Hsp90 binds CpG oligonucleotides directly: Implications for Hsp90 as a missing link in CpG signaling and recognition

Cellular and Molecular Life Sciences

Volumn 60, Issue 2, 2003, Pages 422-429

  Bandholtz, L ^a   Guo, Y ^b   Palmberg, C ^c   Mattsson, K ^a   Ohlsson, B ^a   High, A ^b   Shabanowitz, J ^b   Hunt, D F ^b   Jornvall H ^c   Wigzell, H ^a   Agerberth, B ^c   Gudmundsson, G H ^a,d  

^a KAROLINSKA INSTITUTE   (Sweden)

^b UNIVERSITY OF VIRGINIA   (United States)

^c KAROLINSKA INSTITUTE   (Sweden)

^d Institute of Biology   (Iceland)

Author keywords

CpG DNA; CpG signaling; Innate immunity; Pattern recognition; TLR 9

Indexed keywords

BACTERIAL DNA; CELL EXTRACT; HEAT SHOCK PROTEIN 90; LIGAND; OLIGODEOXYNUCLEOTIDE; OLIGONUCLEOTIDE; TOLL LIKE RECEPTOR; TOLL LIKE RECEPTOR 9;

ARTICLE; CONTROLLED STUDY; CPG ISLAND; GEL ELECTROPHORESIS; GEL MOBILITY SHIFT ASSAY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; IMMUNE SYSTEM; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOHISTOCHEMISTRY; MOLECULAR RECOGNITION; MONONUCLEAR CELL; PROTEIN ANALYSIS; PROTEIN DNA BINDING; PROTEIN FUNCTION; PROTEIN ISOLATION; PROTEIN LOCALIZATION; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; REVERSED PHASE LIQUID CHROMATOGRAPHY; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION;

CELL LINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA-BINDING PROTEINS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ELECTROPHORETIC MOBILITY SHIFT ASSAY; HSP90 HEAT-SHOCK PROTEINS; HUMANS; JURKAT CELLS; LEUKOCYTES, MONONUCLEAR; NANOTECHNOLOGY; OLIGODEOXYRIBONUCLEOTIDES; RECEPTORS, CELL SURFACE; SIGNAL TRANSDUCTION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TOLL-LIKE RECEPTOR 9; U937 CELLS;

BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 0345268793     PISSN: 1420682X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s000180300035     Document Type: Article

References (30)

