Optimization of reverse transcriptase PCR to detect viable Shiga-toxin-producing Escherichia coli

Applied and Environmental Microbiology

Volumn 68, Issue 2, 2002, Pages 799-806

  McIngvale, S C ^a   Elhanafi, D ^a   Drake, M A ^a  

^a NORTH CAROLINA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; DNA; ELECTROPHORESIS; OPTIMIZATION; RNA;

AERATION;

ESCHERICHIA COLI;

BACTERIAL DNA; BACTERIAL RNA; SHIGA TOXIN;

BACTERIUM;

AERATION; ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM DETECTION; CULTURE MEDIUM; DNA DETERMINATION; ESCHERICHIA COLI; FOOD CONTAMINATION; GEL ELECTROPHORESIS; MEAT; NONHUMAN; OPERON; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SENSITIVITY AND SPECIFICITY; SOUTHERN BLOTTING; TEMPERATURE; TOXIN ANALYSIS;

ANIMALS; CATTLE; CULTURE MEDIA; DNA PRIMERS; DNA, BACTERIAL; ESCHERICHIA COLI O157; MEAT PRODUCTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, MESSENGER; SHIGA TOXIN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0036157063     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.2.799-806.2002     Document Type: Article

References (35)

1. How does Escherichia coli O157:H7 testing in meat compare with what we are seeing clinically?
2. Genetic and immunological techniques for detecting foodborne pathogens and toxins
3. Transcriptional regulation of the esp genes of enterohemorrhagic Escherichia coli
4. Polymerase chain reaction (PCR) detection of Lysteria monocytogenes in diluted milk and reversal of PCR inhibition caused by calcium ions
5. Detection of verotoxigenic Escherichia coli by magnetic capture-hybridization PCR
6. Persistence of Escherichia coli O157:H7 in dairy fermentation systems
7. Isolation of Escherichia coli O157:H7 from retail fresh meats and poultry
8. Escherichia coli 0157:H7
9. Genetic analysis for virulence factors in Escherichia coli O104:H21 that was implicated in an outbreak of hemorrhagic colitis
10. Simultaneous detection of Salmonella spp. and Escherichia coli O157:H7 by multiplex PCR
11. Use of flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains
12. Detection of viable and dead Listeria monocytogenes by PCR
13. Influence of oxygen availability on physiology, verocytotoxin expression and adherence of Escherichia coli O157
14. PCR amplification of Shiga-like toxin genes
15. Sensitive detection of viable Listeria monocytogenes by reverse transcription-PCR
16. Survival of Escherichia coli O157:H7 in buttermilk as affected by contamination point and storage temperature
17. rRNA stability in heat-killed and UV-irradiated enterotoxigenic Staphylococcus aureus and Escherichia coli O157:H7
18. Nucleic acid persistence in heat-killed Escherichia coli O157:H7 from contaminated skim milk
19. A comparison of methods for the detection of Escherichia coli O157:H7 from artificially contaminated dairy products using PCR
20. A multiplex PCR for identifying Shiga-like toxin-producing Escherichia coli O157:H7
21. Cloning and sequencing of a shiga-like toxin II-related gene from Escherichia coil O157:H7 strain 7279
22. Regulation of the Shigalike toxin II operon in Escherichia coli
23. Shiga and Shiga-like toxins
24. Minimum and maximum temperatures for growth and verotoxin production by hemorrhagic strains of Escherichia coli
25. Antibodydirect epifluorescent filter technique and immunomagnetic separation of 10-h screening and 24-h confirmation of Escherichia coli 0157:H7 in beef
26. Variants of shiga-like toxin II constitute a major toxin component in Escherichia coli O157 strains from patients with haemolytic uremic syndrome
27. Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells
28. Transcription of the Shiga-like toxin type II and Shiga-like toxin type II variant operons of Escherichia coli
29. Method to isolate Escherichia coli O157:H7 from food
30. Comparison of methods for determining the presence of Escherichia coli O157:H7 in apple juice
31. Survival of Escherichia Esche?ichia coli O157 0157:H7 and Salmonella ssp. in acidic condiments
32. Detection of Escherichia coli O157:H7 and other verocytotoxin-producing E. coli (VTEC) in food
33. An improved rapid technique for isolation of Escherichia coli O157:H2 from foods
34. Detection and production of verotoxin I of Escherichia coli O157:H7 in food
35. Inhibition and facilitation of nucleic acid amplification