A PCR-ELISA for detecting Shiga toxin-producing Escherichia coli

Microbes and Infection

Volumn 4, Issue 3, 2002, Pages 285-290

  Ge, Beilei ^a   Zhao, Shaohua ^a   Hall, Robert ^b   Meng, Jianghong ^a  

^a UNIVERSITY OF MARYLAND   (United States)

^b CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

ELISA; PCR; Shiga toxin producing E. coli

Indexed keywords

BIOTIN; DIGOXIGENIN; SHIGA TOXIN; STREPTAVIDIN;

ACCURACY; ANALYTIC METHOD; ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; CONTROLLED STUDY; DNA EXTRACTION; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; ESCHERICHIA COLI K 12; GEL ELECTROPHORESIS; GENOTYPE; MEAT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SALMONELLA; SEROTYPE;

ANIMALS; BASE SEQUENCE; DNA, BACTERIAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FOOD MICROBIOLOGY; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SHIGA TOXIN;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; SALMONELLA;

EID: 0036219513     PISSN: 12864579     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1286-4579(02)01540-X     Document Type: Article

References (24)

1. Hemorrhagic colitis associated with a rare Escherichia coli serotype
2. Escherichia coli O157:H7 and other enterohemorrhagic Escherichia coli
3. Microbiology of Shiga toxin-producing Escherichia coli in food
4. Food-related illness and death in the United States
5. Polymerase chain reaction for diagnosis of enterohemorrhagic Escherichia coli infection and hemolytic-uremic syndrome
6. Detection and characterization of the eae gene of Shiga-like toxin-producing Escherichia coli using polymerase chain reaction
7. Identification of Escherichia coli serotype O157:H7 by DNA probe specific for an allele of uid A gene
8. Polymerase chain reaction for detecting Escherichia coli O157:H7
9. An enzyme-linked immunosorbent assay to detect PCR products of the rfbS gene from serogroup D salmonellae: A rapid screening prototype
10. Structure, biology, and relative toxicity of Shiga toxin family members for cells and animals
11. Direct detection of verotoxin-producing Escherichia coli in stool samples by PCR
12. PCR assay for detection of the E. coli O157:H7 eae-gene and effect of the sample preparation method on PCR detection of heat-killed E. coli O157:H7 in ground beef
13. Preparation of PCR samples from food by a rapid and simple centrifugation technique evaluated by detection of Escherichia coli O157:H7
14. Sensitivity and specificity of a commercially available enzyme-linked immunoassay for the detection of polymerase chain reaction amplified DNA
15. A quantitative PCR-ELISA for the rapid enumeration of bacteria in refrigerated raw milk
16. Accuracy of genotyping of single-nucleotide polymorphisms by PCR-ELISA allele-specific oligonucleotide hybridization typing and by amplification refractory mutation system
17. Rapid detection of Listeria monocytogenes by PCR-ELISA
18. Development and evaluation of a PCR-microplate capture hybridization method for direct detection of verotoxigenic Escherichia coli strains in artificially contaminated food samples
19. Rapid enumeration of Escherichia coli in oysters by a quantitative PCR-ELISA
20. Virulence genes of Shiga toxin-producing Escherichia coli isolated from food, animals and humans
21. A multiplex polymerase chain reaction assay for rapid detection and identification of Escherichia coli O157:H7 in foods and bovine feces