Metabolic engineering of the carotenoid biosynthetic pathway in the yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma)

Applied and Environmental Microbiology

Volumn 69, Issue 7, 2003, Pages 3728-3738

  Verdoes, Jan C ^a,c   Sandmann, Gerhard ^b   Visser, Hans ^a   Diaz, Maria ^a   Van Mossel, Minca ^a   Van Ooyen, Albert J J ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b GOETHE UNIVERSITY   (Germany)

^c GenoClipp Biotechnology bv   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; ENZYMES; GENES; METABOLISM; YEAST;

MUTANTS;

MICROBIOLOGY;

3 HYDROXY 3',4' DIDEHYDRO BETA CAROTEN 4 ONE; 3,4 DIDEHYDROLYCOPENE; ASTAXANTHIN; BETA CAROTENE; CAROTENOID; COMPLEMENTARY DNA; DIGOXIGENIN; ECHINONE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; LYCOPENE; LYCOPENE CYCLASE; PHOENICOXANTHIN; PHYTOENE; PHYTOENE DESATURASE; PHYTOENE SYNTHASE; PLASMID DNA; RECOMBINANT DNA; SYNTHETASE; TORULENE; UNCLASSIFIED DRUG;

BIOENGINEERING;

ARTICLE; BIOSYNTHESIS; CRTYB GENE; ESCHERICHIA COLI; FUNGAL GENE; GENE INACTIVATION; GENE OVEREXPRESSION; GENETIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHAFFIA RHODOZYMA; PLASMID; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; XANTHOPHYLLOMYCES DENDRORHOUS; YEAST;

ALKYL AND ARYL TRANSFERASES; BASIDIOMYCOTA; BETA CAROTENE; CAROTENOIDS; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENETIC ENGINEERING; INTRAMOLECULAR LYASES; MUTATION; OXIDOREDUCTASES; RECOMBINANT PROTEINS; XANTHOPHYLLS;

ESCHERICHIA COLI; PHAFFIA RHODOZYMA; XANTHOPHYLLOMYCES DENDRORHOUS;

EID: 0037479864     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.69.7.3728-3738.2003     Document Type: Article

References (36)

