Cloning and characterization of the astaxanthin biosynthetic gene encoding phytoene desaturase of Xanthophyllomyces dendrorhous

Biotechnology and Bioengineering

Volumn 63, Issue 6, 1999, Pages 750-755

  Verdoes, Jan C ^a   Misawa, Norihiko ^b   Van Ooyen, Albert J J ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b KIRIN BREWERY CO LTD   (Japan)

Author keywords

Carotenoid; Complementation; Heterologous expression; Yeast

Indexed keywords

AMINO ACIDS; BACTERIA; BIOSYNTHESIS; CHARACTERIZATION; DNA SEQUENCES; ENZYMES; YEAST;

ASTAXANTHIN BIOSYNTHETIC GENE ENCODING; CAROTENOID; HETEROLOGOUS EXPRESSION; PHYTOENE DESATURASE; XANTHOPHYLLOMYCES DENDRORHOUS;

CLONING;

ASTAXANTHIN; CAROTENOID; ENZYME; FARNESYL TRANS TRANSFERASE; LYCOPENE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIUM TRANSFORMATION; BASIDIOMYCETES; ESCHERICHIA COLI; GENE EXPRESSION; GENE ISOLATION; GENETIC COMPLEMENTATION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; BASIDIOMYCOTA; BETA CAROTENE; CAROTENOIDS; CLONING, MOLECULAR; ESCHERICHIA COLI; FUNGAL PROTEINS; GENETIC COMPLEMENTATION TEST; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; XANTHOPHYLLS;

BACTERIA (MICROORGANISMS); BASIDIOMYCOTA; ERWINIA; ESCHERICHIA COLI; EUKARYOTA; PANTOEA ANANATIS; XANTHOPHYLLOMYCES DENDRORHOUS;

EID: 0033587374     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990620)63:6<750::AID-BIT13>3.0.CO;2-7     Document Type: Article

References (32)

