A modular PIP2 binding site as a determinant of capsaicin receptor sensitivity

Science

Volumn 300, Issue 5623, 2003, Pages 1284-1288

  Prescott, Elizabeth D ^a   Julius, David ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVATION ANALYSIS; HYDROLYSIS; LIPIDS; PATHOLOGY; PHOSPHATES; PHYSIOLOGY; POTASSIUM;

PATHOPHYSIOLOGICAL CONDITIONS;

MOLECULAR BIOLOGY;

ION CHANNEL; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE; PHOSPHOLIPASE C; POTASSIUM CHANNEL; VANILLOID RECEPTOR; ARSENOSOBENZENE; CAPSAICIN; CARRIER PROTEIN; DRUG RECEPTOR; EPIDERMAL GROWTH FACTOR RECEPTOR; HYBRID PROTEIN; INWARDLY RECTIFYING POTASSIUM CHANNEL; MEMBRANE PROTEIN; ORGANOARSENIC DERIVATIVE; PROTEIN TYROSINE KINASE A;

NEUROLOGY;

ANIMAL CELL; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CHANNEL GATING; CONTROLLED STUDY; ENZYME ACTIVATION; FEMALE; GENE MUTATION; HEAT; NONHUMAN; PAIN; PATHOPHYSIOLOGY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN HYDROLYSIS; PROTEIN LIPID INTERACTION; PROTEIN PROTEIN INTERACTION; RECEPTOR SENSITIVITY; SENSORY NERVE CELL; THERMAL STIMULATION; XENOPUS; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; GENE DELETION; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; MUTATION; OOCYTE; PATCH CLAMP; PHOSPHORYLATION; PROTEIN TERTIARY STRUCTURE; RAT;

ANIMALIA;

AMINO ACID SEQUENCE; ANIMALS; ARSENICALS; BINDING SITES; CAPSAICIN; CARRIER PROTEINS; HEAT; HUMANS; ION CHANNEL GATING; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MUTATION; OOCYTES; PATCH-CLAMP TECHNIQUES; PHOSPHATIDYLINOSITOL 4,5-DIPHOSPHATE; PHOSPHOLIPASE C; PHOSPHORYLATION; POTASSIUM CHANNELS, INWARDLY RECTIFYING; PROTEIN STRUCTURE, TERTIARY; RATS; RECEPTOR, EPIDERMAL GROWTH FACTOR; RECEPTOR, TRKA; RECEPTORS, DRUG; RECOMBINANT FUSION PROTEINS; SEQUENCE DELETION; XENOPUS;

EID: 0037799198     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1083646     Document Type: Article

References (25)

1. D. W. Hilgemann, S. Feng, C. Nasuhoglu, Sci. STKE 2001, RE19 (2001).
2. R. C. Hardie, Annu. Rev. Physiol. 65, 735 (2003).
3. H. H. Chuang et al., Nature 411, 957 (2001).
4. M. Tominaga, M. Wada, M. Masu, Proc. Natl. Acad. Sci. U.S.A. 98, 6951 (2001).
5. L. W. Runnels, L. Yue, D. E. Clapham, Nature Cell Biol. 4, 329 (2002).
6. M. J. Caterina et al., Nature 389, 816 (1997).
7. M. Tominaga et al., Neuron 21, 531 (1998).
8. M. J. Caterina et al., Science 288, 306 (2000).
9. J. B. Davis et al., Nature 405, 183 (2000).
10. E. D. Prescott, D. Julius, unpublished data.
11. H. Zhang, C. He, X. Yan, T. Mirshahi, D. E. Logothetis, Nature Cell Biol. 1, 183 (1999).
12. C. L. Huang, S. Feng, D. W. Hilgemann, Nature 391, 803 (1998).
13. E. Kobrinsky, T. Mirshahi, H. Zhang, T. Jin, D. E. Logothetis, Nature Cell Biol. 2, 507 (2000).
14. C. Wiedemann, T. Schafer, M. M. Burger, EMBO J. 15, 2094 (1996).
15. V. Vellani, S. Mapplebeck, A. Moriondo, J. B. Davis, P. A. McNaughton, J. Physiol. (London) 534, 813 (2001).
16. L. S. Premkumar, G. P. Ahem, Nature 408, 985 (2000).
17. M. Numazaki, T. Tominaga, H. Toyooka, M. Tominaga, J. Biol. Chem. 277, 13375 (2002).
18. P. M. Zygmunt et al., Nature 400, 452 (1999).
19. T. Hofmann et al., Nature 397, 259 (1999).
20. S. E. Jordt, D. Julius, Cell 108, 421 (2002).
21. A. M. Peier et al., Science 296, 2046 (2002).
22. G. D. Smith et al., Nature 418, 186 (2002).
23. H. Xu et al., Nature 418, 181 (2002).
24. Materials and methods, fig. S1, and associated references are available as supporting material an Science Online.
25. We thank R. Nicoll and D. Minor for many helpful suggestions and encouragement throughout this project, G. Reid for help with the Peltier system, and members of our laboratory for advice. This work was supported by predoctoral fellowships from the NSF and the University of California, San Francisco, Chancellor's Fund (E.D.P.) and by the NIH (D.J.).