Improving the transfection efficiency of post-mitotic neurons

Journal of Neuroscience Methods

Volumn 112, Issue 2, 2001, Pages 95-99

  Ohki, E C ^a   Tilkins, M L ^a   Ciccarone, V C ^b   Price, P J ^a  

^a Invitrogen Corporation   (United States)

^b Invitrogen Corporation   (United States)

Author keywords

Cationic lipids; Post mitotic cortical neurons; Post mitotic hippocampal neurons; Transfection

Indexed keywords

BETA GALACTOSIDASE; LIPOFECTAMINE; LIPOSOME;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BRAIN CORTEX; CELL VIABILITY; CYTOTOXICITY; EMBRYO; GENETIC TRANSFECTION; HIPPOCAMPUS; IMMUNOCYTOCHEMISTRY; INCUBATOR; MITOSIS; NONHUMAN; PRIORITY JOURNAL; RAT; REPORTER GENE; TEMPERATURE;

ANIMALS; BETA-GALACTOSIDASE; CATION EXCHANGE RESINS; CELLS, CULTURED; CENTRAL NERVOUS SYSTEM; CEREBRAL CORTEX; FETUS; GENES, REPORTER; GENETIC VECTORS; GLIAL FIBRILLARY ACIDIC PROTEIN; HIPPOCAMPUS; IMMUNOHISTOCHEMISTRY; INDICATORS AND REAGENTS; LIPIDS; NEURONS; PHOSPHOPYRUVATE HYDRATASE; RATS; TRANSFECTION;

EID: 0035892567     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(01)00441-1     Document Type: Article

References (6)

1. Serum-free B27/Neurobasal™ medium supports differentiated growth of neurons from the striatum, substantia nigra, septum, cerebral cortex, cerebellum and dentate gyrus
2. Viable cultured neurons in ambient carbon dioxide and hibernation storage for a month
3. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal™, a new serum-free medium combination
4. LIPOFECTAMINE 2000™ Reagent for rapid, efficient transfection of eukaryotic cells
5. Lipofection: a highly efficient, lipid mediated DNA transfection procedure
6. Endocytosis and intracellular processing accompanying transfection mediated by cationic liposomes