Quantitative analysis of gene-specific DNA damage in human spermatozoa

Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

Volumn 529, Issue 1-2, 2003, Pages 21-34

  Sawyer, Dennis E ^a   Mercer, Belinda G ^a   Wiklendt, Agnieszka M ^a   Aitken, R John ^a,b  

^a UNIVERSITY OF NEWCASTLE   (Australia)

^b HUNTER MEDICAL RESEARCH INSTITUTE   (Australia)

Author keywords

DNA damage; Human spermatozoa; Mitochondrial DNA; Oxidative stress; Quantitative PCR

Indexed keywords

BETA GLOBIN; HYDROGEN PEROXIDE; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; IRON; MITOCHONDRIAL DNA;

ANIMAL CELL; ARTICLE; CELL IMMORTALIZATION; CONTROLLED STUDY; DNA DAMAGE; DNA ISOLATION; DNA STRAND BREAKAGE; GEL ELECTROPHORESIS; GENOTOXICITY; GERM CELL; HUMAN; HUMAN CELL; MALE; MOUSE; MYOBLAST; NONHUMAN; NORMAL HUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SPERMATOZOON; STATISTICAL SIGNIFICANCE;

EID: 0042009753     PISSN: 00275107     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0027-5107(03)00101-5     Document Type: Article

References (50)

