Effects of flavonoid extract Enzogenol® with vitamin C on protein oxidation and DNA damage in older human subjects

Nutrition Research

Volumn 23, Issue 9, 2003, Pages 1199-1210

  Senthilmohan, Senti T ^a   Zhang, Jingli ^b   Stanley, Roger A ^b  

^a UNIVERSITY OF CANTERBURY   (New Zealand)

^b THE NEW ZEALAND INSTITUTE FOR PLANT AND FOOD RESEARCH LIMITED   (New Zealand)

Author keywords

Antioxidant; DNA damage; Enzogenol ; Flavonoid; Pine bark; Proanthocyanidin; Protein oxidation

Indexed keywords

ASCORBIC ACID; CARBONYL DERIVATIVE; ENZOGENOL; FLAVONOID; MARKER; PLANT EXTRACT; PROANTHOCYANIDIN; PROTEIN; UNCLASSIFIED DRUG;

ADULT; AGED; ALKALINITY; ANALYSIS; ARTICLE; BLOOD ANALYSIS; BLOOD SAMPLING; CELL ISOLATION; CLINICAL ARTICLE; CLINICAL TRIAL; COMET ASSAY; COMMERCIAL PHENOMENA; CONCENTRATION (PARAMETERS); CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DERIVATIZATION; DNA DAMAGE; DRUG EFFECT; DRUG FORMULATION; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; MALE; MONONUCLEAR CELL; OXIDATION; OXIDATIVE STRESS; PINE; PINUS RADIATA; PRIORITY JOURNAL; SUPPLEMENTATION;

PINUS RADIATA;

EID: 0042236910     PISSN: 02715317     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0271-5317(03)00127-1     Document Type: Article

References (54)

