Interference with the cytoplasmic tail of gp210 disrupts "close apposition" of nuclear membranes and blocks nuclear pore dilation

Journal of Cell Biology

Volumn 158, Issue 1, 2002, Pages 53-62

  Drummond, Sheona P ^a,b   Wilson, Katherine L ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b CHRISTIE HOSPITAL   (United Kingdom)

Author keywords

Membrane fusion; Nuclear pore complex; Nucleoporin; Nucleus; Xenopus egg extracts

Indexed keywords

GLYCOPROTEIN; GLYCOPROTEIN GP 210; LAMIN B; LAMIN B3; MEMBRANE PROTEIN; NUCLEOPORIN; PROTEIN P62; UNCLASSIFIED DRUG;

ARTICLE; CELL ADHESION; CELL COMMUNICATION; CELL GROWTH; CELL NUCLEUS MEMBRANE; CELL STRUCTURE; CHANNEL GATING; COMPLEX FORMATION; CONTROLLED STUDY; INTRACELLULAR TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN TRANSPORT; SCANNING ELECTRON MICROSCOPY; TRANSMISSION ELECTRON MICROSCOPY; XENOPUS;

AMINO ACID SEQUENCE; ANIMALS; CELL MEMBRANE; CELL NUCLEUS; CYTOPLASM; DNA, COMPLEMENTARY; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLUTATHIONE TRANSFERASE; IMMUNOBLOTTING; INTERMEDIATE FILAMENT PROTEINS; LAMIN TYPE B; MEMBRANE FUSION; MEMBRANE GLYCOPROTEINS; MICROSCOPY, ELECTRON; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; NUCLEAR PORE COMPLEX PROTEINS; NUCLEAR PROTEINS; PROTEIN STRUCTURE, TERTIARY; RADIOMETRY; RECOMBINANT FUSION PROTEINS; THROMBIN; XENOPUS; XENOPUS PROTEINS;

EID: 0037043334     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200108145     Document Type: Article

References (61)

1. Yeast nuclear envelope proteins cross react with an antibody against mammalian pore complex proteins
2. The molecular mechanism of transport of macromolecules through nuclear pore complexes
3. The molecular machinery for secretion is conserved from yeast to neurons
4. Sequential recruitment of NPC proteins to the nuclear periphery at the end of mitosis
5. GTP hydrolysis is required for vesicle fusion during nuclear envelope assembly in vitro
6. Stepwise reassembly of the nuclear envelope at the end of mitosis
7. Membrane proteins of the nuclear pore complex: Gp210 is conserved in Drosophila, C. elegans and Arabidopsis
8. Spontaneous assembly of pore complex-containing membranes ("annulate lamellae") in Xenopus egg extract in the absence of chromatin
9. Nuclear pore complexes form immobile networks and have a very low turnover in live mammalian cells
10. Nuclear pore complex contains a family of glycoproteins that includes p62: Glycosylation through a previously unidentified cellular pathway
11. Lamina-associated polypeptide 2 isoforms and related proteins in cell cycle-dependent nuclear structure dynamics
12. The nuclear pore complex: Its free existence and a hypothesis as to its origin
13. Cell cycle-dependent phosphorylation of nucleoporins and nuclear pore membrane protein Gp210
14. Biochemical characterization of nuclear pore complex protein gp210 oligmers
15. A complex of nuclear pore proteins required for pore function
16. Analysis of nuclear lamin isoprenylation in Xenopus oocytes: Isoprenylation of lamin B3 precedes its uptake into the nucleus
17. The nucleoporin Nup98 associates with the intranuclear filamentous protein network of TPR
18. An antibody against a glycosylated integral membrane protein of the Xenopus laevis nuclear pore complex: A tool for the study of pore complex membranes
19. Roles of LAP2 proteins in nuclear assembly and DNA replication: Truncated LAP2β proteins alter lamina assembly, envelope formation, nuclear size, and DNA replication efficiency in Xenopus laevis extracts
20. Identification of a major polypeptide of the nuclear pore complex
21. Dimples, pores, star-rings, and thin rings on growing nuclear envelopes: Evidence for structural intermediates in nuclear pore complex assembly
22. Nuclear protein import is inhibited by an antibody to a lumenal epitope of a nuclear pore complex glycoprotein
23. A major glycoprotein of the nuclear pore complex is a membrane-spanning polypeptide with a large lumenal domain and a small cytoplasmic tail
24. Live fluorescence imaging reveals early recruitment ofemerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopes
25. Virus-cell and cell-cell fusion
26. GTP hydrolysis by Ran is required for nuclear envelope assembly
27. Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly
28. Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins
29. The control of DNA replication in a cell-free extract that recapitulates a basic cell cycle in vitro
30. An integral membrane protein from the nuclear pore complex is also present in the annulate lamellae: Implications for annulate lamella formation
31. Modification of the cytoplasmic domain of influenza virus hemagglutinin affects enlargement of the fusion pore
32. The human CAN protein, a putative oncogene product associated with myeloid leukemogenesis, is a nuclear pore complex protein that faces the cytoplasm
33. Mutations in the nuclear lamin proteins resulting in their aberrant assembly in the cytoplasm
34. Roles of cytoplasmic particles in nuclear envelope assembly and sperm pronuclear formation in cell-free preparations from amphibian eggs
35. Differential mitotic phosphorylation of proteins of the nuclear pore complex
36. Assembly of the nuclear pore: Biochemically distinct steps revealed with NEM, GTPγS, and BAPTA
37. A link between the synthesis of nucleoporins and the biogenesis of the nuclear envelope
38. The nuclear and the cytoplasmic pore complex: Structure, dynamics, distribution, and evolution
39. Formation and distribution of nuclear pore complexes in interphase
40. Nuclear pore complex assembly studied with a biochemical assay for annulate lamellae formation
41. The role of lamin LIII in nuclear assembly and DNA replication, in cell-free extracts ofXenopus eggs
42. Purification of the vertebrate nuclear pore complex by biochemical criteria
43. Egg extracts for nuclear import and nuclear assembly reactions
44. Characterization of the membrane binding and fusion events during nuclear envelope assembly using purified components
45. Toward the molecular details of the nuclear pore complex
46. The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex
47. Correlation between structure and mass distribution of the nuclear pore complex and of distinct pore complex components
48. Docking and fusion in neurosecretion
49. Separate nuclear import pathways converge on the nucleoporin Nup153 and can be dissected with dominant-negative inhibitors
50. 2+, inhibits lamin assembly, stabilizes nuclear architecture and may independently protect nuclei from apoptosis in vitro
51. Receptor binding and membrane fusion in virus entry: The influenza hemagglutinin
52. The large C-terminal region of the integral pore membrane protein, POM121, is facing the nuclear pore complex
53. Calcium mobilization is required for nuclear vesicle fusion in vitro: Implications for membrane traffic and IP3 receptor function
54. A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm
55. Nuclear pores and nuclear assembly
56. Gatekeepers of the nucleus
57. Nuclear envelope assembly in Xenopus extracts visualized by scanning EM reveals a transport dependent "envelope smoothing" event
58. The single transmembrane segment of gp210 is sufficient for sorting to the pore membrane domain of the nuclear envelope
59. Primary structure analysis of an integral membrane glycoprotein of the nuclear pore
60. Nucleocytoplasmic protein traffic and its significance to cell function
61. Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts