Caspase-3-mediated cleavage of Cdc6 induces nuclear localization of p49-truncated Cdc6 and apoptosis

Molecular Biology of the Cell

Volumn 14, Issue 10, 2003, Pages 4250-4259

  Yim, Hyungshin ^a   Jin, Ying Hua ^a   Park, Byoung Duck ^a   Choi, Hye Jin ^a   Lee, Seung Ki ^a  

^a Seoul National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE 3; CELL CYCLE PROTEIN; CELL CYCLE PROTEIN 6; CYCLIN DEPENDENT KINASE 2; ETOPOSIDE; GINSENOSIDE; GINSENOSIDE RH 2; PACLITAXEL; TUMOR NECROSIS FACTOR RELATED APOPTOSIS INDUCING LIGAND; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; APOPTOSIS; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL NUCLEUS; CELLULAR DISTRIBUTION; DNA REPLICATION; ENZYME ACTIVITY; HELA CELL; PRIORITY JOURNAL; PROTEIN TRANSPORT;

ACTIVE TRANSPORT, CELL NUCLEUS; AMINO ACID MOTIFS; ANNEXIN A5; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; CASPASE 3; CASPASES; CDC2-CDC28 KINASES; CELL MEMBRANE; CELL NUCLEUS; CYCLIN-DEPENDENT KINASE 2; DNA REPLICATION; ETOPOSIDE; FLOW CYTOMETRY; GINSENOSIDES; HELA CELLS; HUMANS; MEMBRANE GLYCOPROTEINS; NUCLEAR LOCALIZATION SIGNALS; PACLITAXEL; PHOSPHORYLATION; TNF-RELATED APOPTOSIS-INDUCING LIGAND; TUMOR CELLS, CULTURED; TUMOR NECROSIS FACTOR-ALPHA;

EID: 0141764727     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.E03-01-0029     Document Type: Article

References (32)

