Lipid-mediated gene transfer into primary neurons using FuGene: Comparison to C6 glioma cells and primary glia

Experimental Neurology

Volumn 164, Issue 1, 2000, Pages 38-44

  Wiesenhofer, Bettina ^a   Humpel, Christian ^a  

^a UNIVERSITY HOSPITAL INNSBRUCK   (Austria)

Author keywords

In vitro; Liposomes; MAP 2; TH; galactosidase

Indexed keywords

BETA GALACTOSIDASE; DNA; LIPID; LIPOSOME; MICROTUBULE ASSOCIATED PROTEIN 2; TYROSINE 3 MONOOXYGENASE;

ANIMAL CELL; ARTICLE; CELL COUNT; CELL LABELING; CONTROLLED STUDY; DNA TRANSFER; DOPAMINERGIC NERVE CELL; EXPRESSION VECTOR; GENE TRANSFER; GENETIC TRANSFECTION; GLIA CELL; GLIOMA CELL; IMMUNOHISTOCHEMISTRY; NERVE CELL; NEWBORN; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REPORTER GENE;

EID: 0033940542     PISSN: 00144886     EISSN: None     Source Type: Journal    
DOI: 10.1006/exnr.2000.7414     Document Type: Article

References (18)

1. Choi-Lundberg D. L., Lin Q., Chang Y.-N., Chiang Y. L., Hay C. M., Mohajeri H., Davidson B. L., Bohn M. C. Dopaminergic neurons protected from degeneration by GDNF gene therapy. Science. 275:1997;838-841.
2. Cotten M., Baker A., Saltik M., Wagner E., Buschle M. Lipopolysaccharide is a frequent contaminant of plasmid DNA preparations and can be toxic to primary human cells in the presence of adenovirus. Gene Ther. 1:1994;239.
3. Felgner P. L., Gadek T. R., Holm M., Roman R., Chan H. W., Wenz M., Northrop J. P., Ringold G. M., Danielsen M. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc. Natl. Acad. Sci. USA. 84:1987;7413-7417.
4. Fisher L. J., Ray J. In vivo and ex vivo gene transfer to the brain. Curr. Opin. Neurobiol. 4:1994;735-741.
5. Hug P., Sleight R. G. Liposomes for the transformation of eukaryotic cells. Biochim. Biophys. Acta. 1097:1991;1-17.
6. Humpel C., Marksteiner J., Saria A. Glial-cell-line-derived neurotrophic factor enhances biosynthesis of substance P in striatal neurons in vitro. Cell Tissue Res. 286:1996;249-255.
7. Imaoka T., Date I., Miyoshi Y., Ono T., Furuta T., Asari S., Ohmoto T., Yasuda T., Tsuda M. Preliminary results of gene transfer to the central nervous system by continuous injection of DNA-liposome complex. Cell Transplant. 4:1995;S23-S26.
8. Imaoka T., Date I., Ohmoto T., Yasuda T., Tsuda M. In vivo gene transfer into the adult mammalian central nervous system by continuous injection of plasmid DNA-cationic liposome complex. Brain Res. 780:1998;119-128.
9. Kaech S., Kim J. B., Cariola M., Ralston E. Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons. Mol. Brain Res. 35:1996;344-348.
10. Kofler P., Wiesenhofer B., Rehrl C., Baier G., Stockhammer G., Humpel C. Liposome-mediated gene transfer into established CNS cell lines, primary glial cells, and in vivo. Cell Transplant. 7:1998;175-185.
11. Lapchak P. A., Araujo D. M., Hilt D. C., Sheng J., Jiao S. Adenoviral vector-mediated GDNF gene therapy in a rodent lesion model of late stage Parkinson's disease. Brain Res. 777:1997;153-160.
12. Lasic D. D., Papahadjopoulos D. Liposomes revisited. Science. 267:1995;1275-1276.
13. Loeffler J. P., Behr J. P. Gene transfer into primary and established mammalian cell lines with lipopolyamine-coated DNA. Methods Enzymol. 217:1993;599-619.
14. Murray E. J. Gene Transfer and Expression Protocols. 1991;Humana Press, Clifton.
15. Ridet J. L., Privat A. Gene therapy in the central nervous system: Direct versus indirect gene delivery. J. Neurosci. Res. 42:1995;287-293.
16. White D., Butler B., Creswell D., Smith C. Wizard PureFection plasmid DNA purification system: The new standard in isolating transfection grade plasmid DNA. Promega Notes. 68:1998;2-6.
17. Wiesenhofer B., Kaufmann W. A., Humpel C. Improved lipid-mediated gene transfer in C6 glioma cells and primary glial cells using FuGene. J. Neurosci. Methods. 92:1999;145-152.
18. Yang K., Faustinella F., Xue J. J., Whitson J., Kampfl A., Mu X. S., Zhao X., Taglialatela G., Perez-Polo J. R., Clifton G., Hayes R. L. Optimizing liposome-mediated gene transfer in primary rat septo-hippocampal cell cultures. Neurosci. Lett. 182:1994;287-290.