Engineering a "methionine clamp" into Src family kinases enhances specificity toward unnatural ATP analogues

Biochemistry

Volumn 42, Issue 26, 2003, Pages 7915-7921

  Ulrich, Scott M ^b   Kenski, Denise M ^a   Shokat, Kevan M ^b,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CELLS; ENZYMES;

RADIOLABELING;

BIOCHEMISTRY;

6 N BENZYLADENOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE; GLYCINE; METHIONINE; NUCLEOTIDE; PROTEIN TYROSINE KINASE; UNCLASSIFIED DRUG;

ALLELE; ANIMAL CELL; ARTICLE; BINDING SITE; CATALYSIS; CELL LYSATE; CONCENTRATION (PARAMETERS); ENZYME SPECIFICITY; ISOTOPE LABELING; MOUSE; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; SIGNAL NOISE RATIO;

3T3 CELLS; ADENOSINE TRIPHOSPHATE; ANIMALS; BINDING SITES; IMMUNOBLOTTING; KINETICS; METHIONINE; MICE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; ONCOGENE PROTEIN PP60(V-SRC); PHOSPHORYLATION; PHOSPHOTYROSINE; PROTEIN ENGINEERING; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-FYN; RECOMBINANT FUSION PROTEINS; SUBSTRATE SPECIFICITY;

ANIMALIA;

EID: 0038047895     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi030042a     Document Type: Article

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