1. Bermejo, R., Vilaboa, N., and Cales, C. (2002). Regulation of Cdc6, geminin, and Cdt1 in human cells that undergo polyploidization. Mol. Biol. Cell 13, 3989-4000.
2. Blanchard, F., Rusiniak, M.E., Sharma, K., Sun, X., Todorov, I., Castellano, M.M., Gutierrez, C., Baumann, H., and Burhans, W.C. (2002). Targeted destruction of DNA replication protein Cdc6 by cell death pathways in mammals and yeast. Mol. Biol. Cell 13, 1536-1549.
3. Buckley, C.D., Pilling, D., Henriquez, N.V., Parsonage, G., Threlfall, K., Scheel-Toellner, D., Simmons, D.L., Akbar, A.N., Lord, J.M., and Salmon, M. (1999). RGD peptides induce apoptosis by direct caspase-3 activation. Nature 397, 534-539.
4. Casenghi, M., Mangiacasale, R., Tuynder, M., Caillet-Fauquet, P., Elhajouji, A., Lavia, P., Mousset, S., Kirsch-Volders, M., and Cundari, E. (1999). p53-independent apoptosis and p53-dependent block of DNA re-replication following mitotic spindle inhibition in human cells. Exp. Cell. Res. 250, 339-350.
5. Castellano, M.M., del-Pozo, J.C., Ramirez-Para, E., Brown, S., and Gutierrez, C. (2001). Expression and stability of Arabidopsis Cdc6 are associated with endoreplication. Plant Cell 13, 2671-2686.
6. Dalton, S., and Whitbread, L. (1995). Cell cycle-regulated nuclear import and export of Cdc47, a protein essential for initiation of DNA replication in budding yeast. Proc. Natl. Acad. Sci. USA 92, 2514-2518.
7. Delmolino, L.M., Saha, P., and Dutta, A. (2001). Multiple mechanisms regulate subcellular localization of human Cdc6. J. Biol. Chem. 276, 26947-26954.
8. Earnshaw, W.C., Martins, L.M., and Kaufmann, S.H. (1999). Mammalian caspases: structure, activation, substrates, and functions during apoptosis. Annu. Rev. Biochem. 68, 383-424.
9. Guo, M., and Hay, B.A. (1999). Cell proliferation and apoptosis. Curr. Opin. Cell. Biol. 11, 745-752.
10. Hakem, A., Sasaki, T., Kozieradzki, I., and Penninger, J.M. (1999). The cyclin-dependent kinase 2 regulates thymocyte apoptosis. J. Exp. Med. 189, 957-968.
11. Herbig, U., Marlar, C.A., and Fanning, E. (1999). The Cdc6 nucleotide-binding site regulates its activity in DNA replication in human cells. Mol. Biol. Cell 10, 2631-2645.
12. Hong, F.D., Chen, J., Dinican, S., Schneider, N., and Nisen, P.D. (1999). Taxol, vinctistine or nocodazole induces lethality in G1-checkpoint-defective human astrocytoma U353MG cells by triggering hyperploid progression. Carcinogeneis 20, 1161-1168.
13. Jiang, W., Wells, N.J., and Hunter, T. (1999). Multistep regulation of DNA replication by cdk phosphorylation of Hscdc6. Proc. Natl. Acad. Sci. USA 96, 6193-6198.
14. WAF1/CIP1 associated with the cyclin A-cyclin-dependent kinase 2 complex is a prerequisite for apoptosis in SK-HEP-1 cells. J. Biol. Chem. 275, 30256-30263.
15. King, K.L., and Cidlowski, J.A. (1998). Cell cycle regulation and apoptosis. Annu. Rev. Physiol. 60, 601-17.
16. Kirsch-Volders, M., Cundari, E., and Verdoodt, B. (1998). Towards a unifying model for the metaphase/anaphase transition. Mutagenesis 13, 321-335.
17. Lei, M., Kawasaki, Y., and Tye, B.K. (1996). Physical interactions among Mcm proteins and effects of Mcm dosage on DNA replication in Saccharomyces cerevisiae. Mol. Cell. Biol. 16, 5081-5090.
18. KiP1 mediates apoptosis in endothelial cells through activation of Cdk 2, role of a caspase cascade. Mol. Cell 1, 553-563.
19. Liang, C., and Stillman, B. (1997). Persistent initiation of DNA replication and chromatin-bound Mcm proteins during the cell cycle in Cdc6 mutants. Genes Dev. 11, 3375-3386.
20. cdc18 plays a major role controlling the initiation of DNA replication in fission yeast. Cell 83, 397-405.
21. WAF1/CIP1 in the earlier stage of apoptosis in SK-HEP-1 human hepatoma cells. Eur. J. Biochem. 257, 242-249.
22. Pelizon, C. (2003). Down to the origin: Cdc6 protien and the competence to replication. Trends Cell Biol. 13, 110-113.
23. Pelizon, C., Fagagna, F.A., Farrace, L., and Laskey, R.A. (2002). Human replication protein Cdc6 is selectively cleaved by caspase-3 during apoptosis. EMBO Rep. 3, 780-784.
24. Petersen, B.O., Lukas, J., Sorensen, C.S., Bartek, J., and Helin, K. (1999). Phosphorylation of mammalian Cdc6 by cyclin A/Cdk2 regulates its subcellular localization. EMBO J. 18, 396-410.
25. Piatti, S., Lengauer, C., and Nasmyth, K. (1995). Cdc6 is an unstable protein whose de novo synthesis in G1 is important for the onset of S phase and for preventing a 'reductional' anaphase in the budding yeast Saccharomyces cerevisiae. EMBO J. 14, 3788-3799.
26. Sambrook, J., and Russell, D.W. (2001). Molecular Cloning, 3rd ed., Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 13.31-13.35
27. cdc2 activation is required for apoptosis. Science 263, 1143-1145.
28. Takayama, M., Taira, T., Iguchi-Ariga, S.M.M., and Ariga, H. (2000). Cdc6 interacts with c-Myc to inhibit E-box-dependent transcription by abrogating c-Myc/Max complex. FEBS Lett. 477, 43-48.
29. Takei, Y., Yamamoto, K., and Tsujimoto, G. (1999). Identification of the sequence responsible for the nuclear localization of human cdc6. FEBS Lett, 447, 292-296.
30. Verdoodt, B., Decordier, I., Geleyns, K., Cunha, M., Cundari, E., and Kirsch-Volders, M. (1999). Induction of polyploidy and apoptosis after exposure to high concentrations of the spindle poison nocodazole. Mutagenesis 14, 513-520.
31. Williams, R.S., Shohet, R.V., and Stillman, B. (1997). A human protein related to yeast Cdc6p. Proc. Natl. Acad. Sci. USA 94, 142-147.
32. Yan, Z., DeGregori, J., Shohet, R., Leone, G., Stillman, B., Nevins, J.R., and Williams, R.S. (1998). Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells. Proc. Natl. Acad. Sci. USA 95, 3603-3608.