Chronic regulation of the expression of the gap junction protein connexin 43 in transfected HeLa cells

Naunyn-Schmiedeberg's Archives of Pharmacology

Volumn 368, Issue 1, 2003, Pages 33-40

  Salameh, A ^a   Polontchouk, L ^b   Dhein, S ^b   Hagendorff, A ^a   Pfeiffer, D ^a  

^a UNIVERSITY HOSPITAL LEIPZIG   (Germany)

^b UNIVERSITY OF LEIPZIG   (Germany)

Author keywords

cAMP; Connexin43; Gap junction; MAP kinase; Protein kinase A; Protein kinase C; TNF

Indexed keywords

2 (2 AMINO 3 METHOXYPHENYL)CHROMONE; 4 (4 FLUOROPHENYL) 2 (4 METHYLSULFINYLPHENYL) 5 (4 PYRIDYL)IMIDAZOLE; BUCLADESINE; CONNEXIN 43; CYCLIC AMP; CYCLIC AMP DEPENDENT PROTEIN KINASE; FORSKOLIN; GAP JUNCTION PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE P38; PHORBOL DIDECANOATE; PHORBOL ESTER DERIVATIVE; PROTEIN KINASE C; PROTEIN TYROSINE KINASE; TUMOR NECROSIS FACTOR ALPHA; TUMOR NECROSIS FACTOR ALPHA RECEPTOR; UNCLASSIFIED DRUG;

ARTICLE; CELL COMMUNICATION; CONTROLLED STUDY; ENZYME ACTIVATION; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; HELA CELL; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; POLYMERASE CHAIN REACTION; PROTEIN CONTENT; WESTERN BLOTTING;

ADENYLATE CYCLASE; BLOTTING, WESTERN; CONNEXIN 43; CYCLIC AMP; CYCLIC AMP-DEPENDENT PROTEIN KINASES; ENZYME INHIBITORS; FLAVONOIDS; FLUORESCENT ANTIBODY TECHNIQUE; FORSKOLIN; GAP JUNCTIONS; GENE EXPRESSION REGULATION; HELA CELLS; HUMANS; IMIDAZOLES; PROTEIN KINASE C; PROTEIN-TYROSINE KINASES; PYRIDINES; RECEPTORS, TUMOR NECROSIS FACTOR; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; TRANSFECTION; TUMOR NECROSIS FACTOR-ALPHA;

EID: 0042159891     PISSN: 00281298     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00210-003-0760-1     Document Type: Article

References (51)