1. Krieg A. M. (2002) CpG motifs in bacterial DNA and their immune effects. Annu. Rev. Immunol. 20: 709-760
2. Krieg A. M., Yi A. K., Matson S., Waldschmidt T. J., Bishop G. A., Teasdale R. et al. (1995) CpG motifs in bacterial DNA trigger direct B-cell activation. Nature 374: 546-549
3. Hemmi H., Takeuchi O., Kawai T., Kaisho T., Sato S., Sanjo H. et al. (2000) A Toll-like receptor recognizes bacterial DNA. Nature 408: 740-745
4. Lien E. and Ingalls R. R. (2002) Toll-like receptors. Crit. Care Med. 30: S1-S11
5. Medzhitov R. and Janeway C. Jr (2000) The Toll receptor family and microbial recognition. Trends Microbiol. 8: 452-456
6. Vabulas R. M., Ahmad-Nejad P., Costa C. da, Miethke T., Kirschning C. J., Hacker H. et al. (2001) Endocytosed HSP60s use toll-like receptor 2 (TLR2) and TLR4 to activate the toll/interleukin-1 receptor signaling pathway in innate immune cells. J. Biol. Chem. 276: 31332-31339
7. Vabulas R. M., Braedel S., Hilf N., Singh-Jasuja H., Herter S., Ahmad-Nejad P. et al. (2002) The ER-resident heat shock protein Gp96 activates dendritic cells via the TLR2/4 pathway. J. Biol. Chem. 23: 23
8. Vabulas R. M., Ahmad-Nejad P., Ghose S., Kirschning C. J., Issels R. D. and Wagner H. (2002) HSP70 as endogenous stimulus of the Toll/interleukin-1 receptor signal pathway. J. Biol. Chem. 277: 15107-15112
9. Ausubel F. M., Brent R., Kingstone R. E., Moore D. D., Seidman J. G., Smith J. A. et al. (1987) Current Protocols in Molecular Biology, Wiley, New York
10. Shevchenko A., Wilm M., Vorm O. and Mann M. (1996) Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal. Chem. 68: 850-858
11. Ficarro S. B., McCleland M. L., Stukenberg P. T., Burke D. J., Ross M. M., Shabanowitz J. et al. (2002) Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae. Nat. Biotechnol. 20: 301-305
12. Martin S. E., Shabanowitz J., Hunt D. F. and Marto J. A. (2000) Subfemtomole MS and MS/MS peptide sequence analysis using nano-HPLC micro-ESI fourier transform ion cyclotron resonance mass spectrometry. Anal. Chem. 72: 4266-4274
13. Yamamoto T., Yamamoto S., Kataoka T. and Tokunaga T. (1994) Lipofection of synthetic oligodeoxyribonucleotide having a palindromic sequence of AACGTT to murine splenocytes enhances interferon production and natural killer activity. Microbiol. Immunol. 38: 831-836
14. Yamamoto T., Yamamoto S., Kataoka T., Komuro K., Kohase M. and Tokunaga T. (1994) Synthetic oligonucleotides with certain palindromes stimulate interferon production of human peripheral blood lymphocytes in vitro. Jpn. J. Cancer Res. 85: 775-779
15. Hartmann G. and Krieg A. M. (2000) Mechanism and function of a newly identified CpG DNA motif in human primary B cells. J. Immunol. 164: 944-953
16. Janeway C. A. Jr and Medzhitov R. (2002) Innate immune recognition. Annu. Rev. Immunol. 20: 197-216
17. Hacker G., Redecke V. and Hacker H. (2002) Activation of the immune system by bacterial CpG-DNA. Immunology 105: 245-251
18. Beutler B. (2002) Toll-like receptors: how they work and what they do. Curr. Opin. Hematol. 9: 2-10
19. Medzhitov R. (2001) Toll-like receptors and innate immunity. Nat. Rev. Immunol. 1: 135-145
20. Srivastava P. K., Menoret A., Basu S., Binder R. J. and McQuade K. L. (1998) Heat shock proteins come of age: primitive functions acquire new roles in an adaptive world. Immunity 8: 657-665
21. Ueki K., Tabeta K., Yoshie H. and Yamazaki K. (2002) Self-heat shock protein 60 induces tumour necrosis factor-alpha in monocyte-derived macrophage: possible role in chronic inflammatory periodontal disease. Clin. Exp. Immunol. 127: 72-77
22. Takeshita F., Leifer C. A., Gursel I., Ishii K. J., Takeshita S., Gursel M. et al. (2001) Cutting edge: role of Toll-like receptor 9 in CpG DNA-induced activation of human cells. J. Immunol. 167: 3555-3558
23. Triantafilou K., Triantafilou M., Ladha S., Mackie A., Fernandez N., Dedrick R. L. et al. (2001) Fluorescence recovery after photobleaching reveals that LPS rapidly transfers from CD14 to hsp70 and hsp90 on the cell membrane. J. Cell. Sci. 114: 2535-2545
24. Triantafilou K., Triantafilou M. and Dedrick R. L. (2001) A CD14-independent LPS receptor cluster. Nat. Immunol. 2: 338-345
25. Zhu F. G. and Pisetsky D. S. (2001) Role of the heat shock protein 90 in immune response stimulation by bacterial DNA and synthetic oligonucleotides. Infect. Immun. 69: 5546-5552
26. Chu W., Gong X., Li Z., Takabayashi K., Ouyang H., Chen Y. et al. (2000) DNA-PKcs is required for activation of innate immunity by immunostimulatory DNA. Cell 103: 909-918
27. Karpova A. Y., Trost M., Murray J. M., Cantley L. C. and Howley P. M. (2002) Interferon regulatory factor-3 is an in vivo target of DNA-PK. Proc. Natl. Acad. Sci. USA 99: 2818-2823
28. Freeman B. C. and Yamamoto K. R. (2002) Disassembly of transcriptional regulatory complexes by molecular chaperones. Science 296: 2232-2235
29. Janeway C. A. Jr (1989) Approaching the asymptote? Evolution and revolution in immunology. Cold Spring Harb. Symp. Quant. Biol. 54: 1-13
30. Matzinger P. (1998) An innate sense of danger. Semin. Immunol. 10: 399-415