1. .-. Am J Physiol 1995;268:L699.
2. Griffiths HR, Lunec J. Molecular aspects of free radical damage in inflammatory autoimmune pathology. In: Aruoma OI, Halliwell B., editors. Molecular Biology of Free Radicals in Human Diseases, 1998, OICA International, pp. 327-366.
3. Harman D. The aging process. Proc Natl Acad Sci USA 1981;78:7124-8.
4. Gilmour I. Fighting free radicals. In: Duncan K, editor. The Enzogenol Story. Auckland New Zealand: The Pacific Scientific Press, 1998. p. 55-60.
5. Wood JE, Senthilmohan ST, Peskin AV. Antioxidant activity of procyanidin-containing plant extracts at different pHs. Food Chemistry 2002;77:155-61.
6. Ciolino HP, Levine RL. Modification of proteins in endothelial cell death during oxidative stress. Free Rad Biol Med 1997;22:1277-82.
7. Lodish H, Baltimore D, Berk A, Zipursky SL, Matsudaira P, Darnell J. Molecular cell biology, 3rd ed. New York: W H Freeman and Co, 1995.
8. Collins A, Dusinska M, Franklin M, Somorovska M, Petrovska H, Duthie S, Fillion L, Panayiotidis M, Raslova K, Vaughan N. Comet assay in human biomonitoring studies: Reliability, validation, and applications. Environ Mol Mutag 1997;30:139-46.
9. Duthie SJ, Collins AR, Duthie CG, Dobson VL. Quercetin and myricetin protect against hydrogen peroxide induced DNA damage (strand breaks and oxidised pyrimidines) in human lymphocytes. Mutat Res 1997a; 393:223-31.
10. Beatty ER, O'Reilly DO, England TG, McAnlis GT, Young IS, Halliwell B, Geissler CA, Sanders TAB, Wiseman H. Effect of dietary quercetin on oxidative DNA damage in healthy human subjects. Br J Nutr 2000;84:919-25.
11. Middleton E, Kandaswami C. The impact of plant flavonoids on mammalian biology: implications for immunity, inflammation and cancer. In: Harborne JB, editor. The flavonoids: advances in research since 1986. London: Chapman and Hall, 1993. p. 619-52.
12. Middleton E. Biological properties of plant flavonoids: an overview. International J Pharmacog 1996;34:344-48.
13. Duthie SJ, Johnson W, Dobson VL. The effect of dietary flavonoids on DNA damage (strand breaks and oxidised pyrimidines) and growth in human cells. Mutat Res 1997b;390:141-51.
14. Johnson MK, Loo G. Effects of epigallcatechin gallate and quercetin on oxidative damage to cellular DNA. Mutat Res 2000;459:211-18.
15. Markham KR, Porter LJ. Extractives of Pinus radiata bark. N Z J Sci 1973;16:751-61.
16. Porter LJ. Extractives of Pinus radiata bark. N Z J Sci 1974;17:213-18.
17. Packer L, Rimbach G, Virgili F. Antioxidant activity and biologic properties of a procyanidin-rich extract from pine (Pinus maritima) bark, Pycnogenol. Free Rad Biol Med 1999;27:704-24.
18. Jones E, Hughes RE. The influence of bioflavonoids on the absorbtion of vitamin C. IRCS J Med Sci 1984;12:320.
19. Hughes RE, Wilson HK. Flavonoids: some physiological and nutritional considerations. Prog Med Chem 1977;14:285-301.
20. Jones, E. Nutritional Significance of Flavonoids. Ph.D. Thesis 1983;University of Wales, UK.
21. Shand, CB, Strey, C, Scott, R, Morrison, Z, Gieseg, S. Pilot study on the clinical effects of dietary supplementation with Enzogenol® a flavonoid extract of pine bark and vitamin C. Phytother Res. In press.
22. Smith MJ, Inserra PF, Watson RR, Wise JA, O'Neill KL. Supplementation with fruit and vegetable extracts may decrease DNA damage in the peripheral lymphocytes of an elderly population. Nutr Res 1999;19:1507-18.
23. Buss H, Chan TP, Sluis KB, Domigan NM, Winterbourn CC. Protein carbonyl measurement by a sensitive ELISA method. Free Rad Biol Med 1997;233:361-66.
24. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988;175:184-91.
25. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutag 2000;35:206-21.
26. Olive PL, Banath JP. Induction and rejoining of radiation-induced DNA single-strand breaks: "tail moment" as a function of position in the cell cycle. Mutat Res 1993;294(3):275-83.
27. Cao G, Alessio HM, Cutler RG. Oxygen-radical absorbance capacity assay for antioxidants. Free Rad Biol Med 1993;14:303-11.
28. 2) not a gaussian distribution. Mutat Res 1998;398:101-10.
29. Mendoza-Nunez VM, Sanchez-Rodriguez MA, Retana-Ugalde R, Vargas-Guadarrama LA, Altamirano-Lozano MA. Total antioxidant levels, gender, and age as risk factors for DNA damage in lymphocytes of the elderly. Mech Ageing Dev 2001;122:835-47.