1. Aitken R.J. The human spermatozoon - a cell in crisis? (The Amoroso Lecture). J. Reprod. Fertil. 115:1999;1-7.
2. Crow J.F. The origins. Nat. Rev. Genet. 1:2000;40-47.
3. Griveau J.F., Le Lannou D. Reactive oxygen species and human spermatozoa: physiology and pathology. Int. J. Androl. 20:1997;61-69.
4. Ames B.N., Shigenaga M.K., Hagen T.M. Oxidants, antioxidants, and the degenerative diseases of aging. Proc. Natl. Acad. Sci. U.S.A. 90:1993;7915-7922.
5. Fraga C.G., Motchnik P.A., Wyrobeck A.J., Rempel D.M., Ames B.N. Smoking and low antioxidant levels increase oxidative damage to sperm DNA. Mutat. Res. 351:1996;199-203.
6. Wells P.G., Kim P.M., Laposa R.R., Nicol C.J., Parman T., Winn L.M. Oxidative damage in chemical teratogenesis. Mutat. Res. 396:1997;65-78.
7. Ishihara M., Itoh M., Mayamoto K., Suna S., Takeuchi Y., Takenaka I., Jitsunari F. Spermatogenic disturbance induced by di-(2-ethylhexyl) phthalate is significantly prevented by treatment with antioxidant vitamins in the rat. Int. J. Androl. 23:2000;85-94.
8. Wellejus A., Poulsen H.E., Loft S. Iron-induced oxidative DNA damage in rat sperm cells in vivo and in vitro. Free Rad. Res. 32:2000;75-83.
9. Fraga C.G., Motchnik P.A., Shigenaga M.K., Helbock H.J., Jacob R.A., Ames B.N. Ascorbic acid protects against endogenous oxidative DNA damage in human sperm. Proc. Natl. Acad. Sci. U.S.A. 88:1991;11003-11006.
10. Oteiza P.I., Olin K.L., Fraga C.G., Keen C.L. Zinc deficiency causes oxidative damage to proteins, lipids and DNA in rat testes. J. Nutr. 125:1995;823-829.
11. Ochsendorf F.R. Infection and reactive oxygen species. Andrologia. 30(Suppl. 1):1998;81-86.
12. Comhaire F.H., Mahmoud A.M., Depuydt C.E., Zalata A.A., Christophe A.B. Mechanisms and effects of male genital tract infection on sperm quality and fertilizing potential: the andrologists viewpoint. Hum. Reprod. Update. 5:1999;393-398.
13. Hughes C.M., Lewis S.E., McKelvey-Martin V.J., Thompson W. A comparison of baseline and induced DNA damage in human spermatozoa from fertile and infertile men, using a modified comet assay. Mol. Hum. Reprod. 2:1996;613-619.
14. Aitken R.J., Gordon E., Harkiss D., Twigg J.P., Milne P., Jennings Z., Irvine D.S. Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa. Biol. Reprod. 59:1998;1037-1046.
15. Twigg J.P., Irvine D.S., Aitken R.J. Oxidative damage to DNA in human spermatozoa does not preclude pronucleus formation at intracytoplasmic sperm injection. Hum. Reprod. 13:1998;1864-1871.
16. Donnelly E.T., McClure N., Lewis S.E. The effect of ascorbate and α-tocopherol supplementation in vitro on DNA integrity and hydrogen peroxide-induced DNA damage in human spermatozoa. Mutagenesis. 14:1999;505-512.
17. Kemal D.N., Morshedi M., Oehninger S. Effects of hydrogen peroxide on DNA and plasma membrane integrity of human spermatozoa. Fertil. Steril. 74:2000;1200-1207.
18. Alvarez J.G., Touchstone J.C., Blasco L., Storey B.T. Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. J. Androl. 8:1987;338-348.
19. R.J. Aitken, J.S. Clarkson, Generation of reactive oxygen species by human spermatozoa, in: T. Dormandy, C. Rice-Evans (Eds.), Free Radicals: Recent Developments in Lipid Chemistry, Experimental Pathology and Medicine, Richelieu Press, London, 1987, pp. 333-335.
20. Aitken R.J., Clarkson J.S. Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J. Reprod. Fertil. 81:1987;459-469.
21. Aitken R.J., Clarkson J.S., Hargreave T.B., Irvine D.S., Wu F.C. Analysis of the relationship between defective sperm function and the generation of reactive oxygen species in cases of oligozoospermia. J. Androl. 10:1989;214-220.
22. Aitken R.J., Irvine D.S., Wu F.C. Prospective analysis of sperm-oocyte fusion and reactive oxygen species generation as criteria for the diagnosis of infertility. Am. J. Obstet. Gynecol. 164:1991;542-551.
23. D'Agata R., Vicari E., Moncada M.L., Sidoti G., Calogero A.E., Fornito M.C., Minacapilli G., Mongioi A., Polosa P. Generation of reactive oxygen species in subgroups of infertile men. Int. J. Androl. 13:1990;344-351.
24. Iwasaki A., Gagnon C. Formation of reactive oxygen species in spermatozoa of infertile patients. Fertil. Steril. 57:1992;409-416.
25. Pasqualotto F.F., Sharma R.K., Kobayashi H., Nelson D.R., Thomas A.J., Agarwal A. Oxidative stress in normospermic men undergoing infertility evaluation. J. Androl. 22:2001;316-322.
