The Cdc6 nucleotide-binding site regulates its activity in DNA replication in human cells

Molecular Biology of the Cell

Volumn 10, Issue 8, 1999, Pages 2631-2645

  Herbig, Utz ^a   Marlar, Clinton A ^a   Fanning, Ellen ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; DNA; NUCLEOTIDE;

ARTICLE; BINDING SITE; CELL CYCLE S PHASE; COMPLEX FORMATION; CONTROLLED STUDY; DNA REPLICATION; ENZYME ACTIVITY; GENE MUTATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN DEGRADATION; REGULATORY MECHANISM;

SACCHAROMYCETALES;

EID: 0032807099     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.10.8.2631     Document Type: Article

References (46)

1. Araki, T., Yamamoto, A., and Yamada, M. (1987). Accurate determination of DNA content in single cell nuclei stained with Hoechst 33258 fluorochrome at high salt concentration. Histochemistry 87, 331-338.
2. Baker, I.A., and Bell, S.P. (1998). Polymerases and the replisome: machines within machines. Cell 92, 295-305.
3. Brown, G.W., Jallepalli, P.V., Huneycutt, B.J., and Kelly, T.J. (1997). Interaction of the S phase regulator cdc18 with cyclin-dependent kinase in fission yeast. Proc. Natl. Acad. Sci. USA 94, 6142-6147.
4. Cocker, J.H., Piatti, S., Santocanale, C., Nasmyth, K., and Diffley, J.F. (1996). An essential role for the Cdc6 protein in forming the prereplicative complexes of budding yeast. Nature 379, 180-182.
5. Coleman, T.R., Carpenter, P.B., and Dunphy, W.G. (1996). The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts. Cell 87, 53-63.
6. + gene product. Genetics 151, 1445-1457.
7. Detweiler, C.S., and Li, J.J. (1997). Cdc6p establishes and maintains a state of replication competence during G1 phase. J. Cell Sci. 110, 753-763.
8. Diffley, J.F., and Cocker, J.H. (1992). Protein-DNA interactions at a yeast replication origin. Nature 357, 169-172.
9. Diffley, J.F., Cocker, J.H., Dowell, S.J., and Rowley, A. (1994). Two steps in the assembly of complexes at yeast replication origins in vivo. Cell 78, 303-316.
10. Donovan, S., Harwood, J., Drury, L.S., and Diffley, J.F. (1997). Cdc6p-dependent loading of Mem proteins onto prereplicative chromatin in budding yeast. Proc. Natl. Acad. Sci. USA 94, 5611-5616.
11. Drury, L.S., Perkins, G., and Diffley, J.F. (1997). The Cdc4/34/53 pathway targets Cdc6p for proteolysis in budding yeast. EMBO J. 16, 5966-5976.
12. Dutta, A., and Bell, S.P. (1997). Initiation of DNA replication in eukaryotic cells. Annu. Rev. Cell Dev. Biol. 13, 293-332.
13. Elsasser, S., Lou, F., Wang, B., Campbell, J.L., and Jong, A. (1996). Interaction between yeast Cdc6 protein and B-type cyclin/Cdc28 kinases. Mol. Biol. Cell 7, 1723-1735.
14. Hateboer, G., Wobst, A., Petersen, B.O., Le Cam, L., Vigo, E., Sardet, C., and Helin, K. (1998). Cell cycle-regulated expression of mammalian CDC6 is dependent on E2F. Mol. Cell. Biol. 18, 6679-6697.
15. Hua, X.H., and Newport, J. (1998). Identification of a preinitiation step in DNA replication that is independent of origin recognition complex and cdc6, but dependent on cdk2. J. Cell Biol. 140, 271-281.
16. cdc18 by CDK phosphorylation. Genes Dev. 11, 2767-2779.
17. Jallepalli, P.V., Tien, D., and Kelly, T.J. (1998). sud1(+) targets cyclin-dependent kinase-phosphorylated Cdc18 and Rum1 proteins for degradation and stops unwanted diploidization in fission yeast. Proc. Natl. Acad. Sci. USA 95, 8159-8164.
18. Johnson, D.G., and Nogueira Araujo, G.M. (1981). A simple method of reducing the fading of immunofluorescence during microscopy. J. Immunol. Methods 43, 349-350.
19. Kelly, T.J., Martin, G.S., Forsburg, S.L., Stephen, R.J., Russo, A., and Nurse, P. (1993). The fission yeast cdc18+ gene product couples S phase to START and mitosis. Cell 74, 371-382.
20. Klemm, R.D., Austin, R.J., and Bell, S.P. (1997). Coordinate binding of ATP and origin DNA regulates the ATPase activity of the origin recognition complex. Cell 88, 493-502.
21. Kominami, K., and Toda, T. (1997). Fission yeast WD-repeat protein pop1 regulates genome ploidy through ubiquitin-proteasome-mediated degradation of the CDK inhibitor Rum1 and the S-phase initiator Cdc18. Genes Dev. 11, 1548-1560.
22. Koonin, E.V. (1993). A common set of conserved motifs in a vast variety of putative nucleic acid-dependent ATPases including MCM proteins involved in the initiation of eukaryotic DNA replication. Nucleic Acids Res. 21, 2541-2547.
