Flow cytometric measurement of phosphorylated STAT5 in AML: Lack of specific association with FLT3 internal tandem duplications

Leukemia Research

Volumn 27, Issue 9, 2003, Pages 803-805

  Pallis, Monica ^a   Seedhouse, Claire ^a   Grundy, Martin ^a   Russell, Nigel ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

AML; FLT3 ITD; Phosphorylated STAT5

Indexed keywords

BCR ABL PROTEIN; ENZYME INHIBITOR; FLT3 LIGAND; IMATINIB; PHOSPHOPROTEIN; PROTEIN TYROSINE KINASE; STAT5 PROTEIN;

ACUTE GRANULOCYTIC LEUKEMIA; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; ENZYME ACTIVITY; ENZYME INHIBITION; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; QUANTITATIVE ANALYSIS; TANDEM REPEAT; VALIDATION PROCESS;

EID: 0037865499     PISSN: 01452126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0145-2126(03)00012-2     Document Type: Article

References (14)

1. Coffer P.J., Koenderman L., de Groot R.P. The role of STATs in myeloid differentiation and leukemia. Oncogene. 19:2000;2511-2522.
2. Mui A.L., Wakao H., O'Farrell A.M., Harada N., Miyajima A. Interleukin-3, granulocyte-macrophage colony stimulating factor and interleukin-5 transduce signals through two STAT5 homologs. EMBO J. 14:1995;1166-1175.
3. Zhang S., Fukuda S., Lee Y., Hangoc G., Cooper S., Spolski R.et al. Essential role of signal transducer and activator of transcription (Stat)5a but not Stat5b for Flt3-dependent signaling. J. Exp. Med. 192:2000;719-728.
4. Weber-Nordt R.M., Egen C., Wehinger J., Ludwig W., Gouilleux-Gruart V., Mertelsmann R.et al. Constitutive activation of STAT proteins in primary lymphoid and myeloid leukemia cells and in Epstein-Barr virus (EBV)-related lymphoma cell lines. Blood. 88:1996;809-816.
5. Xia Z., Baer M.R., Block A.W., Baumann H., Wetzler M. Expression of signal transducers and activators of transcription proteins in acute myeloid leukemia blasts. Cancer Res. 58:1998;3173-3180.
6. Birkenkamp K.U., Geugien M., Lemmink H.H., Kruijer W., Vellenga E. Regulation of constitutive STAT5 phosphorylation in acute myeloid leukemia blasts. Leukemia. 15:2001;1923-1931.
7. Spiekermann K., Pau M., Schwab R., Schmieja K., Franzrahe S., Hiddemann W. Constitutive activation of STAT3 and STAT5 is induced by leukemic fusion proteins with protein tyrosine kinase activity and is sufficient for transformation of hematopoietic precursor cells. Exp. Hematol. 30:2002;262-271.
8. Kiyoi H., Naoe T., Nakano Y., Yokota S., Minami S., Miyawaki S.et al. Prognostic implication of FLT3 and N-RAS gene mutations in acute myeloid leukemia. Blood. 93:1999;3074-3080.
9. Kottaridis P.D., Gale R.E., Frew M.E., Harrison G., Langabeer S.E., Belton A.A.et al. The presence of a FLT3 internal tandem duplication in patients with acute myeloid leukemia (AML) adds important prognostic information to cytogenetic risk group and response to the first cycle of chemotherapy: analysis of 854 patients from the United Kingdom Medical Research Council AML 10 and 12 trials. Blood. 98:2001;1752-1759.
10. Mizuki M., Fenski R., Halfter H., Matsumura I., Schmidt R., Muller C.et al. Flt3 mutations from patients with acute myeloid leukemia induce transformation of 32D cells mediated by the Ras and STAT5 pathways. Blood. 96:2000;3907-3914.
11. Hayakawa F., Towatari M., Kiyoi H., Tanimoto M., Kitamura T., Saito H.et al. Tandem-duplicated Flt3 constitutively activates STAT5 and MAP kinase and introduces autonomous cell growth in IL-3-dependent cell lines. Oncogene. 19:2000;624-631.
12. Fleisher T.A., Dorman S.E., Anderson J.A., Vail M., Brown M.R., Holland S.M. Detection of intracellular phosphorylated STAT-1 by flow cytometry. Clin. Immunol. 90:1999;425-430.
13. Carlesso N., Frank D.A., Griffin J.D. Tyrosyl phosphorylation and DNA binding activity of signal transducers and activators of transcription (STAT) proteins in hematopoietic cell lines transformed by Bcr/Abl. J. Exp. Med. 183:1996;811-820.
14. Donato N.J., Wu J.Y., Zhang L., Kantarjian H., Talpaz M. Down-regulation of interleukin-3/granulocyte-macrophage colony-stimulating factor receptor beta-chain in BCR-ABL(+) human leukemic cells: association with loss of cytokine-mediated Stat-5 activation and protection from apoptosis after BCR-ABL inhibition. Blood. 97:2001;2846-2853.