26. Sun J.G., Jurisicova A., Casper R.F. Detection of deoxyribonucleic acid fragmentation in human sperm: correlation with fertilization in vitro. Biol. Reprod. 56:1997;602-607.
27. Kodama H., Raizo Y., Fukuda J., Kasai H., Tanaka T. Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients. Fertil. Steril. 68:1997;519-524.
28. Lopes S., Sun J.G., Jurisicova A., Meriano J., Casper R.F. Sperm deoxyribonucleic acid fragmentation is increased in poor-quality semen samples and correlates with failed fertilization in intracytoplasmic sperm injection. Fertil. Steril. 69:1998;528-532.
29. Shen H.M., Chia S.E., Ong C.N. Evaluation of oxidative DNA damage in human sperm and its association with male infertility. J. Androl. 20:1999;718-723.
30. Host E., Lindenberg S., Smidt-Jensen S. DNA strand breaks in human spermatozoa: correlation with fertilization in vitro in oligozoospermic men and in men with unexplained infertility. Acta Obstet. Gynecol. Scand. 79:2000;189-193.
31. Irvine D.S., Twigg J.P., Gordon E.L., Fulton N., Milne P.A., Aitken R.J. DNA integrity in human spermatozoa: relationships with semen quality. J. Androl. 21:2000;33-44.
32. Aitken R.J., Clarkson J.S. Significance of reactive oxygen species and antioxidants in defining the efficacy of sperm preparation techniques. J. Androl. 9:1988;367-376.
33. J.D. Biggers, W.K. Whitten, D.G. Whittingham, The culture of mouse embryos in vitro, in: J.C. Daniel (Ed.), Methods in Mammalian Embryology, Freeman Press, San Francisco, 1971, pp. 86-94.
34. Hoffman M.C., Narisawa S., Hess R.A., Millan J.L. Immortalisation of germ cells and somatic testicular cells using the SV40 large T antigen. Exp. Cell Res. 201:1992;417-435.
35. Hoffman M.C., Hess R.A., Goldberg I., Millan J.L. Immortalised germ cells undergo meiosis in vitro. Proc. Natl. Acad. Sci. U.S.A. 91:1994;5533-5537.
36. Sawyer D.E., Roman S.D., Aitken R.J. Relative susceptibilities of mitochondrial and nuclear DNA damage induced by hydrogen peroxide in two mouse germ cell lines. Redox Rep. 6:2001;182-184.
37. Sutherland B.M., Bennett P.V., Sutherland J.C. DNA damage quantitation by alkaline gel electrophoresis. Methods Mol. Biol. 113:1999;183-202.
38. Yakes F.M., Van Houten B. Mitochondrial DNA damage is more extensive and persists longer than nuclear DNA damage in human cells following oxidative stress. Proc. Natl. Acad. Sci. U.S.A. 94:1997;514-519.
39. Ayala-Torres S., Chen Y., Svoboda T., Rosenblatt J., Van Houten B. Analysis of gene-specific DNA damage and repair using quantitative polymerase chain reaction. Methods. 22:2000;135-147.
40. F.M. Yakes, Y. Chen, B. Van Houten, PCR-based assays for the detection and quantitation of DNA damage and repair, in: G.P. Pfeifer (Ed.), Technologies for Detection of DNA Damage and Mutations, Plenum Press, New York and London, 1996, pp. 171-184.
41. Salazar J.J., Van Houten B. Preferential mitochondrial DNA injury caused by glucose oxidase as a steady generator of hydrogen peroxide in human fibroblasts. Mutat. Res. 385:1997;139-149.
42. Sawyer D.E., Van Houten B. Repair of DNA damage in mitochondria. Mutat. Res. 434:1999;161-176.
43. Laws G.M., Skopek T.R., Reddy M.V., Storer R.D., Glaab W.E. Detection of DNA adducts using a quantitative long PCR technique and the fluorogenic 5′ nuclease assay (Taqman). Mutat. Res. 484:2001;3-18.
44. Marnett L.J. Oxyradicals and DNA damage. Carcinogenesis. 21:2000;361-370.
45. Labat-Moleur F., Guillermet C., Lorimier P., Robert C., Lantuefoul S., Brambilla E., Negoescu A. TUNEL apoptotic cell detection in tissue sections: critical evaluation and improvement. J. Histochem. Cytochem. 46:1998;327-334.
46. Alvarez J.G., Storey B.T. Role of glutathione peroxidase in protecting mammalian spermatozoa from loss of motility caused by spontaneous lipid peroxidation. Gamete Res. 23:1989;77-90.
47. Armstrong J.S., Rajasekaran M., Chamulitrat W., Gatti P., Hellstrom W.J., Sikka S.C. Characterization of reactive oxygen species induced effects on human spermatozoa movement and energy metabolism. Free Rad. Biol. Med. 26:1999;869-880.
48. Ahmadi A., Ng S. Fertilizing ability of DNA-damaged spermatozoa. J. Exp. Zool. 284:1999;696-704.
49. Ahmadi A., Ng S. Developmental capacity of damaged spermatozoa. Hum. Reprod. 14:1999;2279-2285.
50. Imai H., Suzuki K., Ishizaka K., Ichinose S., Oshima H., Okayasu I., Emoto K., Umeda M., Nakagawa Y. Biol. Reprod. 64:2001;674-683.