1. Albrecht, M., N. Misawa, and G. Sandmann. 1999. Metabolic engineering of the terpenoid biosynthetic pathway of Escherichia coli for the production of the carotenoids β-carotene and zeaxanthin. Biotechnol. Lett. 21:791-795.
2. An, G.-H., J. Bielich, R. Auerbach, and E. A. Johnson. 1991. Isolation and characterization of carotenoid hyperproducing mutants of yeast by flow cytometry and cell sorting. Bio/Technology 9:70-73.
3. An, G.-H., M.-H. Cho, and E. A. Johnson. 1999. Monocyclic carotenoid biosynthetic pathway in the yeast Phaffia rhodozyma (Xanthophyllomyces dendrorhous). J. Biosci. Bioeng. 88:189-193.
4. An, G.-H., C.-H. Kim, E.-S. Choi, and S.-K. Rhee. 1996. Medium optimization for cultivation of carotenoid hyperproducing Phaffia rhodozyma mutant HT-5FO1C. J. Ferment. Bioeng. 82:515-518.
5. Andrewes, A. G., H. J. Phaff, and M. P. Starr. 1976. Carotenoids of Phaffia rhodozyma, a red-pigmented fermenting-yeast. Phytochemistry 15:1003-1007.
6. Armstrong, G. A. 1997. Genetics of eubacterial carotenoid biosynthesis: a colorful tale. Annu. Rev. Microbiol. 51:629-659.
7. Breitenbach, J., N. Misawa, S. Kajiwara, and G. Sandmann. 1996. Expression in Escherichia coli and properties of the carotene ketolase from Haematococcus pluvialis. FEMS Microbiol. Lett. 140:241-246.
8. Chew, B. P., J. S. Park, M. W. Wong, and T. S. Wong. 1999. A comparison of the anticancer activities of dietary beta-carotene, canthaxanthin and astaxanthin in mice in vivo. Anticancer Res. 19:1849-1853.
9. Chumpolkulwong, N., T. Kakazono, S. Nagai, and N. Nishio. 1997. Increased astaxanthin production by Phaffia rhodozyma mutants isolated as resistant to diphenylamine. J. Ferment. Bioeng. 83:429-434.
10. De La Guardia, M. D., C. M. G. Aragón, F. J. Murillo, and E. Cerdá-Olmedo. 1971. A carotenogenic enzyme aggregate in Phycomyces: evidence from quantitative complementation. Proc. Natl. Acad. Sci. USA 68:2012-2015.
11. Fang, T. J., and Y.-S. Cheng. 1993. Improvement of astaxanthin production by Phaffia rhodozyma through mutation and optimization of culture conditions. J. Ferment. Bioeng. 75:466-469.
12. Farmer, W. R., and J. C. Liao. 2000. Improving lycopene production in Escherichia coli by engineering metabolic control. Nat. Biotechnol. 18:533-537.
13. Girard, P., B. Falconnier, J. Bricout, and B. Vladescu. 1994. β-Carotene producing mutants of Phaffia rhodozyma. Appl. Microbiol. Biotechnol. 41:183-191.
14. Hoshino, T., R. Fujii, and T. Nakahara. 1994. Overproduction of carotenoids in Thermus thermophilus. J. Ferment. Bioeng. 77:423-424.
15. Johnson, E. A., and W. A. Schroeder. 1995. Microbial carotenoids, p. 119-178. In A. Fiechter (ed.), Advances in biochemical engineering biotechnology, vol. 53. Springer-Verlag, Berlin, Germany.
16. Johnson, E. A., and W. A. Schroeder. 1995. Astaxanthin from the yeast Phaffia rhodozyma. Stud. Mycol. 38:81-89.
17. Kajiwara, S., P. D. Fraser, K. Kondo, and N. Misawa. 1997. Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli. Biochem. J. 324:421-426.
18. Mann, V., M. Harker, I. Pecker, and J. Hirschberg. 2000. Metabolic engineering of astaxanthin production in tobacco flowers. Nat. Biotechnol. 18:888-892.
19. Mayne, S. T. 1996. Beta-carotene, carotenoids and disease prevention in humans. FASEB J. 10:690-701.
20. Misawa, N., and H. Shimada. 1998. Metabolic engineering for the production of carotenoids in non-carotenogenic bacteria and yeasts. J. Biotechnol. 59:169-181.
21. Phaff, H. J., M. W. Miller, M. Yoneyama, and M. Soneda. 1972. A comparative study of the yeast florae associated with trees on the Japanese islands and on the west coast of North America, p. 759-774. In G. Terui (ed.), Proceedings of the 4th IFS: fermentation technology today. Society of Fermentation Technology, Kyoto, Japan.
22. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
23. Sandmann, G. 1994. Carotenoid biosynthesis in microorganisms and plants. Eur. J. Biochem. 223:7-24.
24. Sandmann, G., M. Albrecht, G. Schnurr, O. Knörzer, and P. Böger. 1999. The biotechnological potential and design of novel carotenoids by gene combination in Escherichia coli. TIBTECH 17:233-237.
25. Schmidt-Dannert, C., D. Umeno, and F. H. Arnold. 2000. Molecular breeding of carotenoid biosynthetic pathways. Nat. Biotechnol. 18:750-753.
26. Schmidt-Dannert, C. 2000. Engineering novel carotenoid in microorganisms. Curr. Opin. Biotechnol. 11:255-261.
27. Sedmak, J. J., D. K. Weerasinghe, and S. O. Jolly. 1990. Extraction and quantification of astaxanthin from Phaffia rhodozyma. Biotechnol. Technol. 4:107-112.
28. Verdoes, J. C., J. Wery, T. Boekhout, and A. J. J. van Ooyen. 1997. Molecular characterization of the glyceraldehyde-3-phosphate dehydrogenase gene of Phaffia rhodozyma. Yeast 13:1231-1242.
29. Verdoes, J. C., and A. J. J. van Ooyen. 1999. Isolation of isopentenyl diphosphate isomerase encoding gene of Phaffia rhodozyma; improved carotenoid production in Escherichia coli. J. Bot. Gall. 146:43-53.
30. Verdoes, J. C., N. Misawa, and A. J. J. van Ooyen. 1999. Cloning and characterization of the astaxanthin biosynthetic gene encoding phytoene desaturase of Xanthophyllomyces dendrorhous. Biotechnol. Bioeng. 63:750-755.
31. Verdoes, J. C., P. Krubasik, G. Sandmann, and A. J. J. van Ooyen. 1999. Isolation and functional characterisation of a novel type of carotenoid biosynthetic gene from Xanthophyllomyces dendrorhous. Mol. Gen. Genet. 262:453-461.
32. Wang, C.-W., M.-K. Oh, and J. C. Liao. 1999. Engineered isoprenoid pathway enhances astaxanthin production in Escherichia coli. Biotechnol. Bioeng. 62:235-241.
33. Wang, X., R. Willén, and T. Wadström. 2000. Astaxanthin-rich alga meal and vitamin C inhibit Helicobacter pylori infection in BALB/cA mice. Antimicrob. Agents Chemother. 44;2452-2457.
34. Wery, J., M. J. M. Dalderup, J. ter Linde, and A. J. J. van Ooyen. 1996. Structural and phylogenetic analysis of the actin gene from the yeast Phaffia rhodozyma. Yeast 12:641-651.
35. Wery, J., D. Gutker, A. C. H. M. Renniers, J. C. Verdoes, and A. J. J. van Ooyen. 1997. High-copy-number integration into the ribosomal DNA of the yeast Phaffia rhodozyma. Gene 184:89-97.
36. Wery, J., J. C. Verdoes, and A. J. J. van Ooyen. 1998. Efficient transformation of the astaxanthin-producing yeast Phaffia rhodozyma. Biotechnol. Technol. 12:339-405.