1. Alessi DR, Cuenda A, Cohen P, Dudley DT, Saltiel AR (1995) PD098059 is a specific inhibitor of the activation of mitogen-activated protein kinase in vitro and in vivo. J Biol Chem 270: 27489-27494
2. Arensbak B, Mikkelsen HB, Gustafsson F, Christensen T, Holstein-Rathlou N-H (2001) Expression of connexin 37, 40, and 43 mRNA and protein in renal glomerular arterioles. Histochem Cell Biol 115:479-487
3. Bastide B, Neyses L, Ganten D, Paul M, Willecke K, Traub O (1993) Gap junction protein connexin 40 is preferentially expressed in vascular endothelium and conductive bundles of rat myocardium and is increased under hypertensive conditions. Circ Res 73:1138-1149
4. Chevalier D, Allen BG (2000) Two distinct forms of MAPKAP Kinase-2 in adult cardiac ventricular myocytes. Biochemistry 39:6145-6156
5. Christ GJ, Spray DC, El-Sabban M, Moore LK, Brink PR (1996) Gap junctions in vascular tissues. Evaluating the role of intercellular communication in the modulation of vascular tone. Circ Res 79:631-646
6. Cuenda A, Rouse J, Doza YN, Meier R, Cohen P, Gallagher TF, Young PR, Lee JC (1995) SB203580 is a specific inhibitor of a MAP kinase homologue which is stimulated by cellular stresses and interleukin-1. FEBS Lett 364:229-233
7. Cuenda A, Cohen P, Buee-Scherrer V, Goedert M (1997) Activation of stress-activated protein kinase-3 (SAPK3) by cytokines and cellular stresses is mediated via SAPKK3 (MKK6); comparison of the specificities of SAPK3 and SAPK2 (RK/p38). EMBO J 16:295-305
8. Darrow BJ, Laing JG, Lampe PD, Saffitz JE, Beyer EC (1995) Expression of multiple connexins in cultured neonatal rat ventricular myocytes. Circ Res 76:381-387
9. Darrow BJ, Fast VG, Kléber AG, Beyer EC, Saffitz JE (1996) Functional and structural assessment of intercellular communication. Increased conduction velocity and enhanced connexin expression in dibutyryl cAMP-treated cultured cardiac myocytes. Circ Res 79:174-183
10. Davis LM, Rodefeld ME, Green K, Beyer EC, Saffitz JE (1995) Gap junction protein phenotypes of human heart and conduction system. J Cardiovasc Electrophysiol 6:813-822
11. DeLeon JR, Buttrick PM, Fishman GI (1994) Functional analysis of the connexin 43 gene promoter in vivo and in vitro. J Mol Cell Cardiol 26:379-389
12. Dhein S (1998a) Cardiac gap junctions. Physiology, regulation, pathophysiology and pharmacology. Karger, Basel, pp 1-100
13. Dhein S (1998b) Gap junction channels in the cardiovascular system: pharmacological and physiological modulation. Trends Pharmacol Sci 19:229-241
14. Dhein S, Weng S, Schaefer T, Grover R, Polontchouk L (2001) Role of protein kinase C in the action of antiarrhythmic peptides. FASEB J 15 [Suppl]:465
15. Dodge SM, Beardslee MA, Darrow BJ, Green KG, Beyer EC, Saffitz JE (1998) Effects of angiotensin II on expression of the gap junction channel protein connexin 43 in neonatal rat ventricular myocytes. Am J Coll Cardiol 32:800-807
16. Echetebu CO, Ali M, Izban MG, MacKay L, Garfield RE (1999) Localization of regulatory protein binding sites in the proximal region of human myometrial connexin 43 gene. Mol Hum Reprod 5:757-766
17. Eder J (1997) Tumor necrosis factor α and interleukin 1 signalling: do MAPKK kinases connect it all? Trends Pharmacol Sci 18: 319-322
18. English JM, Cobb MH (2002) Pharmacological inhibitors of MAPK pathways. Trends Pharmacol Sci 23:40-45
19. Eugenin EA, Eckardt D, Theis M, Willecke K, Bennett MV, Saez JC (2001) Microglia at brain stab wounds express connexin43 and in vitro form functional gap junctions after treatment with interferon-gamma and tumor necrosis factor-alpha. Proc Natl Acad Sci USA 98:4190-4195
20. Fernandez-Cobo M, Gingalewski C, Drujan D, DeMaio A (1999) Downregulation of connexin 43 gene expression in rat heart during inflammation. The role of tumor necrosis factor. Cytokine 11:216-224
21. Findlay JBC (1990) Purification of membrane proteins. In: Harris ELV, Angal S (ed) Protein purification applications. IRL, Oxford, pp 59-82
22. Geimonen E, Jiang W, Ali M, Fishman GI, Gafield RE, Andersen J (1996) Activation of protein kinase C in human uterine smooth muscle induces connexin 43 gene transcription through an AP-1 site in the promotor sequence. J Biol Chem 271: 23667-23674
23. Goedert M, Cuenda A, Craxton M, Jakes R, Cohen P (1997) Activation of the novel stress-activated protein kinase SAPK4 by cytokines and cellular stresses is mediated by SKK3 (MKK6); comparison of its substrate specificity with that of other SAP kinases. EMBO J 16:3563-3571
24. Goh KC, Haque SJ, Williams BRG (1999) p38 MAP kinase is required for STAT1 serine phosphorylation and transcriptional activation induced by interferons. EMBO J 18:5601-5608
25. Gros DB, Jongsma HJ (1996) Connexins in mammalian heart function. Bioessays 18:719-730