30. Winterbourn CC. Nutritional antioxidants: their role in disease prevention. N Z Med J 1995;108:447-48.
31. Block G, Patterson B, Subar A. Fruit vegetable and cancer prevention: a review of the epidemiological evidence. Nutr Cancer 1992;18:1-29.
32. Hertog MLG, Feskens EJM, Hollman PHC, Katan MB, Kromhout D. Dietary antioxidants flavonoids and the risk of coronary heart disease: the zutphen elderly study. Lancet 1993;342:1007-11.
33. Ames BN, Shigenaga M.K. Oxidants are a major contributor to aging. Ann NY Acad Sci 1992;663:85-96.
34. McCall MR, Frei B. Can antioxidant vitamins materially reduce oxidative damage in humans? Free Rad Biol Med 1999;26:1034-53.
35. Carr A, Frei B. Does vitamin C act as a pro-oxidant under physiological conditions? FASEB J 1999;13:1007-24.
36. Anderson D, Phillips BJ, Yu T, Edwards AJ, Ayesh R, Butterworth KR. The effects of vitamin C supplementation on biomarkers of oxygen radical generated damage in human volunteers with "low" or "high" cholesterol levels. Environ Mol Mutag 1997;30:161-74.
37. Singh NP. Sodium ascorbate induces DNA single-strand breaks in human cells in vitro. Mutat Res 1997;375:195-203.
38. Anderson D, Phillips BJ. Comparative in vitro and in vivo effects of antioxidants. Food Chem Toxicol 1999;37:1015-25.
39. Noroozi M, Angerson WJ, Lean MEJ. Effects of flavonoids and vitamin C on oxidative DNA damage to human lymphocytes. Am J Clin Nutr 1998;67:1210-18.
40. Sierens J, Hartley JA, Campbell MJ, Leathem AJC, Woodside JV. Effect of phytoestrogen and antioxidant supplementation on oxidative DNA damage assessed using the comet assay. Mutat Res 2000;485:169-76.
41. Collins BH, Horska A, Hotten PM, Riddoch C, Collins AR. Kiwifruit protects against oxidative damage in human cells and In vitro. Nutr Cancer 2001;39:148-53.
42. Riso P, Pinder A, Santangelo A, Porrini M. Does tomato consumption effectively increase the resistance of lymphocyte DNA to oxidative damage? Am J Clin Nutr 1999;96:712-18.
43. Carty JL, Beavan R, Waller H, Mistry N, Cooke M, Lunec J, Griffiths HR. The effects of vitamin C supplementation on protein oxidation in healthy volunteers. Biochem Biophys Res Comm 2000;273:729-35.
44. Winterboum CC, Chan T, Buss H, Inder TE, Mogridge N, Darlow BA. Protein carbonyls and lipid peroxidation products as oxidation markers in pre-term infant plasma: association with chronic lung disease and retinopathy and effects of selenium supplementation. Pediatr Res 2000;48(1):84-90.
45. Stadtman ER. Metal ion catalyzed oxidation of proteins: Biochemical mechanism and biological consequences. Free Rad Biol Med 1990;9:315-25.
46. Oliver C, Ahn B, Moerman E, Goldstein S, Stadtman E. Age-related changes in oxidized proteins. J Biol Chem 1987;262:5488-91.
47. Sohal RS, Ku HH, Agarwal S, Forster MJ, Lal H. Oxidative damage, mitochondrial oxidant generation and antioxidant defenses during aging and in response to food restriction in the mouse. Mech Ageing Devel 1994;74:121-33.
48. Berlett BS, Stadtman ER. Protein oxidation in aging, disease, and oxidative stress. Biocheml J 1997;324:1-18.
49. Marangon K, Devaraj S, Jialal I. Measurement of protein carbonyls in plasma of smokers and in oxidized LDL by an ELISA. Clin Chem 1999;45(4):577-78.
50. Reznick A, Packer L Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol 1994;233:357-63.
51. Yamakoshi J, Kataoka S, Koga T, Ariga T. Proanthocyanidin-rich extract from grape seeds attenuates the development of aortic atherosclerosis in cholesterol-fed rabbits. Atherosclerosis 1999;142:139-49.
52. Prior R, Cao G. Antioxidant capacity and polyphenolic components of teas: Implications for altering in vivo antioxidant status. Proc Soc Exp Biol Med 1999;220:255-61.
53. Niwa Y, Ishimoto K, Kanoh T. Induction of superoxide dismutase in leukocytes by paraquat: correlation with age and possible predictor of longevity. Blood 1990;76:835-41.
54. Santa Maria C, Machado A. Effect of development and ageing on pulmonary NADPH-cyt C reductase, glutathione peroxidase, glutathione reductase and thioredoxin reductase activities in male and female rats. Mech Ageing Dev 1987;37:183-95.