Gα12 regulates epithelial cell junctions through Src tyrosine kinases

American Journal of Physiology - Cell Physiology

Volumn 285, Issue 5 54-5, 2003, Pages

  Meyer, Tobias N ^a   Hunt, Jennifer ^a   Schwesinger, Catherine ^b   Denker, Bradley M ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

Adherens junction; G proteins; Rho; Tight junction

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); BETA CATENIN; CELL PROTEIN; DOXYCYCLINE; GENISTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA 12; HETEROTRIMERIC GUANINE NUCLEOTIDE BINDING PROTEIN; HYGROMYCIN; MANNITOL; MEMBRANE PROTEIN; PENICILLIN G; PHOSPHOPROTEIN; PROTEIN KINASE INHIBITOR; PROTEIN KINASE P60; PROTEIN KINASE P60 INHIBITOR; PROTEIN SH3; PROTEIN TYROSINE KINASE INHIBITOR; PROTEIN ZO1; RHO FACTOR; UNCLASSIFIED DRUG; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; PROTEIN TYROSINE KINASE;

ANIMAL CELL; ARTICLE; CELL JUNCTION; CELL STRAIN; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DOWNSTREAM PROCESSING; ENZYME REGULATION; EPITHELIUM CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SIGNAL TRANSDUCTION; TIGHT JUNCTION; ANIMAL; CELL LINE; DOG; ENZYMOLOGY; PHYSIOLOGY;

ANIMALIA;

ADHERENS JUNCTIONS; ANIMALS; CELL LINE; DOGS; EPITHELIAL CELLS; GTP-BINDING PROTEIN ALPHA SUBUNITS, G12-G13; INTERCELLULAR JUNCTIONS; SRC-FAMILY KINASES;

EID: 0142116197     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.00548.2002     Document Type: Article

References (66)

