Effect of salts on lysozyme stability at the water-oil interface and upon encapsulation in poly(lactic-co-glycolic) acid microspheres

Biotechnology and Bioengineering

Volumn 82, Issue 7, 2003, Pages 825-832

  Pérez, Caroline ^a   Griebenow, Kai ^a  

^a UNIVERSITY OF PUERTO RICO   (Puerto Rico)

Author keywords

Lysozymes; Protein encapsulation; Protein stability; Salt effects; Water in oil in water technique

Indexed keywords

AGGLOMERATION; EMULSIONS; PRECIPITATION (CHEMICAL); SALTS;

ELECTROSELECTIVITY;

PROTEINS;

ANION; DICHLOROMETHANE; EGG PROTEIN; EGG WHITE; INORGANIC SALT; LYSOZYME; MICROSPHERE; OIL; POLYGLACTIN; POTASSIUM DIHYDROGEN PHOSPHATE; THIOCYANATE POTASSIUM; WATER;

AQUEOUS SOLUTION; ARTICLE; EMULSION; ENCAPSULATION; ENZYME ACTIVITY; ENZYME INACTIVATION; PROTEIN AGGREGATION; PROTEIN DEGRADATION; PROTEIN STABILITY; TECHNIQUE;

ANIMALS; CHICKENS; COATED MATERIALS, BIOCOMPATIBLE; DELAYED-ACTION PREPARATIONS; DRUG STABILITY; EGG PROTEINS; EMULSIONS; ENZYME ACTIVATION; LACTIC ACID; MICROSPHERES; MURAMIDASE; OILS; POLYGLYCOLIC ACID; POLYMERS; SALTS; SURFACE PROPERTIES; TEMPERATURE; WATER;

EID: 0037572320     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10632     Document Type: Article

References (36)

