메뉴 건너뛰기




Volumn 28, Issue 2, 2003, Pages 362-367

A Rubredoxin based system for screening of protein expression conditions and on-line monitoring of the purification process

Author keywords

[No Author keywords available]

Indexed keywords

THERMOTOGA MARITIMA;

EID: 0038395853     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1046-5928(02)00704-0     Document Type: Article
Times cited : (12)

References (19)
  • 1
    • 0036145552 scopus 로고    scopus 로고
    • Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli
    • M. Hammarstrom, N. Hellgren, S. van Den Berg, H. Berglund, T. Hard, Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli, Protein Sci. 11 (2002) 313-321.
    • (2002) Protein Sci , vol.11 , pp. 313-321
    • Hammarstrom, M.1    Hellgren, N.2    Van Den Berg, S.3    Berglund, H.4    Hard, T.5
  • 2
    • 0026681502 scopus 로고
    • Cloning, sequencing and expression in Escherichia coli of the rubredoxin gene from Clostridium pasteurianum
    • I. Mathieu, J. Meyer, J.M. Moulis, Cloning, sequencing and expression in Escherichia coli of the rubredoxin gene from Clostridium pasteurianum, Biochem. J. 285 (Pt 1) (1992) 255-262.
    • (1992) Biochem J. , vol.285 , Issue.PART 1 , pp. 255-262
    • Mathieu, I.1    Meyer, J.2    Moulis, J.M.3
  • 4
    • 0033814033 scopus 로고    scopus 로고
    • Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins
    • A. Skerra, T.G. Schmidt, Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins, Methods Enzymol. 326 (2000) 271-304.
    • (2000) Methods Enzymol. , vol.326 , pp. 271-304
    • Skerra, A.1    Schmidt, T.G.2
  • 5
    • 0023806075 scopus 로고
    • Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase
    • D.B. Smith, K.S. Johnson, Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase, Gene 67 (1988) 31-40.
    • (1988) Gene , vol.67 , pp. 31-40
    • Smith, D.B.1    Johnson, K.S.2
  • 7
    • 0031828101 scopus 로고    scopus 로고
    • Sticky-end PCR: New method for subcloning
    • G. Zeng, Sticky-end PCR: new method for subcloning, Biotechniques 25 (1998) 206-208.
    • (1998) Biotechniques , vol.25 , pp. 206-208
    • Zeng, G.1
  • 8
    • 0034947940 scopus 로고    scopus 로고
    • Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase
    • see also p. 92
    • M. Geiser, R. Cebe, D. Drewello, R. Schmitz, Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase, Biotechniques 31 (2001) 88-90, see also p. 92.
    • (2001) Biotechniques , vol.31 , pp. 88-90
    • Geiser, M.1    Cebe, R.2    Drewello, D.3    Schmitz, R.4
  • 11
    • 0033673137 scopus 로고    scopus 로고
    • High yield of Methylophilus methylotrophus cytochrome c by coexpression with cytochrome c maturation gene cluster from Escherichia coli
    • N.J. Price, L. Brennan, T.Q. Faria, E. Vijgenboom, G.W. Canters, D.L. Turner, H. Santos, High yield of Methylophilus methylotrophus cytochrome c by coexpression with cytochrome c maturation gene cluster from Escherichia coli, Protein Expr. Purif. 20 (2000) 444-450.
    • (2000) Protein Expr. Purif. , vol.20 , pp. 444-450
    • Price, N.J.1    Brennan, L.2    Faria, T.Q.3    Vijgenboom, E.4    Canters, G.W.5    Turner, D.L.6    Santos, H.7
  • 12
    • 0035845538 scopus 로고    scopus 로고
    • Biosynthesis of a fluorescent cyanobacterial C-phycocyanin holo-alpha subunit in a heterologous host