1. Balda MS, Gonzalez-Mariscal L, Macias-Silva M, Torres-Marquez ME, Garcia Sainz JA, and Cereijido M. Assembly and sealing of tight junctions: possible participation of G-proteins, phospholipase C, protein kinase C and calmodulin. J Membr Biol 122: 193-202, 1991.
2. Balda MS and Matter K. Tight junctions. J Cell Sci 111: 541-547, 1998.
3. 13 stimulate Rho-dependent stress fiber formation and focal adhesion assembly. J Biol Chem 270: 24631-24634, 1995.
4. Cereijido M, Ehrenfeld J, Fernandez-Castelo S, and Meza I. Fluxes, junctions, and blisters in cultured monolayers of epithelioid cells (MDCK). Ann NY Acad Sci 372: 422-441, 1981.
5. Chen Y, Lu Q, Schneeberger EE, and Goodenough DA. Restoration of tight junction structure and barrier function by down-regulation of the mitogen-activated protein kinase pathway in ras-transformed Madin-Darby canine kidney cells. Mol Biol Cell 11: 849-862, 2000.
6. Clark EA and Brugge JS. Redistribution of activated pp60c-src to integrin-dependent cytoskeletal complexes in thrombin-stimulated platelets. Mol Cell Biol 13: 1863-1871, 1993.
7. 2-terminal kinase through the small G proteins Ras and Rac. J Biol Chem 271: 17349-17353, 1996.
8. i proteins to specific membrane domains. J Cell Sci 107: 507-515, 1994.
9. Denker BM and Nigam SK. Molecular structure and assembly of the tight junction. Am J Physiol Renal Physiol 274: F1-F9, 1998.
10. Denker BM, Saha C, Khawaja S, and Nigam SK. Involvement of a heterotrimeric G protein α-subunit in tight junction biogenesis. J Biol Chem 271: 25750-25753, 1996.
11. Dodane V and Kachar B. Identification of isoforms of G proteins and PKC that colocalize with tight junctions. J Membr Biol 149: 199-209, 1996.
12. Fanning AS and Anderson JM. PDZ domains: fundamental building blocks in the organization of protein complexes at the plasma membrane. J Clin Invest 103: 767-772, 1999.
13. Fanning AS and Anderson JM. Protein modules as organizers of membrane structure. Curr Opin Cell Biol 11: 432-439, 1999.
14. Fanning AS, Mitic LL, and Anderson JM. Transmembrane proteins in the tight junction barrier. J Am Soc Nephrol 10: 1337-1345, 1999.
15. 12 to the serum response element and to cellular transformation. Proc Natl Acad Sci USA 94: 10098-10103, 1997.
16. Fukuhara S, Murga C, Zohar M, Igishi T, and Gutkind JS. A novel PDZ domain containing guanine nucleotide exchange factor links heterotrimeric G proteins to Rho. J Biol Chem 274: 5868-5879, 1999.
17. 12 mediates signaling from lysophosphatidic acid receptor via epidermal growth factor receptor to Rho. J Biol Chem 273: 4653-4659, 1998.
18. 13 in receptor-mediated stress fiber formation. J Biol Chem 274: 17901-17907, 1999.
19. Gomez S, del Mont Llosas M, Verdu J, Roura S, Lloreta J, Fabre M, and Garcia de Herreros A. Independent regulation of adherens and tight junctions by tyrosine phosphorylation in Caco-2 cells. Biochim Biophys Acta 1452: 121-132, 1999.
20. Gonzalez-Mariscal L, Contreras RG, Bolivar JJ, Ponce A, Chavez De Ramirez B, and Cereijido M. Role of calcium in tight junction formation between epithelial cells. Am J Physiol Cell Physiol 259: C978-C986, 1990.
21. i1, -2, and -3 in polarized epithelial MDCK cells. Biochem Biophys Res Commun 234: 1-7, 1997.
22. Itoh M, Furuse M, Morita K, Kubota K, Saitou M, and Tsukita S. Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins. J Cell Biol 147: 1351-1363, 1999.
23. 12. FEBS Lett 330: 319-322, 1993.
24. 12 stimulates Bruton's tyrosine kinase and a rasGAP through a conserved PH/BM domain. Nature 395: 808-813, 1998.
25. Jou TS and Nelson WJ. Effects of regulated expression of mutant RhoA and Rac1 small GTPases on the development of epithelial (MDCK) cell polarity. J Cell Biol 142: 85-100, 1998.
26. Jou TS, Schneeberger EE, and Nelson WJ. Structural and functional regulation of tight junctions by RhoA and Rac1 small GTPases. J Cell Biol 142: 101-115, 1998.
27. q induce Rho-dependent neurite retraction through different signaling pathways. J Biol Chem 273: 28700-28707, 1998.
28. 13 results in shape change and Rho/Rho-kinase-mediated myosin light chain phosphorylation in mouse platelets. J Cell Biol 144: 745-754, 1999.
29. 13. Science 280: 2109-2111, 1998.
30. Leighton J, Brada Z, Estes LW, and Justh G. Secretory activity and oncogenicity of a cell line (MDCK) derived from canine kidney. Science 163: 472-473, 1969.
31. 12-induced cytoskeletal responses. J Biol Chem 274: 26815-26821, 1999.
32. 12/13. EMBO J 17: 5638-5646, 1998.
33. Martinez-Estrada OM, Villa A, Breviario F, Orsenigo F, Dejana E, and Bazzoni G. Association of junctional adhesion molecule with calcium/calmodulin-dependent serine protein kinase (CASK/LIN-2) in human epithelial Caco-2 cells. J Biol Chem 276: 9291-9296, 2001.