1. Alonso MJ, Gupta RK, Min C, Siber GR, Langer R. 1994. Biodegradable microspheres as controlled-release tetanus toxoid delivery systems. Vaccine 12:299-306.
2. Blanco D, Alonso MJ. 1998. Protein encapsulation and release from poly (lactide-co-glycolide) microspheres: effect of the protein and polymer properties and of the co-encapsulation of surfactants. Eur J Pharm Biopharm 45:285-294.
3. Carrasquillo KG, Sanchez C, Griebenow K. 2000. Relationship between conformational stability and lyophilization-induced structural changes in chymotrypsin. Biotechnol Appl Biochem 31:41-53.
4. Cleland JL, Jones AJS. 1996. Stable formulations of recombinant human growth hormone and interferon-γ for microencapsulation in biodegradable microspheres. Pharm Res 13:1464-1475.
5. Curtis RA, Prausnitz JM, Blanch HW. 1998. Protein-protein and protein-salt interactions in aqueous protein solutions containing concentrated electrolytes. Biotechnol Bioeng 57:11-21.
6. Curtis RA, Ulrich J, Montaser A, Prausnitz JM, Blanch HW. 2002. Protein-protein interactions in concentrated electrolyte solutions. Biotechnol Bioeng 79:367-380.
7. Eggers DK, Valentine JS. 2001. Molecular confinement influences protein structure and enhances thermal protein stability. Protein Sci 10:250-261.
8. Fan YX, Ju M, Zhou JM, Tsou CL. 1995. Activation of chicken liver dihydropholate reductase in concentrated urea solutions. Biochim Biophys Acta 1257:151-157.
9. Ghaderi R, Carlfors J. 1997. Biological activity of lysozyme after entrapment in poly(D,L-lactide-co-glycolide)-microspheres. Pharm Res 14:1556-1564.
10. Goto Y, Takahashi N, Fink AL. 1990. Mechanism of acid-induced folding of proteins. Biochemistry 29:3480-3488.
11. Hamaguchi K, Sakai H. 1965. Structure of lysozyme. IX. The effect of temperature on the conformation of lysozyme. J Biochem 57:721-732.
12. Huang X, Knoell CT, Timasheff SN. 2001. Modulation of recombinant human prostate-specific antigen: Activation by Hofmeister salts and inhibition by azapeptides. Biochemistry 40:11734-11741.
13. Imoto T, Leslie SF, Rupley JA, Tanaka F. 1971. Fluorescence of lysozyme: emissions from tryptophan residues 62 and 108 and energy migration. Proc Nat Acad Sci USA 69:1151-1155.
14. Langer R. 1993. Polymer-controlled drug delivery systems. Acc Chem Res 26:537-542.
15. Leo E, Pecquet S, Rojas J, Couvreur P, Fattal E. 1998. Changing the pH of the external aqueous phase may modulate protein entrapment and delivery from poly(lactide-co-glycolide) microspheres prepared by a w/o/w solvent evaporation method. J Microencapsul 15:421-430.
16. Lu W, Park TG. 1995b. Protein release from poly(lactic-co-glycolic acid) microspheres: Protein stability problems. PDA J Pharm Sci Technol 49:13-19.
17. Malzert A, Boury F, Renard D, Robert P, Proust JE, Benoît JP. 2002. Influence of some formulation parameters on lysozyme adsorption and on its stability in solution. Int J Pharm 242:405-409.
18. McGee, JP, Singh M, Li XM, Qiu H, O'Hagan DT. 1997. The encapsulation of a model protein in poly(D,L lactide-co-glycolide) microparticles of various sizes: an evaluation of process reproducibility. J Microencapsul 14:197-210.
19. Morlock M, Kissel T, Li YX, Koll H, Winter GJ. 1997. Microencapsulation of rh-erythropoietin, using biodegradable poly(D,L-lactide-coglycolide): protein stability and the effects of stabilizing excipients. J Controlled Rel 56:105-115.
20. Neagu A, Neagu M, Der A. 2001. Fluctuations and the Hofmeister effect. Biophys J 81:1285-1294.
21. Nemes C, Rozlosnik N, Ramsdem JJ. 1999. Direct measurements of the viscoelasticity of adsorbed proteins layers using atomic force microscopy. Phys Rev E 60:1166-1169.
22. Nihant N, Schugens C, Grandfils C, Jérôme CR, Teyssié P. 1994. Polylactide microparticles prepared by double emulsion/evaporation technique. I. Effect of primary emulsion stability. Pharm Res 11:1479-1484.
23. Pérez C, Griebenow K. 2000. Fourier-transform infrared spectroscopic investigation of the thermal denaturation of hen egg-white lysozyme dissolved in aqueous buffer and glycerol. Biotechnol Lett 22:1899-1905.
24. Pérez C, Griebenow K. 2001. Improved activity and stability of lysozyme at the water/CH2CL2 interface: enzyme unfolding and aggregation and its prevention by polyols. J Pharm Pharmacol 53:1217-1226.
25. Pérez C, Castellanos IJ,Costantino HR, Al-Azzam W, Griebenow K. 2002a. Recent trends in stabilizing protein structure upon encapsulation and release from bioerodible polymers. J Pharm Pharmacol 54:301-313.
26. Pérez C, De Jesus P, Griebenow K. 2002b. Preservation of lysozyme structure and function upon encapsulation and release from poly(lactic-co-glycolic) acid microspheres prepared by the water-in-oil-in-water-method. Int J Pharm 248:193-206.
27. Pistel KF, Kissel T. 2000. Effects of salt addition on the microencapsulation of proteins using w/o/w double emulsion technique. J Microencapsul 17:467-483.
28. Sah H. 1999a. Protein instability toward organic solvent/water emulsification: implications for protein microencapsulation into microspheres. PDA J Pharm Sci Technol 53:3-10.
29. Sah H. 1999b. Stabilization of proteins against methylene chloride/water interface-induced denaturation and aggregation. J Controlled Rel 58:143-151.
30. Sah H. 1999c. Protein behavior at the water/methylene chloride interface. J Pharm Sci 88:1320-1325.
31. Sah H, Choi SK, Choi HG, Yong CS. 2002. Relation of dynamic changes in interfacial tension to protein destabilization upon emulsification. Arch Pharm Res 25:381-386.
32. Timasheff SN. 1998. Control of protein stability and reactions by weakly interacting cosolvents: the simplicity of the complicated. Adv Protein Chem 51:355-432.
33. Tobler SA, Sherman NE, Fernandez EJ. 2001. Tracking lysozyme unfolding during salt-induced precipitation with hydrogen exchange and mass spectrometry. Biotechnol Bioeng 71:194-207.
34. Tsou C-L. 1993. Conformational flexibility of enzyme active sites. Science 262:380-381.
35. Tsou C-L. 1998. The role of active site flexibility in enzyme catalysis. Biochemistry (Moscow) 63:253-258.
36. Van de Weert M, Hoechstetter J, Hennink WE, Crommelin D.J.A. 2000. The effect of a water/organic solvent interface on the structural stability of lysozyme. J Controlled Rel 68:31-40.