    • A.J. Tooley, Y.A. Cai, A.N. Glazer, Biosynthesis of a fluorescent cyanobacterial C-phycocyanin holo-alpha subunit in a heterologous host, Proc. Natl. Acad. Sci. USA 98 (2001) 10560-10565.
    • (2001) Proc. Natl. Acad. Sci. USA , vol.98 , pp. 10560-10565
    • Tooley, A.J.1    Cai, Y.A.2    Glazer, A.N.3
  • 13
    • 0035128545 scopus 로고    scopus 로고
    • Purification of recombinant green fluorescent protein using chromatofocusing with a pH gradient composed of multiple stepwise fronts
    • C.R. Narahari, L. Randers-Eichhorn, J.C. Strong, N. Ramasubramanyan, G. Rao, D.D. Frey, Purification of recombinant green fluorescent protein using chromatofocusing with a pH gradient composed of multiple stepwise fronts, Biotechnol. Prog. 17 (2001) 150-160.
    • (2001) Biotechnol. Prog. , vol.17 , pp. 150-160
    • Narahari, C.R.1    Randers-Eichhorn, L.2    Strong, J.C.3    Ramasubramanyan, N.4    Rao, G.5    Frey, D.D.6
  • 14
    • 0034682666 scopus 로고    scopus 로고
    • EGFP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy
    • S. Jakobs, V. Subramaniam, A. Schonle, T.M. Jovin, S.W. Hell, EGFP and DsRed expressing cultures of Escherichia coli imaged by confocal, two-photon and fluorescence lifetime microscopy, FEBS Lett. 479 (2000) 131-135.
    • (2000) FEBS Lett. , vol.479 , pp. 131-135
    • Jakobs, S.1    Subramaniam, V.2    Schonle, A.3    Jovin, T.M.4    Hell, S.W.5
  • 15
    • 0034888061 scopus 로고    scopus 로고
    • Overexpression and purification of Helicobacter pylori flavodoxin and induction of a specific antiserum in rabbits
    • R. Paul, F.U. Bosch, K.P. Schafer, Overexpression and purification of Helicobacter pylori flavodoxin and induction of a specific antiserum in rabbits, Protein Expr. Purif. 22 (2001) 399-405.
    • (2001) Protein Expr. Purif. , vol.22 , pp. 399-405
    • Paul, R.1    Bosch, F.U.2    Schafer, K.P.3
  • 16
    • 0034490961 scopus 로고    scopus 로고
    • Thermal stability of Clostridium pasteurianum rubredoxin: Deconvoluting the contributions of the metal site and the protein
    • F. Bonomi, D. Fessas, S. Iametti, D.M. Kurtz, S. Mazzini, Thermal stability of Clostridium pasteurianum rubredoxin: deconvoluting the contributions of the metal site and the protein, Protein Sci. 9 (2000) 2413-2426.
    • (2000) Protein Sci. , vol.9 , pp. 2413-2426
    • Bonomi, F.1    Fessas, D.2    Iametti, S.3    Kurtz, D.M.4    Mazzini, S.5
  • 17
    • 0036489443 scopus 로고    scopus 로고
    • Green fluorescent protein (GFP): Applications, structure, and related photophysical behavior
    • M. Zimmer, Green fluorescent protein (GFP): applications, structure, and related photophysical behavior, Chem. Rev. 102 (2002) 759-781.
    • (2002) Chem. Rev. , vol.102 , pp. 759-781
    • Zimmer, M.1
  • 19
    • 0027297726 scopus 로고
    • Reversed-phase capillary high-performance liquid chromatography with on-line UV, fluorescence and electrospray ionization mass spectrometric detection in the analysis of peptides and proteins
    • T.G. Heath, A.B. Giordani, Reversed-phase capillary high-performance liquid chromatography with on-line UV, fluorescence and electrospray ionization mass spectrometric detection in the analysis of peptides and proteins, J. Chromatogr. A 638 (1993) 9-19.
    • (1993) J. Chromatogr. A , vol.638 , pp. 9-19
    • Heath, T.G.1    Giordani, A.B.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.