26. Holmstroem TH, Tran SEF, Johnson VL, Ahn NG, Chow SC, Eriksson JE (1999) Inhibition of mitogen-activated kinase signaling sensitizes HeLa cells to Fas receptor-mediated apoptosis. Mol Cell Biol 19:5991-6002
27. Kanter HL, Laing JG, Beau SL, Beyer EC, Saffitz JE (1993a) Distinct patterns of connexin expression in canine Purkinje fibers and ventricular muscle. Circ Res 72:1124-1131
28. Kanter HL, Laing JG, Beyer EC, Green KG, Saffitz JE (1993b) Multiple connexins colocalize in canine ventricular myocyte gap junctions. Circ Res 73:344-350
29. Kwak BR, Jongsma HJ (1996) Regulation of cardiac gap junction channel permeability and conductance by several phosphorylating conditions. Mol Cell Biochem 157:93-99
30. Kwak BR, Van Veen TAB, Analbers LJS, Jongsma HJ (1995) TPA increases conductance but decreases permeability in neonatal rat cardiomyocyte gap junction channels. Exp Cell Res 220:456-463
31. Kyriakis JM, Avruch J (1996) Sounding the alarm: protein kinase cascades activated by stress and inflammation. J Biol Chem 271:24313-24316
32. Lau AF, Kurata WE, Kanemitsu MY et al (1996) Regulation of connexin 43 function by activated tyrosine protein kinases. J Bioenerg Biomembr 28:359-368
33. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193:265-275
34. McLaughlin MM, Kumar S, McDonnell PC, VanHorn S, Lee JC, Livi GP, Young PR (1996) Identification of mitogen-activated protein kinase-3, a novel substrate of CSBP p38 MAP kinase. J Biol Chem 271:8488-8492
35. Münster PN, Weingart R (1993) Effects of phorbol ester on gap junctions of neonatal rat heart cells. Pflügers Arch Eur J Physiol 423:181-188
36. Peters NS (1996) New insights into myocardial arrhythmogenesis: distribution of gap junctional coupling in normal, ischaemic and hypertrophied hearts. Clin Sci 90:447-452
37. Peters NS, Green CR, Poole-Wilson PA, Severs NJ (1993) Reduced content of connexin 43 gap junctions in ventricular myocardium from hypertrophied and ischemic human hearts. Circulation 88:864-875
38. Polontchouk L, Ebelt B, Jackels M, Dhein S (2002) Chronic effects of endothelin-1 and angiotensin-II on gap junctions and intercellular communication in cardiac cells. FASEB J 16:87-89 (online: Nov 14, 2001: PMID 11709493)
39. Sáez JC, Nairn AC, Czernick A, Spray DC, Hertzberg EL, Greengard P, Bennett MVL (1990) Phosphorylation of connexin 32, the hepatocyte gap junction protein, by cAMP-dependent protein kinase, protein kinase C and Ca/calmodulin-dependent protein kinase II. Eur J Biochem 192:263-273
40. Salameh A, Schomecker G, Breitkopf K, Dhein S, Klaus W (1996) The effect of the calcium-antagonist nitrendipine on intracellular calcium concentration in endothelial cells. Br J Pharmacol 118:1899-1904
41. Saxena M, Williams S, Brockdorff J, Gilman J, Mustelin T (1999a) Inhibition of T-cell signaling by mitogen-activated protein kinase-targeted hematopoietic tyrosine phosphatase (HePTP). J Biol Chem 274:11693-11700
42. Saxena M, Williams S, Taskén K, Mustelin T (1999b) Crosstalk between cAMP-dependent kinase and MAP kinase through a protein tyrosine phosphatase. Nat Cell Biol 1:305-310
43. Spray DC, Burt JM (1990) Structure-activity relations of the cardiac gap junction channel. Am J Physiol 258:C195-C205
44. Thomas TC, McNamee MG (1990) Purification of membrane proteins. In: Deutscher MP (ed) Guide to protein purification. Academic, San Diego, pp 499-507
45. Van Rijen HV, Van Kempen MV, Postma S, Jongsma HJ (1998) Tumor necrosis factor alpha alters the expression of connexin 43, connexin 40 and connexin 37 in human umbilical vein endothelial cells. Cytokine 10:258-264
46. Verheule S, Van Kempen MJA, Te Welscher PHJA, Kwak BR, Jongsma HJ (1997) Characterization of gap junction channels in adult rabbit atrial and ventricular myocardium. Circ Res 80:673-681
47. Warn-Cramer BJ, Cottrell GT, Burt JM, Lau AF (1998) Regulation of connexin-43 gap junctional intercellular communication by mitogen-activated protein kinase. J Biol Chem 273:9188-9196
48. Waskiewicz AJ, Cooper JA (1995) Mitogen and stress response pathways: MAP kinase cascades and phosphatase regulation in mammals and yeast. Curr Opin Cell Biol 7:798-805
49. Whitmarsh AJ, Davis RJ (1996) Transcription factor AP-1 regulation by mitogen-activated protein kinase signal transduction pathways. J Mol Med 74:589-607
50. Yang X, Chen Y, Gabuzda D (1999) ERK MAP kinase links cytokine signals to activation of latent HIV-1 infection by stimulating a cooperative interaction of AP-1 and NF-κB. J Biol Chem 274:27981-27988
51. 2-adrenergic receptor-induced p38 MAPK activation is mediated by protein kinase A rather than by Gi or Gβγ in adult mouse cardiomyocytes. J Biol Chem 275:40635-40640