High yield of Methylophilus methylotrophus cytochrome c'' by coexpression with cytochrome c maturation gene cluster from Escherichia coli

Protein Expression and Purification

Volumn 20, Issue 3, 2000, Pages 444-450

  Price, Nicholas J ^a   Brennan, Lorraine ^a   Faria, Tiago Q ^a   Vijgenboom, Erik ^b   Canters, Gerard W ^b   Turner, David L ^c   Santos, Helena ^a  

^a INSTITUTO DE TECNOLOGIA QUÍMICA E BIOLÓGICA   (Portugal)

^b LEIDEN UNIVERSITY   (Netherlands)

^c UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AEROBIC CELL CULTURE; ANALYTIC METHOD; CYTOCHROME C; ESCHERICHIA COLI; GENE CLUSTER; GENE EXPRESSION; GENETIC ENGINEERING; METHYLOPHILUS METHYLOTROPHUS; TARGETED MUTAGENESIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; METHYLOPHILUS METHYLOTROPHUS;

EID: 0033673137     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1318     Document Type: Article

References (25)

1. Characterization and NMR studies of a novel cytochrome c isolated from Methylophilus methylotrophus which shows a redox-linked change of spin-state
2. 3+ haem with near-perpendicular orientation of the ligands
3. Involvement of a labile axial histidine in coupling electron and proton transfer in Methylophilus methylotrophus cytochrome c''
4. 13C NMR
5. 1H-NMR
6. 3 from Desulfovibrio vulgaris [Hildenborough] in Escherichia coli: Export and processing of the apoprotein
7. Expression in Escherichia coli of c-type cytochrome genes from Rhodopseudomonas viridis
8. 2 in Escherichia coli
9. 550 can be synthesised in aerobic Escherichia coli
10. 552 gene from Hydrogenobacter thermophilus
11. 550 from Thiobacillus versutus: Cloning, expression in Escherichia coli, and purification of the heterologous holoprotein
12. 6 from Synechocystis 6803
13. 552 does not
14. Bacterial expression of a mitochondrial cytochrome c. Trimethylation of Lys72 in Yeast iso-1-cytochrome c and the alkaline conformational transition
15. 6 from the cyanobacterium Anabaena sp. PCC 7119
16. 552 acting as electron donor to the Paracoccus denitrificans cytochrome c oxidase
17. 3 oxidase in Escherichia coli
18. Escherichia coli genes required for cytochrome c maturation
19. Cloning and sequence analysis of the gene encoding Methylophilus methylotrophus cytochrome c'', a unique protein with a perpendicular orientation of the histidinyl ligands
20. The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts
21. A two-dimensional nuclear Overhauser enhancement [2D NOE] experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromolecules
22. Gradient-tailored excitation for single quantum NMR spectroscopy of aqueous solutions
23. Suppression of cross relaxation in TOCSY experiments on macromolecules
24. 1H spin system identification in macromolecules