23. Leatherwood, J., Lopez-Girona, A., and Russell, P. (1996). Interaction of Cdc2 and Cdc18 with a fission yeast ORC2-like protein. Nature 379, 360-363.
24. Li, J.J., and Herskowitz, I. (1993). Isolation of ORC6, a component of the yeast origin recognition complex by a one-hybrid system. Science 262, 1870-1874.
25. Liang, C., Weinreich, M., and Stillman, B. (1995). ORC and Cdc6p interact and determine the frequency of initiation of DNA replication in the genome. Cell 81, 667-676.
26. Lopez-Girona, A., Mondesert, O., Leatherwood, J., and Russell, P. (1998). Negative regulation of Cdc18 DNA replication protein by Cdc2. Mol. Biol. Cell 9, 63-73.
27. Muzi Falconi, M., Brown, G.W., and Kelly, T.J. (1996). cdc18+ regulates initiation of DNA replication in Schizosaccharomyces pombe. Proc. Natl. Acad. Sci. USA 93, 1566-1570.
28. Newlon, C.S. (1997). Putting it all together: building a prereplicative complex. Cell 91, 717-720.
29. Perkins, G., and Diffley, J.F. (1998). Nucleotide-dependent prereplicative complex assembly by Cdc6p, a homolog of eukaryotic and prokaryotic clamp-loaders. Mol. Cell 2, 23-32.
30. Petersen, B.O., Lukas, J., Sorensen, C.S., Bartek, J., and Helin, K. (1999). Phosphorylation of mammalian CDC6 by cyclin A/CDK2 regulates its subcellular localization. EMBO J. 18, 396-410.
31. Piatti, S., Lengauer, C., and Nasmyth, K. (1995). Cdc6 is an unstable protein whose de novo synthesis in G1 is important for the onset of S phase and for preventing a "reductional" anaphase in the budding yeast Saccharomyces cerevisiae. EMBO J. 14, 3788-3799.
32. Podust, V.N., Tiwari, N., Ott, R., and Fanning, E. (1998). Functional interactions among the subunits of replication factor C potentiate and modulate its ATPase activity. J. Biol. Chem. 273, 12935-12942.
33. Saha, P., Chen, J., Thome, K.C., Lawlis, S.J., Hou, Z.H., Hendricks, M., Parvin, J.D., and Dutta, A. (1998). Human CDC6/Cdc18 associates with Orc1 and cyclin-cdk and is selectively eliminated from the nucleus at the onset of S phase. Mol. Cell. Biol. 18, 2758-2767.
34. Santocanale, C., and Diffley, J.F. (1996). ORC- and Cdc6-dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae. EMBO J. 15, 6671-6679.
35. Saraste, M., Sibbald, P.R., and Wittinghofer, A. (1990). The P-loop - a common motif in ATP- and GTP-binding proteins. Trends Biochem. Sci. 15, 430-434.
36. Stillman, B. (1996). Comparison of DNA replication in cells from prokaryotes and eukaryotes. In: DNA Replication in Eukaryotic Cells, ed. M.L. DePamphilis, Cold Spring Harbor, NY: CSH Press, 435-460.
37. Story, R.M., and Steitz, T.A. (1992). Structure of the recA protein-ADP complex. Nature 355, 374-376.
38. Tanaka, T., Knapp, D., and Nasmyth, K. (1997). Loading of an MCM protein onto DNA replication origins is regulated by Cdc6p and CDKs. Cell 90, 649-660.
39. Waga, S., and Stillman, B. (1998). The DNA replication fork in eukaryotic cells. Annu. Rev. Biochem. 67, 721-751.
40. Wahle, E., Lasken, R.S., and Kornberg, A. (1989). The dnaB-dnaC replication protein complex of Escherichia coli. I. Formation and properties. J. Biol. Chem. 264, 2463-2468.
41. Walker, J.E., Saraste, M., Runswick, M.J., and Gay, N.J. (1982). Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold. EMBO J. 1, 945-951.
42. Wang, B., Feng, L., Hu, Y., Huang, S.H., Reynolds, C.P., Wu, L., and Jong, A.Y. (1999). The essential role of Saccharomyces cerevisiae CDC6 nucleotide-binding site in cell growth, DNA synthesis, and Orc1 association. J. Biol. Chem. 274, 8291-8298.
43. Weinreich, M., Liang, C., and Stillman, B. (1999). The Cdc6p nucleotide-binding motif is required for loading Mem proteins onto chromatin. Proc. Natl. Acad. Sci. USA 96, 441-446.
44. Williams, R.S., Shohet, R.V., and Stillman, B. (1997). A human protein related to yeast Cdc6p. Proc. Natl. Acad. Sci. USA 94, 142-147.
45. Yan, Z., DeGregori, J., Shohet, R., Leone, G., Stillman, B., Nevins, J.R., and Williams, R.S. (1998). Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells. Proc. Natl. Acad. Sci. USA 95, 3603-3608.
46. Zwerschke, W., Rottjakob, H.W., and Kuntzel, H. (1994). The Saccharomyces cerevisiae CDC6 gene is transcribed at late mitosis and encodes a ATP/GTPase controlling S phase initiation. J. Biol. Chem. 269, 23351-23356.