34. McCarthy KM, Skare IB, Stankewich MC, Furuse M, Tsukita S, Rogers RA, Lynch RD, and Schneeberger EE. Occludin is a functional component of the tight junction. J Cell Sci 109: 2287-2298, 1996.
35. 13 negatively regulate the adhesive functions of cadherin. J Biol Chem 277: 24594-24600, 2002.
36. 13 with the cytoplasmic domain of cadherin provides a mechanism for β-catenin release. Proc Natl Acad Sci USA 98: 519-524, 2001.
37. 12 directly binds to the Src homology 3 domain and regulates paracellular permeability in epithelial cells. J Biol Chem 277: 24855-24858, 2002.
38. Meyer TN, Schwesinger C, Ye J, Denker BM, and Nigam SK. Reassembly of the tight junction after oxidative stress depends on tyrosine kinase activity. J Biol Chem 276: 22048-22055, 2001.
39. Mitic LL and Anderson JM. Molecular architecture of tight junctions. Annu Rev Physiol 60: 121-142, 1998.
40. Mullin JM, Kampherstein JA, Laughlin KV, Clarkin CE, Miller RD, Szallasi Z, Kachar B, Soler AP, and Rosson D. Overexpression of protein kinase Cδ increases tight junction permeability in LLC-PK1 epithelia. Am J Physiol Cell Physiol 275: C544-C554, 1998.
41. 12. Oncogene 18: 4425-4434, 1999.
42. Neer EJ. Heterotrimeric G proteins: organizers of transmembrane signals. Cell 80: 249-257, 1995.
43. Patrie KM, Drescher AJ, Welihinda A, Mundel P, and Margolis B. Interaction of two actin-binding proteins, synaptopodin and α-actinin-4, with the tight junction protein MAGI-1. J Biol Chem 277: 30183-30190, 2002.
44. 13 activates a phospholipase D isozyme by a pathway requiring Rho family GTPases. J Biol Chem 273: 4823-4826, 1998.
45. Postma FR, Hengeveld T, Alblas J, Giepmans BN, Zondag GC, Jalink K, and Moolenaar WH. Acute loss of cell-cell communication caused by G protein-coupled receptors: a critical role for c-Src. J Cell Biol 140: 1199-1209, 1998.
46. 13. J Biol Chem 270: 18655-18659, 1995.
47. Ren XD, Kiosses WB, and Schwartz MA. Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton. EMBO J 18: 578-585, 1999.
48. Rosson D, O'Brien TG, Kampherstein JA, Szallasi Z, Bogi K, Blumberg PM, and Mullin JM. Protein kinase C-α activity modulates transepithelial permeability and cell junctions in the LLC-PK1 epithelial cell line. J Biol Chem 272: 14950-14953, 1997.
49. s stimulates TJ assembly. Biochem Biophys Res Commun 285: 250-256, 2001.
50. i2 in the maintenance and biogenesis of epithelial cell tight junctions. J Biol Chem 273: 21629-21633, 1998.
51. Schindler T, Sicheri F, Pico A, Gazit A, Levitzki A, and Kuriyan J. Crystal structure of Hck in complex with a Src family-selective tyrosine kinase inhibitor. Mol Cell 3: 639-648, 1999.
52. 2+ resorption. Science 285: 103-106, 1999.
53. Slice LW, Bui L, Mak C, and Walsh JH. Differential regulation of COX-2 transcription by Ras- and Rho-family of GTPases. Biochem Biophys Res Commun 276: 406-410, 2000.
54. 13 subunits define a fourth class of G protein α-subunits. Proc Natl Acad Sci USA 88: 5582-5586, 1991.
55. Stuart RO, Sun A, Panichas M, Hebert SC, Brenner BM, and Nigam SK. Critical role for intracellular calcium in tight junction biogenesis. J Cell Physiol 159: 423-433, 1994.
56. Takeda H and Tsukita S. Effects of tyrosine phosphorylation on tight junctions in temperature-sensitive v-src-transfected MDCK cells. Cell Struct Funct 20: 387-393, 1995.
57. Tanaka A and Fujita DJ. Expression of a molecularly cloned human c-src oncogene by using a replication-competent retroviral vector. Mol Cell Biol 6: 3900-3909, 1986.
58. Thomas SM and Brugge JS. Cellular functions regulated by Src family kinases. Annu Rev Cell Dev Biol 13: 513-609, 1997.
59. Tsukamoto T and Nigam SK. Role of tyrosine phosphorylation in the reassembly of occludin and other tight junction proteins. Am J Physiol Renal Physiol 276: F737-F750, 1999.
60. Tsukita S, Furuse M, and Itoh M. Multifunctional strands in tight junctions. Nat Rev Mol Cell Biol 2: 285-293, 2001.
61. Tsukita S, Oishi K, Akiyama T, Yamanashi Y, Yamamoto T, and Tsukita S. Specific proto-oncogenic tyrosine kinase of Src family are enriched in cell-to-cell adherens junctions where the level of tyrosine phosphorylation is elevated. J Cell Biol 113: 867-879, 1991.
62. Van Itallie C, Rahner C, and Anderson JM. Regulated expression of claudin-4 decreases paracellular conductance through a selective decrease in sodium permeability. J Clin Invest 107: 1319-1327, 2001.
63. 13 proteins induce neoplastic transformation of Rat-1 fibroblasts. Oncogene 9: 2559-2565, 1994.
64. 12. J Biol Chem 272: 28829-28832, 1997.
65. Walsh SV, Hopkins AM, Chen J, Narumiya S, Parkos CA, and Nusrat A. Rho kinase regulates tight junction function and is necessary for tight junction assembly in polarized intestinal epithelia. Gastroenterology 121: 566-579, 2001.
66. 12 potentiates the eicosanoid pathway and is highly oncogenic in NIH 3T3 cells. Proc Natl Acad Sci USA 90: 6741-6745, 1993.