Soluble human core 2 β6-N-acetylglucosaminyltransferase C2GnT1 requires its conserved cysteine residues for full activity

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1648, Issue 1-2, 2003, Pages 62-74

  Yang, Xiaojing ^a   Qin, Wensheng ^a,b   Lehotay, Michael ^a   Toki, Dale ^c   Dennis, Philip ^b   Schutzbach, John S ^a   Brockhausen, Inka ^a,b,c  

^a QUEEN S UNIVERSITY   (Canada)

^b UNIVERSITY HEALTH NETWORK   (Canada)

^c HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

Core 2; Cysteine; GlcNAc transferase; Mutant; O glycan branching

Indexed keywords

2 BETA6 N ACETYLGLUCOSAMINYLTRANSFERASE; 5,5' DITHIOBIS(2 NITROBENZOIC ACID); CYSTEINE; DITHIOTHREITOL; MERCAPTOETHANOL; N ETHYLMALEIMIDE; SERINE; THIOL DERIVATIVE; TRANSFERASE; UNCLASSIFIED DRUG; BETA 1,3 GALACTOSYL O GLYCOSYL GLYCOPROTEIN BETA 1,6 ACETYLGLUCOSAMINYL TRANSFERASE; BETA-1,3-GALACTOSYL-O-GLYCOSYL-GLYCOPROTEIN BETA-1,6-ACETYLGLUCOSAMINYL TRANSFERASE; N ACETYLGLUCOSAMINYLTRANSFERASE; REDUCING AGENT;

ARTICLE; CONCENTRATION (PARAMETERS); DISULFIDE BOND; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INACTIVATION; ENZYME ISOLATION; ENZYME STABILITY; INSECT CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; WILD TYPE; AMINO ACID SEQUENCE; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; SEQUENCE ALIGNMENT; STRUCTURE ACTIVITY RELATION;

INSECTA;

AMINO ACID SEQUENCE; CONSERVED SEQUENCE; CYSTEINE; HUMANS; MOLECULAR SEQUENCE DATA; MUTATION; N-ACETYLGLUCOSAMINYLTRANSFERASES; REDUCING AGENTS; SEQUENCE ALIGNMENT; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0037629966     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1570-9639(03)00105-5     Document Type: Article

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