol. Biol. Cell , vol.9 , pp. 2463-2476
    • Lochner, J.E.1
  • 40
    • 0004285740 scopus 로고    scopus 로고
    • (ed. Goslin, G.B.a.K.) (MIT Press, Cambridge, MA, USA
    • Goslin, K. & Banker, G. in Culturing Nerve Cells (ed. Goslin, G.B.a.K.) 252-281 (MIT Press, Cambridge, MA, USA, 1997).
    • (1997) Culturing Nerve Cells , pp. 252-281
    • Goslin, K.1    Banker, G.2
  • 41
    • 0002378571 scopus 로고    scopus 로고
    • Dispersion, aberration and deconvolution in multi-wavelength fluorescence images
    • Scalettar, B.A., Swedlow, J.R., Sedat, J.W. & Agard, D.A. Dispersion, aberration and deconvolution in multi-wavelength fluorescence images. J. Microsc. 182 (Pt 1), 50-60 (1996).
    • (1996) J. Microsc. , vol.182 , Issue.PART 1 , pp. 50-60
    • Scalettar, B.A.1    Swedlow, J.R.2    Sedat, J.W.3    Agard, D.A.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.