Mass spectral analysis of a protein complex using single-chain antibodies selected on a peptide target: Applications to functional genomics

Journal of Molecular Biology

Volumn 302, Issue 2, 2000, Pages 285-293

  Siegel, Robert W ^a   Allen, Beth ^a   Pavlik, Peter ^a   Marks, James D ^b   Bradbury, Andrew ^a,c  

^a LOS ALAMOS NATIONAL LABORATORY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c SISSA   (Italy)

Author keywords

Functional genomics; Mass spectrometry; Peptide selection; Phage display; Single chain variable fragment

Indexed keywords

ANTIBODY; DOUBLE STRANDED DNA; KU ANTIGEN; PEPTIDE; PROTEIN;

AMINO ACID SEQUENCE; ANTIBODY AFFINITY; ANTIGEN ANTIBODY REACTION; ARTICLE; CHROMOSOME BREAKAGE; CONTROLLED STUDY; DNA LIBRARY; GENE EXPRESSION; GENE FUNCTION; GENE LOCATION; HUMAN; IMMUNOBLOTTING; IMMUNOPRECIPITATION; MASS SPECTROMETRY; PHAGE DISPLAY; PRIORITY JOURNAL;

EID: 0034665746     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4070     Document Type: Article

References (28)

1. A central region of Ku80 mediates interaction with Ku70 in vivo
2. Disruption of anthrax toxin binding with the use of human antibodies and competitive inhibitors
3. Exploring the metabolic and genetic control of gene expression on a genomic scale
4. Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids
5. Human anti-self antibodies with high specificity from phage display libraries
6. Selection of phage antibodies by binding affinity: Mimicking affinity maturation
7. Protein surface analysis. Methods for identifying antigenic determinants and other interaction sites
8. A computer program for predicting protein antigenic determinants
9. Diverting a protein from its cellular location by intracellular antibodies: The case of p21Ras
10. By-passing immunization. Human antibodies from V-gene libraries displayed on phage
11. A biochemical genomics approach for identifying genes by the activity of their products
12. Phage antibodies: Filamentous phage displaying antibody variable domains
13. Mass spectrometry and EST-database searching allows characterization of the multiprotein spliceosome complex
14. Correlation between the location of antigenic sites and the prediction of turns in proteins
15. Single-chain variable fragments selected on the 57-76 p21Ras neutralising epitope from phage antibody libraries recognise the native protein
16. Antibodies against a preselected peptide recognize and neutralize foot and mouth disease virus
17. Large-scale analysis of the yeast genome by transposon tagging and gene disruption
18. Exploiting recombination in single bacteria to make large phage antibody libraries
19. Production of fluorescent single-chain antibody fragments in Escherichia coil
20. Efficient construction of a large non-immune phage antibody library; the production of panels of high-affinity human single-chain antibodies to protein antigens
21. Linking genome and proteome by mass spectrometry: Large-scale identification of yeast proteins from two dimensional gels
22. Chemical synthesis of a polypeptide predicted from nucleotide sequence allows detection of a new retroviral gene product
23. The reactivity of anti-peptide antibodies is a function of the atomic mobility of sites in a protein
24. Location of 'continuous' antigenic determinants in the protruding regions of proteins
25. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae
26. Antibodies specific for the carboxy and amino-terminal regions of simian virus 40 large tumor antigen
27. Human single-chain Fv immunoconjugates targeted to a melanoma-associated chondroitin sulfate proteoglycan mediate specific lysis of human melanoma cells by natural killer cells and complement
28. Extended half-life and elevated steady-state level of a single-chain Fv intrabody are critical for specific intracellular retargeting of its antigen, caspase-7