Fluorescence recovery after photobleaching reveals that LPS rapidly transfers from CD14 to hsp70 and hsp90 on the cell membrane

Journal of Cell Science

Volumn 114, Issue 13, 2001, Pages 2535-2545

  Triantafilou, K ^a   Triantafilou, M ^a   Ladha, S ^a   Mackie, A ^a   Fernandez, N ^a   Dedrick, R L ^a   Cherry, R ^a  

^a UNIVERSITY OF PORTSMOUTH   (United Kingdom)

Author keywords

CD14; Endotoxin; FRAP; Lateral diffusion; Lipopolysaccharide

Indexed keywords

ANTIBODY; BACTERIUM LIPOPOLYSACCHARIDE; CD14 ANTIGEN; CELL RECEPTOR; FLUORESCEIN ISOTHIOCYANATE; HEAT SHOCK PROTEIN 70; HEAT SHOCK PROTEIN 90; INTERLEUKIN 6; LIPOTEICHOIC ACID; PEPTIDOGLYCAN;

ANIMAL CELL; ANTIGEN BINDING; ANTIGEN RECOGNITION; ARTICLE; BLEACHING; CELL MEMBRANE; CELL WALL; COMPLEX FORMATION; CONTROLLED STUDY; CYTOKINE PRODUCTION; DIFFUSION; DYNAMICS; FEMALE; GRAM NEGATIVE BACTERIUM; IMMOBILIZATION; IMMUNOSTIMULATION; NONHUMAN; PRIORITY JOURNAL; STIMULATION;

ANIMALS; ANTIGENS, CD14; CELL MEMBRANE; CHO CELLS; CRICETINAE; FLUORESCENCE; HSP70 HEAT-SHOCK PROTEINS; HSP90 HEAT-SHOCK PROTEINS; LIPOPOLYSACCHARIDES; PHOTOCHEMISTRY; TIME FACTORS;

ANIMALIA; BACTERIA (MICROORGANISMS); NEGIBACTERIA; POSIBACTERIA;

EID: 0034928401     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (38)

1. Lipopolysaccharide complexed with soluble CD14 binds to normal human monocytes
2. The pathogenesis of sepsis
3. A method to detect 2-keto-3-deoxyoctanat and related compounds on pherograms and chromatograms
4. Heat shock 90 mediates macrophage activation by Taxol and bacterial lipopolysaccharide
5. Response of neutrophils to LPS. Different signalling pathways for CD18 mediated adhesion and Fc-mediated phagocytosis
6. The 2 soluble forms of the lipopolysaccharide receptor, CD14 - Characterization and release by normal human monocytes
7. Soluble CD14 participates in the response of cells to lipopolysaccharide
8. Lipopolysaccharide (LPS) Signal-transduction and clearance - Dual roles for LPS binding-protein and membrane CD14
9. The immunological synapse: A molecular machine controlling T cell activation
10. CD14 is a cell-activating receptor for bacterial peptidoglycan
11. GPI-microdomains: A role in signalling via immunoreceptors
12. CD11c/CD18, a transmembrane signaling receptor for lipopolysaccharide
13. Outside-in signalling by lipopolysaccharide through a tailless integrin
14. Human Toll-like receptor 2 confers responsiveness to bacterial lipopolyssacharide
15. Release from a human monocyte-like cell line of two different soluble forms of the lipopolysaccharide receptor, CD14
16. Check cell membrane fluidity measured by fluorescence recovery after photobleaching and steady state anisotropy
17. Lateral diffusion in planar lipid bilayers. A FRAP investigation of its modulation by lipid composition, cholesterol or alamethicin content and divalent cations
18. Glycosyl-phosphatidylinositol-anchored or integral membrane forms of CD14 mediate identical cellular-responses to endotoxin
19. Neither CD14 nor serum is absolutely necessary for activation of mononuclear phagocytes by bacterial lipopolysaccharide
20. Three-dimensional segregation of supramolecular activation clusters in T cells
21. Defective LPS signaling in C3H/Hej and C57BL/10ScCr mice: Mutations in TLR4 gene
22. Lipopolysaccharide activation of human endothelial and epithelial cells is mediated by lipopolysaccharide-binding protein and soluble CD14
23. CD14 is a pattern-recognition receptor
24. Structure and function of lipopolysaccharide binding protein
25. Porphyronomas gingivalis lipopolysaccharide stimulation of human monocytes: Dependence on serum and CD14 receptor
26. Imaging of individual cell surface MHC molecules using fluorescent particles
27. An amino-terminal fragment of human lipopolysaccharide-binding protein retains lipid A binding but not CD14-stimulatory activity
28. Isolation of a lipopolysaccharide-binding acute phase reactant from rabbit serum
29. Moesin functions as a lipopolysaccharide receptor on human monocytes
30. Lipopolysaccharide labelled with Alexa 488 Hydrazide as a novel probe for LPS binding studies
31. Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
32. Rough and smooth forms of fluorescein-labelled bacterial endotoxin exhibit CD14/LBP dependent and independent binding that is influenced by endotoxin concentration
33. Saturable CD14-dependent binding of fluorescein-labelled lipopolysaccharide to human monocytes
34. A region of human CD14 required for lipopolysaccharide-binding
35. Soluble peptidoglycan-induced monokine production can be blocked by anti-CD14 monoclonal-antibodies and by lipid-A partial structures
36. The effect of bleaching light on measurements of lateral diffusion in cell membranes by the fluorescence photobleaching recovery method
37. Lipopolysaccharide (LPS) binding protein opsonises LPS-bearing particles for recognition by a novel receptor on macrophages
38. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding-protein