1. Andrewes AG, Phaff HJ, Starr MP. 1976. Carotenoids of Phaffia rhodozyma, a red-pigmented fermenting yeast. Phytochemistry 15: 1003-1007.
2. Armstrong GA, Alberti M, Leach F, Hearst JE. 1989. Nucleotide sequence, organization and nature of the protein products of the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus. Mol Gen Genet 216:254-268.
3. Armstrong GA, Hearst JE. 1996. Genetics and molecular biology of carotenoid pigment biosynthesis. FASEB J 10:228-237.
4. Bramley PM. 1985. The in vitro biosynthesis of carotenoids. Adv Lipid Res 2:243-279.
5. Breter H-J, Knoop M-T, Kirchen H. 1987. The mapping of chromosomes in Saccharomyces cerevisiae. I. A cosmid vector designed to establish, by cloning into cdc-mutants, numerous start loci for chromosome walking in yeast genome. Gene 53:181-190.
6. Britton G. 1995. Structure and properties of carotenoids in relation to function. FASEB J 9:1551-1558.
7. Chamovitz D, Misawa N, Sandmann G, Hirschberg J. 1992. Molecular cloning and expression in Escherichia cou of a cyanobacterial gene coding for phytoene synthase, a carotenoid biosynthesis enzyme. FEBS Lett 296:305-310.
8. Demming-Adams B, Gilmore AM, Adams WW III. 1996. In vivo function of carotenoids in higher plants. FASEB J 10:403-412.
9. Ehrenshaft M, Daub ME. 1994. Isolation, sequence, and characterization of the Cercospora nicotianae phytoene dehydrogenase gene. Appl Environ Microbiol 60:2766-2771.
10. Girard P, Falconnier B, Bricout J, Vladescu B. 1994. β-Carotene producing mutants of Phaffia rhodozyma. Appl Microbiol Biotechnnol 41: 183-191.
11. Golubev WI. 1995. Perfect state of Rhodomyces dendrorhous (Phaffia rhodozyma). Yeast 11:101-110.
12. Johnson EA, Schroeder WA. 1995. Microbial carotenoids. In: Fiechter A, editor. Advances in Biochemical Engineering Biotechnology. Heidelberg: Springer-Verlag. Vol 53, p 119-178.
13. Johnson EA, Schroeder WA. 1996. Astaxanthin from the yeast Phaffia rhodozyma. Stud Mycol 38:81-91.
14. Linden H, Misawa N, Chamovitz D, Pecker I, Hirschberg J, Sandmann G. 1991. Functional complementation in Escherichia coli of different phytoene desaturase genes and analysis of accumulated carotenoids. Z Naturforsch 46C:1045-1051.
15. Linden H, Misawa N, Saito T, Sandmann G. 1994. A novel carotenoid biosynthesis gene encoding for ξ-carotene desaturase: Functional expression, sequence and phylogenetic origin. Plant Mol Biol 24: 369-379.
16. Matsumura H, Takeyama H, Kusakabe E, Grant Burgess J, Matsunaga T. 1997. Cloning, sequencing and expression the carotenoid biosynthesis genes, lycopene cyclase and phytoene desaturase, from the aerobic photosynthetic bacterium Erythrobacter longus sp. strain Och101 in Escherichia coli. Gene 189:169-174.
17. Mayne ST. 1996. β-Carotene, carotenoids, and disease prevention in humans. FASEB J 10:690-701.
18. Misawa N, Nakagawa M, Kobayashi K, Yamano S, Izawa Y, Nakamura K, Harashima K. 1990. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J Bacteriol 172:6704-6712.
19. Messing J. 1979. A multipurpose cloning system based on single-stranded DNA bacteriophage M13. Recomb DNA Tech Bull 2:43.
20. Olson JA, Krinsky NI. 1995. Introduction. The colorful, fascinating world of the carotenoids: Important physiologic modulators. FASEB J 9: 1547-1550.
21. Parker RS. 1996. Absorption, metabolism, and transport of carotenoids. FASEB J 10:543-551.
22. Pecker I, Chamovitz D, Linden H, Sandmann G, Hirschberg J. 1992. A single polypeptide catalyzing the conversion of phytoene to ξ-carotene is transcriptionally regulated during tomato fruit ripening. Proc Natl Acad Sci USA 89:4962-4966.
23. Rose RE. 1988. The nucleotide sequence of pACYC184. Nucleic Acids Res 16:355.
24. Ruiz-Hidalgo MJ, Benito G, Sandmann A, Eslava AP. 1997. The phytoene dehydrogenase gene of Phycomyces: Regulation of its expression by blue light and vitamin A. Mol Gen Genet 253:734-744.
25. Sandmann G. 1994. Carotenoid biosynthesis in microorganisms and plants. Eur J Biochem 233:7-24.
26. Sandmann G, Kowalczyk S. 1989. In vitro carotenogenesis and characterization of the phytoene desaturase reaction in Anacystis. Biochem Biophys Res Commun 163:916-921.
27. Schmidhauser TJ, Lauter FR, Russo VEA, Yanofsky C. 1990. Cloning, sequence, and photoregulation of al-1, a carotenoid biosynthetic gene of Neurospora crassa. Mol Cell Biol 10:5064-5070.
28. Verdoes JC, Wery J, Boekhout T, van Ooyen AJJ. 1997. Molecular characterization of the glyceraldehyde-3-phosphate dehydrogenase gene of Phaffia rhodozyma. Yeast 13:1231-1242.
29. Wery J, Dalderup MJM, ter Linde J, van Ooyen AJJ. 1996. Structural and phylogenetic analysis of the actin gene from the yeast Phaffia rhodozyma. Yeast 12:641-651.
30. Wery J, Gutker D, Renniers ACHM, Verdoes JC, van Ooyen AJJ. 1997. High-copy-number integration into the ribosomal DNA of the yeast Phaffia rhodozyma. Gene 184:89-97.
31. Wery J, Verdoes JC, van Ooyen AJJ. 1998. Efficient transformation of the astaxanthin-producing yeast Phaffia rhodozyma. Biotechnol Tech 12: 399-405.
32. Yanisch-Perron C, Vierra J, Messing J. 1985. Improved M13 phage cloning vectors and host strains; nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.